Csf2 (NM_009969) Mouse Untagged Clone
SKU
MC208342
Csf2 (untagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2), (10ug)
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
|---|---|
| Target Symbol | Csf2 |
| Synonyms | CSF; Csfgm; Gm-CSf; GMCSF; MGI-IGM |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites | SgfI-MluI | |
| ACCN | NM_009969 |
| Insert Size | 426 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | BC116880, AAI16881 |
| RefSeq Size | 618 bp |
| RefSeq ORF | 426 bp |
| Locus ID | 12981 |
| UniProt ID | P01587 |
| Cytogenetics | 11 32.13 cM |
| Summary | Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_009969" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG222594 | Csf2 (tGFP-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2), (10ug) | 10 ug |
$489.00
|
|
| MR222594 | Csf2 (Myc-DDK-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2) | 10 ug |
$289.00
|
|
| MR222594L1 | Lenti ORF clone of Csf2 (Myc-DDK-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2) | 10 ug |
$525.00
|
|
| MR222594L2 | Lenti ORF clone of Csf2 (mGFP-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2) | 10 ug |
$525.00
|
|
| MR222594L3 | Lenti ORF clone of Csf2 (Myc-DDK-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2) | 10 ug |
$525.00
|
|
| MR222594L4 | Lenti ORF clone of Csf2 (mGFP-tagged) - Mouse colony stimulating factor 2 (granulocyte-macrophage) (Csf2) | 10 ug |
$525.00
|
|
Citations
GM-CSF is key in the efficacy of vaccine-induced reduction of Helicobacter pylori infection
Vaillant, Oster, McMillan et al
Helicobacter (2022) 27 (2), e12875 DOI: 10.1111/hel.12875
Vaillant, Oster, McMillan et al
Helicobacter (2022) 27 (2), e12875 DOI: 10.1111/hel.12875
Cited by 5
AI Summary: OriGene products contributed to this study by providing the IL-22 expression plasmid (MR225471), GM-CSF expression plasmid (MC208342), and control plasmid (PS100001), which were used for hydrodynamic gene delivery in mice to investigate the roles of these cytokines in vaccine-induced reduction of Helicobacter pylori infection.
Snippet: 20 2.6 Hydrodynamic gene delivery injection Anesthetized mice received one injection of either 20 or 50 μg of IL‐22, GM‐CSF, and control expression plasmid (MR225471, MC208342, and PS100001 OriGene Technologies, respectively). … Bichsel AG) as described by;(MR225471, MC208342, and PS100001 OriGene Technologies, respectively)
Broad-Spectrum Therapeutic Suppression of Metastatic Melanoma through Nuclear Hormone Receptor Activation
Pencheva, Buss, Posada et al
Cell (2014) 156 (5), 986-1001 DOI: 10.1016/j.cell.2014.01.038
Pencheva, Buss, Posada et al
Cell (2014) 156 (5), 986-1001 DOI: 10.1016/j.cell.2014.01.038
Cited by 174
AI Summary: OriGene’s pCMV6-GM-CSF vector (MC208342) was used to engineer B16F10 melanoma cells to overexpress GM-CSF, enabling the study of its effects on melanoma progression and metastasis in this research.
Snippet: B16F10 cells were engineered to overexpress GMCSF by transfection of 2 mg of pCMV6-GM-CSF vector (MC208342, OriGene, Rockville, MD) into B16F10 cells 48 hr prior to injection
Immune-dependent and independent antitumor activity of GM-CSF aberrantly expressed by mouse and human colorectal tumors
Urdinguio, Fernandez, Moncada-Pazos et al
Cancer Res. (2013) 73 (1), 395-405 DOI: 10.1158/0008-5472.CAN-12-0806
Urdinguio, Fernandez, Moncada-Pazos et al
Cancer Res. (2013) 73 (1), 395-405 DOI: 10.1158/0008-5472.CAN-12-0806
Cited by 2
AI Summary: OriGene’s pGFP-V-RS vector carrying Mus musculus Csf2 and the HuSH 29-mer Non-Effective Scrambled pGFP-V-RS vector were used to engineer colon tumor cells for functional studies of GM-CSF expression and its effects in this research.
Snippet: musculus Csf2 in pGFP-V-RS vector or a HuSH 29-mer Non-Effective Scrambled pGFP-V-RS vector (OriGene) following the ... Millipore) overnight, 4°C. After rinsing with Dulbecco's phosphate-buffered saline, slides were incubated with Alexa 594 (A11072, Life Technologies)
Showing 1-3 of 3 papers.
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