Tyw1 (NM_001015876) Mouse Untagged Clone

SKU MC205198

Tyw1 (untagged) - Mouse tRNA-yW synthesizing protein 1 homolog (S. cerevisiae) (Tyw1), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Tyw1
• Synonyms: AW125391; Rsafd1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC068126; TTCGCTGTCCCTCTCTCTTTGCGTTTGCCGGTTGACTTGCGTAGCTCGGGCCCCGCGGAGGATGGGTCCT CTGGATGTGTGGGACTTGTCGCCTTTGCTGTCACTGTGGATGAACAGGTTCTACATATATATGGGCTGTG CCCTGGGCCTTACCCTTTGCATCTGTGTCCAGATCATCAAGAAGCAGGTCACTCGTTCCCAGGAGAAACG TGTCCCAGGAGCTCCTGATAGCTCCCTCTCCCCTCAGAAGAAGCAGACACACGTTTCTGGAGTGAAGATT TTTTACGGATCTCAGACGGGCACTGCCAAGGGATTTGCAGTGGTTCTTGCCAAAGCTGTCACCTCTCTGG ACCTGCCTGTGGCCATTATTAATCTGAAGGAATATGATCCAGATGACAGTCTGATAGGAGAGATCACTAG TAAAACCGTCTGTGCCTTCCTGGTCGCTACGTACACGGACGGCTGCCCTACAGAGAGTGCAGAGTGGTTC TGCAAGTGGTTAGAGGAATCAGCTAATGATTTCCGCTTTGGCAAAACTTACCTGAAGGGACTTCGCTATG CAGTGTTTGGCCTGGGAGACTCTGCCTACCGGAGCCACTTCAACAAGGTCAGCACGAACGTTGACAAATG GCTCTGGATGCTCGGTGCCCAGCGGGTGCTGACCCGAGGGGAAGGCGACTGCAATGCAGTTCAGAGCAAA CATGGCAGCATCGAGGCCGACTTCACAGCCTGGAAGACCAAGTTCATCTCCCGGCTGCAGGCCCTGCAGA GAGGGGAGAAAAAGGCTTGTGGCGGGAACTGCAAGAGAGGGAAGTGTGAGTCGGCCCAGCATGGCCCAGG AGAGGCGCGGCCTCACCCGCAAGGCGAATTGCATCCTGGGGATGCTGAGGAGGAAGAGCCCTGTGAGAGC AGCAGCGAGGACGAGCTCGGGACCCAGGATTATCAGAGCCTGACTTCTGTGGTGGATGTTGAGGACCTGG GCAACATCATGAACCCCGTGAAGAGGGAAAAGAGAGAAAAGTCTCATCAGGACGGGAAAGCTGCCATGCA GAGGAACCCAGAGAAGACTGAGGACGGCGAAGGAAGAGCTATGATAACACCTGCTCTGCGGGAAGCCCTG ACTAAACAAGGGTACCAGCTGATTGGCAGCCACTCTGGTGTGAAGCTTTGCAGGTGGACAAAGTCGATGC TGCGGGGGAGAGGAGGTTGCTACAAACACACATTCTACGGCATTGAGAGCCACCGCTGCATGGAAGCCAC CCCAAGCTTGGCGTGTGCGAATAAATGTGTCTTCTGCTGGCGGCACCATACCAATCCTGTGGGCACTGAG TGGCGGTGGAAGATGGACCAGCCAGAATTGATCTTGAAGGAAGCCATTGAGAACCATCAGAACATGATTA AACAGTTCAAAGGCGTGCCAGGCCTCAAGGCGGAGCGCTTTGAAGAGGGGATGGAGGTCAAGCACTGTGC GCTGTCACTCGTGGGTGAGCCGATCATGTACCCAGAAATCAACAGGCTTTTGAAGCTGCTCCACCAGCAC GGCATCTCCAGCTTCCTTGTCACCAATGCGCAGTTCCCTGAGGAGATCAGGAAGCTCACACCAGTTACTC AGCTCTACGTGAGCGTGGACGCCAGCACCAGAGACGGCCTGAAGAAGATCGACCGCCCGCTCTTCAAGGA TTTCTGGCAGCGCTTCCTAGACAGTTTGAAAGCCTTATCTGCAAAGCAACAACGAACGGTCTACAGACTG ACGCTTGTGAAGTGTTGGAACGTGGATGAACTCCAGGCCTACGCTGAGCTGGTGTCCTTGGGGAACCCCG ACTTCATCGAGGTCAAGGGTGTGACCTACTGTGGTGAGAGTGCAGCCAGCAGCCTCACCATGGCCAATGT CCCTTGGCACGAGGAAGTGGTTCGGTTTGTCCGTGAGCTGGTCGATCTGCTTCCCGACTATGAAGTTGCG TGTGAACACGAGCACTCCAACTGCCTCCTGATCGGACACAAGAAGTTTAAGATCGATGGAGAATGGTGGA CATGGATCAATTACAGCCGCTTCCAAGAGCTCGTTCTGCAGTACGAAGAGAGTGGAGGCTCCAAGACGTT CAGCTCGAGGGATTACATGGCCAGAACCCCACAGTGGGCACTGTTTGGTGCCAGGGAAAGAGGCTTTGAT CCCAAGGACACGAGATACCAGAGGAAGAACAAAACGAAGGACATTTCCGGATGTTGAGCTTATCTGAGTT AAGGATCTCCAAAGACAAAAGCTGCGTGGCCCCGGCAGGTCCTTGGCTGCCATCACTGTCCACGGGGCAA CGGTTCACAGCCGTACTTCATGGTCTGACACCAGCAGGCAGCCATGCTGCCCACACAGCATCCACAGGTC GACGCTGCTTACCAGAACTCATGCCCTTTAAAGAGTCAGTTTCTGAGACAGTGAAGGTGACAGTCACCTG AATTTTTACAGTTTGGGTTTTATTTTAGTAATTTTCCTAAGTGCTATCCCTGAGTGTTACACAGGGAATA AACACTGCCGCCTCCGCCTCCTGAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_001015876
• Insert Size: 2166 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC068126, AAH68126
• RefSeq Size: 2564 bp
• RefSeq ORF: 2166 bp
• Locus ID: 100929
• UniProt ID: Q8BJM7
• Cytogenetics: 5 G1.3
• Summary: Probable component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine, an intermediate in wybutosine biosynthesis (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longer isoform (1).