Zwint (NM_025635) Mouse Untagged Clone

SKU MC204314

Zwint (untagged) - Mouse ZW10 interactor (Zwint), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Zwint
• Synonyms: 2010007E07Rik; 2600001N01Rik; D10Ertd749e; Zwint-1
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC027100; GAATCCAAAGGCGTGGTGAGCGACAGAACGATGGCGGACGCGGAGAAAAACGCTGTTGCGGAGAAAAACA ACGCTGTTGCGACGAAAGAGGTTCTGGCTGAGGCAGCAGCCATTCTAGAACCTGTAGGCTTGCAGGAAGA AGCAGAGCTGCCTGCCAAGATCATGGAGGAGTTTATGAGGAACTCGCGGAAGAAAGACAAACTGCTCTGT AGCCAGCTTCAAGTAGTGAACTTCTTACAGACTTTTCTGGCTCAGGAGGACACTGAGCAGAGCCCTGATG CTCTGGCTTCTGAAGATGCCAGTCGGCAAAAGGCAACTGAAACTAAGGAGCAGTGGAAGGACATGAAGGC CACATACATGGATCACGTGGATGTCATAAAATGTGCCCTGTCTGAGGCCTTACCCCAGGTTAAAGAGGCT CACAGGAAGTACACAGAGCTTCAGAAGGCTTTTGAGCAACTCGAGGCTAAGAAGCGAGTCCTTGAGGAGA AACTTCAGCTGGCTCAGAAGCAGTGGGTACTGCAACAGAAGCGTTTGCAGAACCTGACAAAGATTTCTGC AGAGGTCAAGAGGCGCCGGAAGAGGGCTCTGGAGAAGCTTGACGGATCCCATCAGGAACTGGAAACCTTG AAGCAGCAAGCGGGGCAGGAACAGGAGAAGCTGCAGAGGAACCAGAGCTACCTCCAGCTGCTGTGTTCGC TGCAGAATAAGCTGGTCATCTCTGAGGGCAAGGCTGAGGACAAAGATGTGAAAGGGCGAGCCCTTACAGC CAAATCCAAATCTCCTTAAAGATGAAAGGATGAGAAGAGAGCCGTATTCATACCCTGCTTGGGCTGAGAC CAGATTGGTTCCTTCACTGTTGTATCTCTAGGCCTAAGCCTGGAAGTTTGTTGCCTCTGTACAATCTAAT TCTCCAAGCTGATTCAATCTGGCATCTCTTGGCTTTTCAGTGAACTGTTGTGTTTTGTCTCATGCTAACT TTGACAGTATATTCTGGCTTGTTCTGTCTTCACCTGTGTCTAACTTGCTGTCCTTGCAACCTGTCTCTGT AAAAGTGTACCAGTGAAACTCACACGCATGCATGCACGCACACAGGCACGCATGCCACAACCACCCTTTT CTCTCGCTGATCTTCAGTAGTCTCTCCTCTTGTCCTCTCTTTTTCTTTGTCTTCTCTCCCTACCAGATGT GAAAGCAACTTTATGCGCCTGCTGTAATTGTTGTCTGAGGATTACTGCGAGTAAGCTCACCCTTTGTAGT TACTAAACTGGCCGGCTGGCTGGGTCAGCTCCGCTGTACTGGTTCATAACCCGGCTTCAAGTTGACTGAT TCAAAGGGGCCTCTCTTGGCTTCTGACTGAACTGCTCTGCTTGGAAAGTCTGATGGACTTCAGGAACAGA ACTGTACTGACTGCCCTGACTCAGCTGAACTCAACTACACCAAATTCATTGGTTCTCTCTGCTGTACTGC TCTTAGGTAGCCTCTCTCCTGTGCTGTCCTCCTGAGTCGTGCTGTCAAATCTCTCTCTGATTCATCACTT TGCCCCTCAATTAGACTGGCAGTTTCAAACACGGCACTTAACATTTCAGTTGTAGTCACTTGATACTGCA TTCCGAACTTGAGTCTATTGAATCCAGAATGTGAATCCATGCCTTTGTGACTGTGACCTCACTCTCTTCA GTATTGTGTAAAAGTTTTAGGAACTCTCCAAGTAAATACTTGGTAATTGCTCATTGATAGAACAGAAAAA TGCTGTGTAGATAATAAATAAACATATAACTATAAGATTAAAAAAAAAAAAAAAG
• Restriction Sites: RsrII-NotI
• ACCN: NM_025635
• Insert Size: 759 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC027100, AAH27100
• RefSeq Size: 1805 bp
• RefSeq ORF: 759 bp
• Locus ID: 52696
• UniProt ID: Q9CQU5
• Cytogenetics: 10 37.15 cM
• Summary: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase (By similarity).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein.