Pdgfa (NM_008808) Mouse Untagged Clone

SKU MC208036

Pdgfa (untagged) - Mouse platelet derived growth factor, alpha (Pdgfa), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Pdgfa
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC208036 representing NM_008808; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGGACCTGGGCTTGCCTGCTGCTCCTCGGCTGCGGATACCTCGCCCATGCCCTGGCCGAGGAAGCCG; AGATACCCCGGGAGTTGATCGAGCGGCTGGCTCGAAGTCAGATCCACAGCATCCGGGACCTCCAGCGACT; CTTGGAGATAGACTCCGTAGGGGCTGAGGATGCCTTGGAGACAAGCCTGAGAGCCCATGGGTCCCATGCC; ATTAACCATGTGCCCGAGAAGCGGCCTGTGCCCATTCGCAGGAAGAGAAGTATTGAGGAAGCCATTCCCG; CAGTTTGCAAGACCAGGACGGTCATTTACGAGATACCTCGGAGCCAGGTGGACCCCACATCGGCCAACTT; CCTGATCTGGCCCCCATGTGTGGAGGTGAAGCGCTGCACTGGCTGTTGTAACACCAGCAGCGTCAAGTGC; CAGCCTTCACGGGTCCACCACCGCAGTGTCAAGGTGGCCAAAGTGGAGTATGTCAGGAAGAAGCCAAAAT; TGAAAGAGGTCCAGGTGAGGTTAGAGGAACACCTGGAGTGTGCATGTGCGACCTCCAACCTGAACCCAGA; CCATCGGGAGGAGGAGACAGATGTGAGGTGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI
• ACCN: NM_008808
• Insert Size: 591 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_008808.3, NP_032834.1
• RefSeq Size: 1019 bp
• RefSeq ORF: 591 bp
• Locus ID: 18590
• UniProt ID: P20033
• Cytogenetics: 5 77.65 cM
• Summary: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal lung alveolar septum formation during embryogenesis, normal development of the gastrointestinal tract, normal development of Leydig cells and spermatogenesis. Required for normal oligodendrocyte development and normal myelination in the spinal cord and cerebellum. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFB.[UniProtKB/Swiss-Prot Function]