Ccnh (NM_023243) Mouse Untagged Clone

SKU MC210415

Ccnh (untagged) - Mouse cyclin H (Ccnh), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Ccnh
• Synonyms: 6330408H09Rik; AI661354; AV102684; AW538719
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC210415 representing NM_023243; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTATCACAGCAGCAGCCAGAAACGGCACTGGACCTTCGCTAGCGAGGAGCAGCTGGCGCGCCTGCGGG; CGGACGCCAACCGCAAATTCAAGTGCAAAGCTGTGGCTAACGGGAAGGTTCTTCCAAATGATCCAGTGTT; TCTGGAGCCTCATGAAGAATTGACACTTTGCAAGTACTATGAAAAAAGATTATTGGAATTCTGTTCAGTG; TTTAAACCAGCTATGCCACGGTCTGTTGTGGGTACAGCTTGTATGTATTTCAAGCGTTTTTATCTTAATA; ACTCAGTAATGGAATATCACCCTCGGATAATAATGCTTACTTGTGCATTTTTGGCCTGCAAAGTAGATGA; ATTCAATGTGTCTAGTCCCCAGTTTGTTGGAAATCTTCGAGAGAGTCCTCTTGGACAGGAGAGGGCACTG; GAACAGATTTTGGAATATGAACTACTACTTATACAACAACTTAATTTTCACCTTATTGTCCACAATCCAT; ACAGACCATTTGAAGGCTTCCTCATCGATATAAAGACTCGGTACCCCATGTTGGAGAATCCGGAGATTCT; GAGGAAAACAGCTGATGATTTTCTTAGTAGAATTGCATTGACAGATGCTTATCTTTTATACACACCTTCA; CAGATTGCCCTGACCGCCATTTTATCAAGTGCCTCTAGAGCTGGAATTACTATGGAAAGCTACTTATCAG; AGAGTCTAATGCTAAAAGAAAACAGAACTTGCCTGTCACAGTTACTGGATATAATGAAAAGCATGAGAAA; TCTAGTAAAAAAGTACGAGCCGCCCAGGTCTGACGAGGTTGCTGTCCTGAAACAGAAGCTGGAGCGGTGT; CATTCTTCTGACCTTGCACTTAATGCGGTTACGAAGAAGAGGAAAGGCTATGAAGATGATGACTATGTGT; CAAAGAAACCCAAACAGGAAGAGGAGGAATGGACTGATGACGACCTGGTAGATTCTCTCTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_023243
• Insert Size: 972 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_023243.5, NP_075732.1
• RefSeq Size: 1976 bp
• RefSeq ORF: 972 bp
• Locus ID: 66671
• UniProt ID: Q61458
• Cytogenetics: 13 C3
• Summary: Regulates CDK7, the catalytic subunit of the CDK-activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).