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B4galt1 (NM_022305) Mouse Untagged Clone

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SKU
MC203647
B4galt1 (untagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1), (10ug)
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ORF Plasmid:
Tag Free tGFP DDK
  $457.00
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Specifications
Specifications
Product Data
Type Mouse Untagged Clone
Target Symbol B4galt1
Synonyms 4-GalT; 4-GalT1; B-1; b4Gal-T1; beta-1; beta4Gal-T1; Ga; GalT; Ggtb; Ggtb2
Vector PCMV6-Kan/Neo
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
Fully Sequenced ORF
>BC053006
GCTGATCTCTCCAAAGTCCCGCGGCCAGGGGTGTCGGCTTTCCGCCGCAGGCGGCCGGTGCTGCGCTAGG TCCTAGCCCCCTCCCCCCGGCCCCTCCTTCGATCGCTGTGGTCGGGTAGCGCCTGGCGGGCGGCCTGCGG GCGGGCCGTCCTCTCAGCCGTAGCCCACCCCCTCTTAAAGCCGCGGCGGGAAGATGAGGTTTCGTGAGCA GTTCCTGGGCGGCAGCGCCGCGATGCCGGGCGCGACCCTGCAGCGGGCCTGCCGCCTGCTCGTGGCCGTC TGCGCGCTGCACCTCGGCGTCACCCTCGTCTATTACCTCTCTGGCCGCGATCTGAGCCGCCTGCCCCAGT TGGTCGGAGTCTCCTCTACACTGCAGGGCGGCACGAACGGCGCCGCAGCCAGCAAGCAGCCCCCAGGAGA GCAGCGGCCGCGGGGTGCGCGGCCGCCGCCTCCTTTAGGCGTCTCCCCGAAGCCTCGCCCGGGTCTCGAC TCCAGCCCTGGTGCAGCTTCTGGCCCCGGCTTGAAGAGCAACTTGTCTTCGTTGCCAGTGCCCACCACCA CTGGACTGTTGTCGCTGCCAGCTTGCCCTGAGGAGTCCCCGCTGCTCGTTGGCCCCATGCTGATTGACTT TAATATTGCTGTGGATCTGGAGCTTTTGGCAAAGAAGAACCCAGAGATAAAGACGGGCGGCCGTTACTCC CCCAAGGACTGTGTCTCTCCTCACAAGGTGGCCATCATCATCCCATTCCGTAACCGGCAGGAGCATCTCA AATACTGGCTGTATTATTTGCATCCCATCCTTCAGCGCCAGCAACTCGACTATGGCATCTACGTCATCAA TCAGGCTGGAGACACCATGTTCAATCGAGCTAAGCTGCTCAATATTGGCTTTCAAGAGGCCTTGAAGGAC TATGATTACAACTGCTTTGTGTTCAGTGATGTGGACCTCATTCCGATGGACGACCGTAATGCCTACAGGT GTTTTTCGCAGCCACGGCACATTTCTGTTGCAATGGACAAGTTCGGGTTTAGCCTGCCATATGTTCAGTA TTTTGGAGGTGTCTCTGCTCTCAGTAAACAACAGTTTCTTGCCATCAATGGATTCCCTAATAATTATTGG GGTTGGGGAGGAGAAGATGACGACATTTTTAACAGATTAGTTCATAAAGGCATGTCTATATCACGTCCAA ATGCTGTAGTAGGGAGGTGTCGAATGATCCGGCATTCAAGAGACAAGAAAAATGAGCCCAATCCTCAGAG GTTTGACCGGATCGCACATACAAAGGAAACGATGCGCTTCGATGGTTTGAACTCACTTACCTACAAGGTG TTGGATGTACAGAGATACCCGTTATATACCCAAATCACAGTGGACATCGGGACACCGAGATAGCATTTTT AGTACCAATAAGAGACCTGAGAATGGCCGGAGACCTCAGATATGTGTCTCTGCCAGTTGACTGGGCTGGT CCCTCTCATTTGTACAGTCTGAATGACAGTTCTTCTTATCATTCAGACGTCCCTCCAGATGCCCAGGGTG AGTGTAACATTTACCCACAACCTGGCTCGGCACTGGATGAAATTCTACAAGGTGAGTGGAGTGTAAAACT GGTCAGCCCTTGGAGAGACTTCTTGGTTGTGTCACCCCCAAAGAGTCAGAACTGTACACAGTTCAAAACT TAGTGACTGTGGGTCACATTCCCACTGTTGAAACTGCTAAATTGTGACCTGGGGAAGGACTTTGCTTTAG TCGGTGATGTTCGTACTTGGTGACAAATTGAGACTGCTGCTGGATTCAGATTGACAAGATTTTCTTGGAT TTTTTTTTTATACGAAAATCAAAATTTCAATCAGTCTCGTGCTCTGTCCCTTTACATCGGTATGCGACTA TTACAATCACTGTGTGTGTGTCTTTTCTTAGCAAAGGCGTTTTAAAACTTGAGCCTGGACCTTGGGGTCC TGTAGTGTGTGGATTCCAAGGCCTTGCCCTCAGAGCAGGGGCCTGGGCACTCTCACTCACGTGGCCTGTC TCCAGATCCCTGTCTGATTTCTGAATGTAAAGAGGCTTTTTGTTTTGTTTTTGTTTTTGTTTTTAGAAGC AGTTCGTAGTATTTGAAAGAATAAATCAAGTTTTGATTATGCTATAGGTTGATTTTTGTGTTGATCCAAA TCAGAATAGCTATTGAGTGTTTAAGTCATGACTTTATTTTTCTGGGCATGCTATATAAACTTGAATTTCC TATGTATTTTTATTGTGGTATTTTAAATGTGGGGGAAGGGATTGGGTATTTTTTAGGGGAAAAAAAATAA TGTATGCTGTAGTGGTCAAAGGAGCTCTGATTGAGCTGGCAGGCCAGGTTTTGTAAAGAGCAAAATTACT TTCAGGTCCCTTGTTGAGGGAACAGGTCAGCGTTATCCTGCCAGACATCTGGGTCAGGAACTTGGGAGAC AACATCTGCTTACCTGGCAGAACTCCCCACTCCACTCCCCCAGGTGGGCTGGGTGGAAGGACCCTCAGCC ACCAAGAACTGACGCCCTCATGGCCCTAGACTATGGGGTTGAAAACTCATAGCAGTCAGGTTTGTTTACC ATCTCTCCGAGCCTCCACCACACTCCCTGACCCACCCAACGTCTCCAGTTCTTATTGTTCTCCTTCCTTC CTTCTTTCCTTTCCCTTCTCACCTCTTTGCCTCCTTCCCTAAAATTCGCTGTGGCCCTCGGGGTCAGACT GTTCATTCTCCAGCATGATTGTGCCTTTTAATTAGAGGTCTAGAAAGTTCACCTGAACCCACACCCCCTT CTTCCTGAGAATTCTGGTGCCTCTAAAGCCCCTCCTTGCCACTCAGGGTTTTAGTGGGTGCTCCCACTGC TCAGTGTACCCATGAGCTGTCGTCATTCACAGAGCCCTGTGCCAGGCCGTGTTCTGTGCCTGCCTGCGGT GATGTAGCCAGAAGCACGTACTCAGGTCATCAAGCCGAGAGGGCGTCAAAGAGGAAGCCTGGGCTGGGAT CCTTAGGCTCAGCTGTCTGTCTAATCCCAGAGCTAAGCCCTTTCTGCTTCGGGCAGCCTGGACTCCAGCC CTGAGTATAGAGGCGGTGTGGATGGCTGGCCCTCATCCTACCTGTGTCTTTATTGCTTTGATCACCACAG TCTCTAACTTGCTGCTTTCTCCAGAAGCCTTTTTATGTATGGGTTAGAAACTCTTTTTTCCCTAGTCAGA GCAGCCAGGCTGTACAAAATCTGTGCTCTCTCGGTTCCCCATGCCTCTAGAAGGCCGTTAAAACCAAGAC TGGATGCACTGAGAGGAGTCTTTTTTTTTTTTTTTTTTTTTTTCTGTCCCAAGATCCTGACTTTTCCAAA GTGCCTTAAAAGAAGGGAGACAGACTCATTGAGTGTGTTGCTTCTTTCTCTCCTAAAGCAAACCTCTGCC TTTTCTCTATGCTGTTCGTGTTGGGTTTTGTTCATGTCAGATACGTGGTTCATTCTCAGGACCAAGGGAA ACTGTCTTGCCTGTCCTCCTATCTCCTCCCTTTCAGCATCCTTTGGGGAACCTCCAGCCCAAGGCACCGT TAGGCTTGGGCATTAGCCTGGCCCAAGAGATGCCACTGGGGACTAGAGGACAGAAGGGGACGTTCCCTTC ACCTGGGAATTCTCACCCTGGTGTCAACATGCTGTGCCTTTCTCTTTCTCTATTTTTGTTGGTGACCTGG ATAGTGTGGCCTTTGGGACAGATCAGAGGCACAGGAAAGCAAAGGAAATGTGCCACCCTTTAGCCTGGCA GGGTCTCCCTGCCCACTGGGCTGTTCCAGACCTGTGATACTGTGTTTGTATGTTGATATCCCATGTGTAC AAATGTGTTTACCATTGGGGCTGGGGGTGGGTGGCTGTCTCTGACCTTGGCATTCAGAACAGACATCTGT ATTTTTGCCTTTGAAATGCAGTAATATAACGTGAATAAATGACCTTATCTTTGTAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Restriction Sites AscI-NotI |
ACCN NM_022305
Insert Size 1200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq BC053006, AAH53006
RefSeq Size 4009 bp
RefSeq ORF 1200 bp
Locus ID 14595
UniProt ID P15535
Cytogenetics 4 20.46 cM
Summary This gene encodes two distinct enzyme isoforms, a long membrane-bound form and a short soluble form. These alternate isoforms are thought to be produced through alternative nested transcription initiation and different in-frame start codon usage. These enzymes catalyze the transfer of galactose to acceptor sugars, such as N-acetylglucosamine and glucose. The long form of this enzyme is localized to the trans-Golgi membrane and is involved in glycoconjugate biosynthesis. The short form functions in lactose biosynthesis though formation of a heterodimer with alpha-lactalbumin. provided by RefSeq, Nov 2012
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Resources
"NM_022305" in other vectors (4)
SKU Description Size Price
MG206270 B4galt1 (tGFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1) 10 ug
$886.00
MR206270 B4galt1 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1) 10 ug
$686.00
MR206270L3 Lenti ORF clone of B4galt1 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1) 10 ug
$986.00
MR206270L4 Lenti ORF clone of B4galt1 (mGFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 (B4galt1) 10 ug
$986.00

file-alt Citations

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