Skp2 (NM_145468) Mouse Untagged Clone

SKU MC209794

Skp2 (untagged) - Mouse S-phase kinase-associated protein 2 (p45) (Skp2), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Skp2
• Synonyms: FBXL1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC209794 representing NM_145468; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCATAGGAAGCACCTTCAGGAGATTCCGGACCAGAGTGGCAACGTCACCACCAGCTTCACGTGGGGAT; GGGATTCCAGCAAGACTTCTGAACTGCTATCAGGCATGGGTGTCTCGGCCTTGGAGAAGGAGGAGGTGGA; CAGTGAGAACATCCCACATGGACTGCTCTCAAACCTCGGCCACCCCCAGAGCCCTCCAAGGAAACGAGTC; AAGGGCAAAGGGAGTGACAAAGACTTTGTGATCATCCGTCGGCCGAAGCTTAGTCGGGAGAACTTTCCAG; GTGTCTCCTGGGACTCCCTTCCAGATGAGCTGCTCCTTGGGATCTTTTCCTGTCTGTGCCTCCCTGAGCT; GCTGAGAGTCTCGGGCGTTTGCAAGAGGTGGTACCGCCTCTCGCTCGATGAGTCTCTCTGGCAGTCCCTC; GACCTCGCGGGTAAAAATCTGCACCCAGACGTGACTGTGCGCTTGCTCTCCCGCGGGGTGGTCGCCTTCC; GCTGCCCTCGGTCCTTTATGGAGCAGCCGCTGGGTGAAAGCTTCAGCTCTTTCCGGGTACAGCACATGGA; CCTGTCGAACTCAGTGATAAATGTGTCGAACCTCCATAAGATTCTGTCCGAGTGCTCCAAGCTGCAGAAT; CTAAGCCTGGAAGGCCTGCAGCTCTCAGACCCCATTGTCAAGACTCTTGCACAGAATGAAAACTTGGTGC; GACTAAACCTTTGTGGGTGCTCTGGGTTTTCTGAATCTGCCGTGGCGACTCTGCTAAGCAGCTGCTCCAG; ACTGGATGAGCTAAATCTCTCCTGGTGCTTTGACTTCACTGAAAAGCACGTGCAAGCGGCTGTGGCACAT; TTACCAAACACCATCACCCAGCTGAACCTCAGCGGCTACCGAAAGAACCTCCAGAAAACAGATCTTTGTA; CCATAATTAAACGATGCCCCAACCTCATCCGCCTCGACTTAAGTGACAGTATCATGCTAAAGAATGACTG; CTTTCCAGAATTTTTTCAACTCAACTACCTCCAACACCTCTCGCTCAGCCGGTGCTATGATATAATACCT; GATACTCTACTGTAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_145468
• Insert Size: 1065 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145468.1, NP_663443.1
• RefSeq Size: 6196 bp
• RefSeq ORF: 1065 bp
• Locus ID: 27401
• Cytogenetics: 15 A1
• Summary: Substrate recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. The SCF complex provides substrate specificity and interacts with both, the E2 ubiquitin-conjugating enzyme and the substrate. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration (By similarity).[UniProtKB/Swiss-Prot Function]