Sppl3 (NM_029012) Mouse Untagged Clone

SKU MC207744

Sppl3 (untagged) - Mouse signal peptide peptidase 3 (Sppl3), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Sppl3
• Synonyms: 4833416I09Rik; Psl4; Usmg3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >MC207744 representing NM_029012; Red=Cloning site Blue=ORF Orange=Stop codon; ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAACAGACCTATTCGTGGGCCTATTCCCTGGTGGACTCCAGTCAAGTGTCGACATTTCTGATAT; CCATTCTTCTTATAGTCTATGGTAGTTTCAGGTCTCTTAATATGGACTTTGAAAACCAAGATAAGGAGAA; AGACAGCAACAGTTCTTCTGGCTCTTTCAATGGCAACAGCACCAATAACAGCATCCAGACCATTGATTCC; ACCCAAGCACTGTTCCTCCCGATTGGAGCGTCTGTCTCTCTCCTCGTCATGTTCTTCTTCTTTGATTCAG; TTCAAGTCGTTTTCACAATATGTACAGCAGTTCTTGCAACAATAGCTTTTGCTTTTCTTCTTCTCCCGAT; GTGCCAGTATTTAACAAGGCCCTGCTCACCTCAGAACAAGATTTCCTTCGGTTGCTGTGGGCGTTTCACT; GCTGCCGAGCTGCTGTCGTTCTCCCTGTCTGTCATGCTCGTCCTCATCTGGGTTCTCACTGGCCACTGGC; TTCTCATGGATGCTCTGGCCATGGGTCTCTGTGTTGCCATGATCGCCTTCGTCCGCCTGCCAAGCCTCAA; GGTTTCCTGCCTGCTTCTCTCAGGGCTTCTCATCTACGATGTCTTCTGGGTGTTCTTCTCAGCCTACATC; TTCAACAGTAATGTCATGGTGAAAGTGGCCACACAGCCAGCTGACAATCCCCTCGACGTTCTGTCCAGGA; AGCTCCACCTGGGACCCAATGTGGGGCGTGATGTTCCTCGCCTGTCTTTGCCTGGAAAATTGGTCTTCCC; AAGCTCCACTGGCAGTCACTTCTCTATGCTGGGCATCGGGGACATTGTGATGCCCGGCCTCCTGTTATGC; TTTGTTCTTCGCTATGACAACTACAAGAAACAAGCCAGTGGTGACTCGTGTGGGGCCCCTGGCCCAGCCA; ATATCTCTGGGCGCATGCAGAAGGTCTCCTACTTCCACTGCACCCTCATCGGGTACTTTGTAGGTCTGCT; CACTGCGACTGTGGCGTCTCGCATCCACCGAGCTGCCCAGCCAGCTCTCCTCTACTTGGTGCCATTTACC; CTATTGCCACTCCTCACCATGGCCTACCTAAAGGGTGACTTACGGAGGATGTGGTCTGAGCCCTTCCACT; CCAAGTCCAGCAGCTCCCGGTTCCTGGAAGTATGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_029012
• Insert Size: 1155 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_029012.2, NP_083288.2
• RefSeq Size: 2817 bp
• RefSeq ORF: 1155 bp
• Locus ID: 74585
• UniProt ID: Q9CUS9
• Cytogenetics: 5 F
• Summary: Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane protein substrates in or close to their luminal transmembrane domain boundaries. Acts like a sheddase by mediating the proteolytic release and secretion of active site-containing ectodomains of glycan-modifiying glycosidase and glycosyltransferase enzymes such as MGAT5, B4GAT1 and B4GALT1 (PubMed:25354954, PubMed:25827571). Plays a role in the regulation of cellular glycosylation processes (PubMed:25354954). Required to link T-cell antigen receptor (TCR) and calcineurin-NFAT signaling cascades in lymphocytes by promoting the association of STIM1 and ORAI1 during store-operated calcium entry (SOCE) in a protease-independent manner (PubMed:25384971).[UniProtKB/Swiss-Prot Function]