D2hgdh (NM_178882) Mouse Untagged Clone

SKU MC204094

D2hgdh (untagged) - Mouse D-2-hydroxyglutarate dehydrogenase (D2hgdh), nuclear gene encoding mitochondrial protein, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: D2hgdh
• Synonyms: AA408776; AA408778; AI325464
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC023277; AGGGGTGGGAAGCAGGGTGCGGGTGCGGGTGCGGACTCGGTCTTTGCACTCGGACGGAAGCTAGGGAACA ATGGTGCTCCCTCTGGTGTCCAGATGGTCTGCAAGGGTCCTCTGGGCATCTCCAGGATGGAGGAGGACCT ACACCCAGAGAGCTTGTGTGGGGTTGAAGCGGCTGGGTTGCCCTCGGGGTGTGTACAGCCCCTTGGCTCA CAGAGCGTACTCCGTGGTAGCTGGCAGCCCAGAGGTGACGCTGACCCCTGAGCGATATCCGGTGCAACGG CTGCCGTTTTCTACTGTGTCCGAGGAGGACCTGGCTGCATTTGAATGCATCATACCTGGCAGAGTTATCA CAGATCCAGAGCAACTGCAGACATGCAACGTTGACTGGCTGAAGACCGTCCGGGGTTGTAGTAAGGTGTT GCTGAGACCCCAGACCTCAGAGGAAGTCTCCCAGATTCTTAGGCACTGCTACAAGAGGAACTTGGCTGTG AACCCACAGGGCGGGAACACTGGCATGGTGGGTGGCAGTGTCCCTGTCTTTGACGAAGTCATCCTGTCCA CTGCCCTCATGAATCAAGTCATCAGCTTTCACGATGTATCTGGAATCCTGGTTTGCCAGGCAGGCTGTGT GCTGGAGGAGCTGAGCAGGTACGTGCAGGAGCGGGACTTCATCATGCCACTGGACTTGGGAGCGAAGGGC AGCTGCCACATCGGGGGAAATGTGGCAACAAATGCTGGTGGCCTGCGATTCCTGAGATATGGCTCCCTTC GAGGGACTGTCCTGGGCCTGGAAGTGGTACTCGCTGATGGGACCATCCTGAACTGCCTGACATCTCTGAG GAAGGACAACACAGGCTATGACCTGAAGCAGATGTTCATTGGGTCAGAGGGCACCCTGGGAGTCATCACT GCTGTGTCCATTGTGTGCCCACCCAGGCCTAAGGCTGTGAATGTGGCTTTCCTTGGCTGCCCTGGGTTCG CTGAGGTTCTGCAGACCTTCCGCACCTGCAGGGGGATGCTGGGTGAGATCCTGTCTGCCTTTGAGTTCAT GGATACTGAATGCATGCAGTTGGTTGGACAGCACCTCCAGCTGACCAACCCAGTACAAGAGAGTCCATTC TATGTCCTGGTTGAGACCTCAGGCTCCAGTGCAGGACATGATGCTGAGAAGCTGACGAACGTCCTGGAGC AGGTGCTGAATTCTGGCCTGGTGACAGATGGCACTATGGCCACCGACCAGAGGAAAGTCCAGATGCTGTG GGCCTTGCGGGAGAGGATCACAGAGGCACTCAGCCGCGATGGCTATGTGTTCAAGTATGACTTATCTCTC CCTGTGGAGCGGCTCTACGACCTTGTGATCGACCTACGCACCCGCCTTGGCCCCCGTGCCAAGCACGTGG TGGGATATGGACACCTGGGGGATGGCAACCTTCACCTGAACGTGACAGCAGAGGCCTTTAGCCGGGAGTT GCTCGGTGCCTTGGAACCTTATGTGTATGCCTGGACAGCTGAGCAGCGGGGCAGTGTCAGTGCGGAGCAC GGCCTGGGCTTCAAGAAGAAGGATGTACTTGGCTATAGCAAGCCACCTGTGGCCGTGACGCTTATGCAAC AGCTCAAGGCCATGCTGGACCCCAAAGGCATCCTGAACCCCTATAAGACTCTACCTGCCCAGGCCTAAAT GAAGCTTTTCAGCTTGTGCAAGATGCTAGGACTAGGTGAAGGAAGGTTTGGTAGGCCTGGAGGCCTCCCA GCTGATGTCATCCACAATAGACCAGGTTACTGACTCTCACTGTTAGCCAGGAGCCAGCTCTGAGCACCCT CTGGACAAGGTTGCCTCTCTGTGGGGGCAAACAGATCAGATAGAGCTGCCAGGCCCTTCTTTTGTGTCCT GTGGACAGAATAAGTGGGTGGGCAATTGCCCTACTCCCAAACAGGCCAGATCTCACCAGCTCTGGGGTGG GTGGCCTGTGGAGACCTGCTTTGTTGCATCTCTATAGCGCGTAATTTTAAAAGATCCCTACTTGCCTGGT GACTTGCGAGGCTGCCACCAGTCTCTCACTCTCACTCAGCTTCGCTAATGGCCAGTTGGCCATTTTCCCC TGGAGCTGTGATTGCTCCCCCATCCCCTCATGGCTAGCTTTGTCAAACTGTCGCCAACAACTGCCCCAAT GGATAGAAAACACCAGTTTTATTATCTTAAAACTAGACAGATAACTCAATGGCTGGGTGAAGAATTTCCT GCCCCAATCTTATCAACTAGCTCCCTCAATCTTGCCTGGCCTTCTGCCCACAGCAGAAGCTATAAACTCT GTGAGCCCCAAGACCTACATGTCTACAGCATCCTACTCGCTCCAAAGCCTGGTTCAAATTCTGCTTTCTT CTTTCTTTCTTCTGCCTGGGACCTACAACTTCAATCTTTTTCCCTTCACCCAACAATTAGCTTCTGCCTC CTTTATTGACACAATCAAGAATCAATTAGGGAACCAATACCTCTCACTCCACATCTCTGGGAAGAGAACC ACGTGATCTCTTGGATGGATCTTACTGGACAGTTTGCCCTGTGGCATGTATTCTCTGAGGGCTGCAGCAG AGTTGTCTAGCCCCAGATTAGTTACCACAGTGACAGAGAATGCCAGAACTCATTCCCAAGGGCCTGAGTG GGGTTTGCTGTCATATCAAGCAGGCCTTGAATTGGTAACTGTGGTCCACCTGGCTAGGACATTCCCTCAG GTTTTGTTCGTTTTAAGACAGGTTCTCTCTACGTAATTCTTACTGTTCTGGAACTTATGATGTAGACCAG ATAGACCTTTAACTCACAGAGCTCCTCCATGCCTCTGCCTTTACTTCTGCCCCCTGAGCAATGTGATTAA AGGCAAGTGCCACTACACCCAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_178882
• Insert Size: 1608 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC023277, AAH23277
• RefSeq Size: 2908 bp
• RefSeq ORF: 1608 bp
• Locus ID: 98314
• UniProt ID: Q8CIM3
• Cytogenetics: 1 D
• Summary: Catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate splice junction at the 3' end of the first exon compared to variant 1, which results in translation initiation from a downstream AUG. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from transcript and genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.