Cybb (NM_007807) Mouse Untagged Clone

CAT#: MC204867

Cybb (untagged) - Mouse cytochrome b-245, beta polypeptide (Cybb), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Cybb
• Synonyms: C88302; Cgd; CGD91-phox; Cyd; gp91-1; gp91
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC071229
  GGCAGAACCAACACTTAACCTTTGCTAACATGGGGAACTGGGCTGTGAATGAAGGACTCTCCATCTTTGT CATTCTGGTGTGGTTGGGGCTGAATGTCTTCCTCTTTATCAACTACTACAAGGTTTATGATGATGGGCCT AAGTATAATTATACTCGAAAACTCCTTGGGTCAGCACTGGCTCTGGCCCGGGCACCTGCAGCCTGCCTGA ATTTCAACTGTATGCTGATCCTGCTGCCAGTGTGTCGAAATCTGCTCTCCTTTCTCAGGGGTTCCAGTGC GTGTTGCTCGACAAGGATTCGAAGACAACTGGACAGGAACCTCACTTTCCATAAGATGGTAGCTTGGATG ATAGCACTGCACACCGCCATCCACACAATTGCACATCTCTTCAACGTTGAGTGGTGTGTGAATGCCAGAG TCGGGATTTCTGACCGGTATTCAATAGCTCTCTCTGACATCGGTGACAATGAGAACGAAGAGTATCTCAA TTTTGCTAGAGAAAAAATCAAGAACCCTGAAGGGGGCCTGTATGTGGCCGTGACTCGGTTGGCTGGGATC ACAGGAATTGTCATCACACTGTGTCTCATATTGATTATCACATCCTCTACCAAAACCATTCGGAGGTCTT ATTTTGAAGTGTTCTGGTATACACACCATCTCTTTGTGATCTTCTTCATCGGCCTTGCCATCCATGGAGC TGAACGAATTGTACGTGGACAGACTGCGGAGAGTTTGGAAGAGCATAATTTAGATATCTGTGCAGACAAA ATCGAAGAATGGGGAAAAATAAAGGAGTGCCCAGTACCAAAGTTCGCTGGAAACCCTCCTATGACTTGGA AATGGATAGTGGGTCCTATGTTCCTGTACCTTTGTGAGAGGTTGGTTCGGTTTTGGCGATCTCAGCAAAA GGTGGTCATCACCAAGGTGGTCACCCACCCTTTCAAAACCATTGAGCTTCAGATGAAGAAGAAGGGATTC AAGATGGAGGTGGGACAGTACATTTTTGTCAAGTGCCCCAAGGTATCCAAGTTAGAATGGCACCCATTCA CACTGACCTCTGCTCCTGAGGAAGACTTTTTTAGCATCCATATCCGTATTGTGGGAGACTGGACGGAGGG GCTATTCAATGCTTGTGGCTGTGATAAGCAGGAGTTCCAAGATGCCTGGAAACTACCTAAGATAGCAGTT GATGGACCCTTTGGTACAGCCAGTGAAGATGTGTTCAGCTATGAGGTGGTGATGTTAGTGGGAGCCGGGA TTGGAGTCACGCCCTTTGCCTCCATTCTCAAGTCTGTCTGGTACAAATATTGTGATAATGCCACCAGTCT GAAACTCAAAAAGATCTACTTCTACTGGCTGTGCAGGGACACGCATGCCTTTGAGTGGTTTGCAGACCTG CTGCAGCTGCTGGAGACCCAGATGCAGGAAAGGAACAATGCCAACTTCCTCAGCTACAATATCTACCTTA CTGGCTGGGATGAATCTCAGGCCAATCACTTTGCTGTGCACCATGATGAGGAGAAAGATGTGATCACAGG CCTAAAACAGAAGACTCTGTATGGACGGCCCAACTGGGATAACGAGTTCAAGACCATTGCAAGTGAACAC CCTAACACCACAATAGGCGTTTTCCTGTGTGGCCCTGAAGCCTTGGCTGAAACTCTCAGTAAACAGAGCA TCTCCAACTCAGAATCCGGCCCGCGTGGAGTCCACTTCATTTTCAACAAGGAAAACTTCTAACTCCGCTC TTTCACCAGGAAACCAGTGTATGCTGCCAAATGTCCCAGTAATGCTGGTTGGTTGTAGGAGAGAAGGAGG AAAGGAAATCACAATGACACAGGCCCCCTAAAAGGAATTCCAAAGATCCTTACTAGTCATCTGTCATATT GCATGGAACTTCATGGTTTCAGAGCACTTTTTACATACATAGTTATGCATTGTTTCACATTTCCCTTTGA CATGCCAGAAGAGGGTAAGGCAAAAGCATTTTCTGGGACTCTCTCTAGGATAAACAATAGGATCTCAAAT GAGTTCCACACCTTCCTTCCAATTCAGAAGTTGAAACCACACCTAAGCCATCTGACTCTGGGTGTGGTCA CCTTTTCACCTTTAATACCAGGTTGTCTCAAGCCTCAGTTTTTATACTATCCACCAACAAAAAAGCAAGT CAAAGAAGAGCTCATCATTCTTAGCTGTTGTTTTCTCTTTAACCTTAGCATCATGCTAAAGGAAACTTTT TAAATAGAGGAGATAGTGATGCTGATGGTGACTTAATACTTCCTAAATGACAGTATCGGCACAGAAGTAT ATTCCAGTTTTATCAGCACAACAGTTTAACTGGGTCAGAGATTATTCCTAGTAAGAAATATTAGACTGAT TGGAGTTAAAGTAGCACTCTACATTTACCATGGTGTTTGGCAGAATTGGTGTTCAAGGTCAGTTTATTGA ATGAATGAATGAATGATTGAACAAATAGATGCAAACATTCAGAACTATATCATAATGGAATGTATGGCCA GAGACAGAATTACCCTTCTGTTTCTTTTACACACCAAAGTATCCTGATTCCCCTGGGAGTAGAGAGAAAG ATATGAACCAATAGGGAGAGTCAGGCCATTCTACTGGTATTCATCCAACTGTCATTTGAAGGGAGGAGGC ATGAATTCAGAGACCTATTCTCCTATGATGATGGTGGCTAACACTTCTAGTTCCAGTCTGTAAGCTGAAA AAGAAGAACACATTTATTTACTGACAGAAATGATCTGCTCCAGCCAAACTTTGAACTGAATTCTAAGAAA TGTATACATGATAATACATATCCTGCTTAGAATCCATTTCTTCTTTGGCTTAAATACCTATACAAATAGA TCTTATGTGCATAGACAATAACTTGTTTTTTTTTTTTTAATTGCAGCATTTTCATCACCACTAGTACCAG CATCACCATCTTCCTAAAATATAAATTTTAGAAACTGTAATCCCCAAATAACTGGGTTAAGAGTAAAATC ACATCCGATATTTTATGGGCATCCAGAAATGCAAGACAGAGTCTAGATGACTGCTAGACTAGTCACATGT GAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007807
• Insert Size: 1713 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC071229, AAH71229
• RefSeq Size: 3098 bp
• RefSeq ORF: 1713 bp
• Locus ID: 13058
• UniProt ID: Q61093
• Cytogenetics: X A1.1
• Gene Summary: This gene encodes the heavy chain component of a heterodimeric transmembrane ion transporter composed of both a heavy and a light chain. This transporter mediates the transfer of electrons from nicotinamide adenine dinucleotide phosphate (NADPH) to oxygen to generate superoxide. This reaction is important in the innate immune response to pathogens. However, increased activity of the encoded protein also leads to the generation of reactive oxygen species that result in oxidative stress and can cause tissue damage. Conversely, loss of function of the related gene in human causes chronic granulomatous disease. Alternative splicing results in multiple transcript variants, although the full-length nature of some of these variants has not been determined. [provided by RefSeq, May 2013]