Pnck (NM_012040) Mouse Untagged Clone

SKU MC205474

Pnck (untagged) - Mouse pregnancy upregulated non-ubiquitously expressed CaM kinase (Pnck), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Pnck
• Synonyms: Bstk3; Camk1b; CaMKIbeta2; caMKlb1; CaMKlb2; Punc
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC055891; GCGGCAGCGGCGGTGGCAGCAGCTGCAGACATGCTGCTGCTCAAGAAACAGACGGAGGACATCAGCAGTG TCTATGAGATCCGGGAGAAGCTGGGCTCGGGTGCCTTCTCTGAGGTGATGCTGGCCCAGGAAAGGGGCTC TGCTCATCTTGTGGCCCTCAAGTGCATTCCCAAGAAAGCACTTCGGGGCAAGGAGGCCCTGGTGGAGAAT GAGATCGCGGTACTTCGCAGAATCAGCCATCCCAACATTGTGGCTCTGGAGGACGTCCATGAGAGTCCTT CTCATCTCTACTTGGCCATGGAGCTGGTAACAGGTGGTGAACTGTTTGACCGCATCATGGAGCGGGGCTC CTACACAGAGAAGGACGCCAGCCACCTTGTAGGGCAGGTCCTTGGCGCTGTCTCCTACCTTCATAGCCTG GGCATCGTGCACCGGGACCTCAAGCCTGAAAACCTCCTCTATGCCACACCTTTTGAGGACTCCAAGATCA TGGTCTCTGACTTTGGCCTGTCCAAAATACAAGCTGGCAACATGCTAGGCACAGCCTGTGGGACCCCAGG ATATGTGGCCCCAGAGCTCCTGGAGCAGAAACCCTACGGGAAGGCCGTAGATGTGTGGGCCCTGGGTGTC ATCTCCTACATCCTGCTGTGTGGGTACCCCCCCTTCTATGATGAGAGCGATCCTGAACTCTTCAGCCAGA TTCTGAGGGCCAGCTATGAGTTTGACTCCCCCTTTTGGGATGACATCTCAGAATCAGCCAAAGACTTCAT TCGCCACCTTCTGGAACGTGATCCCCAGAAGAGGTTCACCTGCCAGCAGGCCCTACAGCATCTTTGGATC TCTGGGGATGCAGCCTTCGATAGGGACATCCTGGGTTCTGTCAGTGAGCAGATCCAGAAGAATTTTGCCA GGACCCACTGGAAGCGTGCATTCAATGCCACATCATTCCTACGTCACATCCGTAAGCTGGGACAAAGCCC AGAGGGTGAGGAGGCCTCCAGGCAGTGTATGACCCGTCATAGCCACCCAGGCCTTGGGACTAGCCAGTCC CCCAAGTGGTGAAAACCAGGTAGATGCCAAGGAAGGCCAAGTGGACTGACTCCCGGTTTTTCTTTCCTCC AGCCCTTTTGGTCTCTTTCCTGGATCCTTGTCCTCCAGACTGGCCTCTGCTGGAAAGTCTGAGACTGGGT GTGATGCATGGCACTAGGGTACGGGGCTTCCCCAGTATGTCCCCCAGCCTCTATTCTTACCTATGGTGGA GGCTCCCTTTCCCATGTCGCTGCCACCCTCTATGGAAACTGAGGAGGTGTTCAAAAGTGGACTTGGGAGC CATCCTTCCTGCACCTTGCACGAACACATGCATTGTGTGGCTGTTCTGTGCTTTGCTGACTGTGGGTGGT CCTGCTTGTGTTGTGGCCCTTTAGTTCCTCCTTTTCCTAACCAATAAAGACAAACAGAACCATAAAAAAA AAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_012040
• Insert Size: 1032 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC055891, AAH55891
• RefSeq Size: 1489 bp
• RefSeq ORF: 1032 bp
• Locus ID: 93843
• UniProt ID: Q9QYK9
• Cytogenetics: X 37.38 cM
• Summary: Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses a different splice site in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.