Dbnl (NM_013810) Mouse Untagged Clone

SKU MC215709

Dbnl (untagged) - Mouse drebrin-like (Dbnl), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Dbnl
• Synonyms: Abp1; mAbp1; SH3P7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC215709 representing NM_013810; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGTGAACCTGAGCCGGAACGGGCCGGCGCTGCAGGAGGCCTACGTGCGCGTAGTCACCGAGAAAT; CCCCGACCGACTGGGCTCTTTTTACCTATGAAGGCAACAGCAATGACATCCGTGTGGCTGGCACAGGAGA; GGGAGGCCTGGAGGAGCTGGTGGAAGAGCTCAACAGCGGGAAGGTGATGTACGCCTTCTGCAGGGTGAAG; GACCCCAACTCCGGCCTGCCCAAGTTTGTCCTCATCAACTGGACAGGAGAGGGTGTGAATGATGTGCGGA; AAGGAGCATGTGCCAACCACGTCAGCACCATGGCCAACTTCCTGAAGGGTGCCCACGTGACCATCAATGC; CCGGGCCGAGGAGGATGTGGAGCCTGAGTGCATCATGGAGAAGGTTGCCAAGGCCTCTGGGGCCAACTAC; AGCTTCCATAAGGAAAGCACCTCCTTCCAGGATGTAGGGCCGCAGGCCCCAGTGGGCTCTGTGTACCAGA; AGACCAATGCCATATCTGAGATCAAGAGAGTCGGCAAGGATAACTTCTGGGCCAAAGCTGAGAAGGAAGA; AGAGAACCGCCGCCTGGAGGAGAAGCGGCGTGCCGAAGAGGAGCGGCAGCGGTTGGAGGAGGAGCGACGA; GAGCGGGAGCTGCAGGAGGCTGCCCGACGTGAGCAGCGCTACCAGGAACAGCACAGATCAGCTGGAGCCC; CGAGCAGGACAGGTGAGCCAGAGCAGGAAGCCGTTTCAAGGACCAGACAGGAGTGGGAGTCTGCTGGGCA; GCAGGCCCCACACCCACGAGAGATTTTCAAGCAGAAGGAAAGGGCAATGTCCACCACCTCTGTCACCAGC; TCGCAGCCGGGCAAGCTGAGGAGCCCCTTCCTGCAGAAGCAACTCACTCAACCAGAAACCTCCTACGGCC; GAGAGCCCACAGCTCCTGTCTCCCGGCCTGCAGCAGGTGTCTGTGAGGAGCCAGCGCCTAGCACTCTGTC; TTCTGCCCAGACAGAAGAAGAACCTACATATGAAGTACCCCCAGAGCAGGACACCCTCTATGAGGAACCA; CCACTGGTACAGCAGCAAGGGGCTGGCTCCGAACACATTGACAACTACATGCAGAGCCAGGGCTTCAGTG; GACAAGGGCTGTGCGCCCGGGCCTTGTATGACTACCAGGCAGCTGATGACACCGAGATCTCCTTTGACCC; TGAGAACCTAATCACAGGCATCGAGGTGATTGACGAAGGCTGGTGGCGAGGCTATGGGCCTGACGGCCAC; TTTGGCATGTTTCCTGCCAACTACGTGGAGCTCATAGAGTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_013810
• Insert Size: 1302 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013810.3, NP_038838.1
• RefSeq Size: 2360 bp
• RefSeq ORF: 1302 bp
• Locus ID: 13169
• UniProt ID: Q62418
• Cytogenetics: 11 3.87 cM
• Summary: Adapter protein that binds F-actin and DNM1, and thereby plays a role in receptor-mediated endocytosis. Plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. Does not bind G-actin and promote actin polymerization by itself. Required for the formation of organized podosome rosettes. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses a different splice site in its 3' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1.