Asic1 (NM_009597) Mouse Untagged Clone

SKU MC205227

Asic1 (untagged) - Mouse amiloride-sensitive cation channel 2, neuronal (Accn2), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Asic1
• Synonyms: Accn2; AI843610; ASIC; ASIC1a; B530003N02Rik; BNaC2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC067025; CCGAGCTGAGCCGAGCTGGCCGAGCGGGCCGCTCCCTGCAGGGCTCTACGCGGGTGCCGCGGCAGCCGGC GGCTCGGGCTCCGGGCCATGAGCCCCTCCGCCGCGCGGCGCTGAGCCCACGGACCGCCTCGCGCCCCAGG ACCTGCAGCGGCGGCCGGCTTTCCCCAGCCCCCTCAGGATCCCCTTGGCAAGCATGGAACTGAAGACCGA GGAGGAGGAGGTGGGTGGTGTCCAGCCGGTGAGCATCCAGGCTTTTGCCAGCAGCTCCACGCTGCACGGT CTTGCCCACATCTTCTCCTATGAGCGGCTGTCTCTGAAGCGGGCACTGTGGGCCCTGTGTTTCCTGGGTT CGCTGGCCGTCCTGCTGTGTGTGTGCACTGAGCGTGTGCAGTACTACTTCTGCTACCACCACGTCACCAA GCTCGACGAGGTGGCTGCCTCCCAGCTCACCTTCCCTGCCGTCACTCTCTGCAACCTCAATGAGTTTCGC TTTAGCCAAGTCTCCAAGAATGACCTGTACCATGCTGGGGAACTGCTGGCCCTGCTCAACAACAGGTATG AGATACCGGACACACAGATGGCTGATGAAAAGCAGCTGGAGATATTGCAGGACAAGGCCAACTTCCGTAG CTTCAAGCCCAAGCCCTTCAACATGCGTGAGTTCTACGACAGAGCAGGGCATGACATTCGAGACATGCTT CTCTCGTGCCACTTCCGAGGGGAGGCCTGCAGCGCTGAAGACTTCAAAGTGGTCTTCACGCGGTATGGGA AGTGCTACACATTCAACTCGGGCCAAGATGGGCGGCCACGGCTGAAGACCATGAAAGGTGGGACTGGCAA CGGCCTGGAGATCATGCTGGACATTCAGCAAGATGAATACTTGCCTGTGTGGGGAGAGACTGATGAGACA TCGTTCGAAGCAGGCATCAAAGTGCAGATCCACAGTCAGGACGAGCCTCCTTTCATCGACCAGCTGGGCT TTGGCGTGGCCCCAGGCTTCCAGACGTTTGTGTCTTGCCAGGAGCAGAGGCTCATCTACCTGCCCTCCCC CTGGGGCACCTGCAATGCTGTTACCATGGACTCGGATTTCTTCGACTCCTACAGCATCACGGCCTGCCGG ATTGATTGTGAAACCCGTTACCTGGTGGAAAACTGCAACTGCCGTATGGTGCACATGCCAGGGGATGCCC CATACTGTACTCCGGAGCAGTACAAGGAGTGTGCAGACCCTGCCCTGGACTTCCTAGTGGAGAAAGACCA GGAATACTGTGTGTGTGAGATGCCCTGCAACCTGACCCGCTACGGCAAGGAGCTGTCCATGGTCAAGATC CCCAGCAAAGCCTCAGCCAAGTACCTGGCCAAGAAGTTCAACAAATCTGAACAGTACATAGGGGAGAATA TTCTGGTGCTGGACATTTTCTTTGAAGTCCTCAACTATGAGACCATCGAGCAGAAGAAGGCCTATGAGAT CGCAGGGCTTTTGGGTGACATCGGGGGCCAGATGGGATTGTTCATCGGGGCCAGCATCCTCACAGTGCTG GAACTCTTTGACTATGCCTATGAGGTCATTAAGCACCGGCTGTGTAGACGTGGGAAGTGCCAGAAGGAGG CTAAGAGGAACAGCGCAGATAAGGGCGTGGCGCTCAGCCTGGATGACGTCAAAAGACACAATCCCTGCGA GAGCCTCCGAGGACATCCTGCCGGGATGACGTACGCTGCCAACATCCTACCTCACCATCCCGCTCGAGGC ACGTTTGAGGACTTTACCTGCTAAGCCCTCGCCAGCCGCTGTACCAAAGGCCTAGGTGGGGAGGGCTGGG GGAGCAAGGGGTACCTAACTGCCCCCAGCTACCCTGGGGACTTAAACTGCATCCCTGGGCAGTGGTTCCC TCTTGTCTGTGGTGAGAAAGGAGTCTTGACCAGAGTCCTCTCCCAGCCTCTATCTCATCTTTTTATTTTA ACAAAATTAAACTAATATAAAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATCAGCCGCG ACCTCAGGCTGCCCCTCTGCTCCACGCTGCCGCCCCCAGCTCCAAGCCTGAATTCTGTCTGTCTGGCTGT ATGTCATCTGAGTGTCCACCTACATTCTGCTGCCTCCAGTCACCAAAGCCCCCTCCTAGTGAGGGGTGGA GGGGATCTCTAGGGTCTGAAATTTGGCCCCAAACCAGAGAATGTACCTTAAGGGAGAGGGCTGGTGTGGG GGCTTCCCTAGCCTTAAGAGATGCTCTCAGCCGGGTGACTCCCCACGAGCCCAAGTCTCCAGGTAAGGAC TCCGTGCCCAGTTACTGCTCCCTCAAGCATACGGAATCTTTACGGGGCAGAGGATTTCCTGAAGAAGTAG AGATTGGGACTCCATGTGGCCCTGGTGCTGTAGGCCACATCCCAATATCTGTAAGTCACCTCCACCCCAA AGCTGCTGGAGAGAGATCCCAAGAGGCAACCCTCTGTGACATCCCCCAGAAGACCTGGCTGGTTAGCGCC CGGCTCAGGGAGAGGGGCACGGCCGCCTGACCTCACTGGCTTCTCTCTCAGAGGCCTTTGCAGAAGGCCA CATCCATAAATTTTCTTATGGAACAATCCCAAGTCCTCTTCCCCGACGTCATCCGCTTCTCTCGGCAACC TCATCTGCATTTTCTATTTCTATATGATACAGACTCTATATTACTATAACCTTGTATATACTCCCCTCCG GCCCTGTTTGTCTCCCCCTCCATTCCCTCTTGTCTCCAAGAACCGCCCTCCCGCCCCTAGTTCTCCCTTC TGTCTCTCCCCCATTCTCCTGTCTCTGAATGCCTTTGCCCGTATAAAGAGCTGGATTCTCCCCTGGTGTC TGTACTGTACTGTGTACACACATCCCTCTGAGAAGCACAAGGAGATGACACGCGCATTGTAACCTTCGCA CTGTCTCGGTGGCGACATAAAGGAAGCTGTGAATTACAAGCTCTGCCTCTTTCTGGCCTCGCCCTTTGCC CCCACGACCGGGGCACCCTCTGCCCTCCCCACAGCCTTAACATACCCTCTACCTTGCTCCACCTATCCCA GTGCCCTTTGCCTGGCTGACTGTGGCCTCCCTGAGGGAAGTGACTGCTGGAGAGGTAGCTCCTACCCAGG AATGGAGGACACTAAGCGGAGAGAGTTACAGACGGGAGGTGGGGAGGGAGGCTGGGGATTGGTGACTCCC TCTTGCAGCTGGGCGGTGGGTACCCACAGCCAGAAGCCGGAGCCCTGACTCAGTGGCCTTTCTTTCTTCG GGGTGCCCAGGGCCCAGTCCTCACCTTGATGCCCAGGCCCACCGCTTGCATTTTCTGGTGAGTCAGGGCG TGGTCACATGAGGAAAGAGATTGGCCTAGAGCAGAAGTTGAAAGACGTGCTGTTGTGTGTCCCTACCCTA AGGGGGCCACAAGGTGAGGTAGAGGGCCTCCTCAACCAGACACCCTTCCCAGGCCAGCTCTCCAATCTCA TTCCTGGCCTTCTCTGTCCCCACTGTCTCTTGGTCTCTAATCCCAAAACAGGACTTATGGGCAGATCACC TGTGTACGTTGGCCTGGAGCCAGAGAGCAAGGCTTTGGCATCTTTGAGACCTCTTGGTTCTCAAGAGTTG TTTAGAGATCAGAACTCCCCAGAAAGAAACAGGGCAAAGCTGAGAAATGTACACTGGCTGGGGGGGCAGC AGGAGGGGCTCTGGGTGACGCATGCCTCTGGTCTAATAAACTGGGTTTCAACCAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_009597
• Insert Size: 1581 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC067025, AAH67025
• RefSeq Size: 3800 bp
• RefSeq ORF: 1581 bp
• Locus ID: 11419
• UniProt ID: Q6NXK8
• Cytogenetics: 15 F1
• Summary: Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. Generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Can also transport potassium ions, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear.[UniProtKB/Swiss-Prot Function]