Yap1 (NM_001171147) Mouse Untagged Clone

CAT#: MC216820

Yap1 (untagged) - Mouse yes-associated protein 1 (Yap1), transcript variant 1, (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Yap1
• Synonyms: AI325207; Y; Yap; Yap65; Yk; Yki; yor; Yorkie
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC216820 representing NM_001171147
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCCGCGCAACAGCCGCCGCCCCAGCCGGCCCCGCAAGGCCCCGCGCCGCCGTCCGTGTCTCCGG
  CCGGGACCCCCGCGGCCCCGCCCGCACCCCCGGCCGGCCACCAGGTCGTGCACGTCCGCGGGGACTCGGA
  GACCGACTTGGAGGCGCTCTTCAATGCCGTCATGAACCCCAAGACGGCCAACGTGCCTCAGACCGTGCCC
  ATGCGGCTTCGCAAGCTGCCCGACTCCTTCTTCAAGCCGCCTGAGCCCAAGTCCCACTCGCGACAGGCCA
  GTACTGATGCAGGTACTGCGGGAGCTCTGACTCCACAGCATGTTCGAGCTCACTCCTCTCCAGCCTCCCT
  GCAGCTGGGTGCCGTTTCTCCTGGGACACTCACAGCCAGTGGCGTTGTCTCTGGCCCTGCCGCTGCCCCT
  GCAGCTCAGCATCTCCGGCAGTCCTCCTTTGAGATCCCTGATGATGTACCACTGCCAGCAGGCTGGGAGA
  TGGCCAAGACATCTTCTGGTCAAAGATACTTCTTAAATCACAACGATCAGACAACAACATGGCAGGACCC
  CCGGAAGGCCATGCTTTCGCAACTGAACGTTCCTGCGCCTGCCAGCCCAGCGGTGCCCCAGACGCTGATG
  AATTCTGCCTCAGGACCTCTTCCTGATGGATGGGAGCAAGCCATGACTCAGGATGGAGAAGTTTACTACA
  TAAACCATAAGAACAAGACCACATCCTGGCTGGACCCAAGGCTGGACCCTCGTTTTGCCATGAACCAGAG
  GATCACTCAGAGTGCTCCAGTGAAGCAGCCCCCACCCTTGGCTCCCCAGAGCCCACAGGGAGGCGTCCTG
  GGTGGAGGCAGTTCCAACCAGCAGCAGCAAATACAGCTGCAGCAGTTACAGATGGAGAAGGAGAGACTGC
  GGTTGAAACAACAGGAATTATTTCGGCAGGCAATACGGAATATCAATCCCAGCACAGCAAATGCTCCAAA
  ATGTCAGGAATTAGCTCTGCGCAGCCAGTTGCCTACACTGGAGCAGGATGGAGGGACTCCGAATGCAGTG
  TCTTCTCCTGGGATGTCTCAGGAATTGAGAACAATGACAACCAATAGTTCCGATCCCTTTCTTAACAGTG
  GCACCTATCACTCTCGAGATGAGAGCACAGACAGCGGCCTCAGCATGAGCAGCTACAGCATCCCTCGGAC
  CCCAGACGACTTCCTCAACAGTGTGGATGAAATGGATACAGGAGACACCATCAGCCAAAGCACCCTGCCG
  TCACAGCAGAGCCGCTTCCCCGACTACCTGGAAGCCCTCCCTGGGACAAATGTGGACCTTGGCACACTGG
  AAGGAGATGCAATGAACATAGAAGGGGAGGAGCTGATGCCCAGTCTGCAGGAAGCGCTGAGTTCCGAAAT
  CTTGGACGTGGAGTCTGTGTTGGCTGCCACCAAGCTAGATAAAGAAAGCTTTCTCACGTGGTTATAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001171147
• Insert Size: 1467 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171147.1, NP_001164618.1
• RefSeq Size: 4200 bp
• RefSeq ORF: 1467 bp
• Locus ID: 22601
• UniProt ID: P46938
• Cytogenetics: 9 A1
• Gene Summary: This gene encodes a protein which binds to the SH3 domain of the Yes proto-oncogene product, a tyrosine kinase. This protein contains a WW domain, consisting of four conserved aromatic amino acids including two tryptophan residues. This conserved WW domain is found in various structural, regulatory and signaling molecules in various species, and may play a role in protein-protein interaction. Following cellular damage, phosphorylation of this encoded protein may suppress apoptosis. This protein may be involved in malignant transformation in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.