Atg7 (NM_028835) Mouse Untagged Clone

SKU MC203326

Atg7 (untagged) - Mouse autophagy-related 7 (yeast) (Atg7), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Atg7
• Synonyms: 1810013K23Rik; Agp7; Apg7l; Atg7l; Gm21553
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC058597; GGAAGTTGAGCGGCGGCTGGTAAGAACAGTAGCCATGGGGGACCCTGGACTGGCCAAGTTGCAGTTCGCC CCCTTTAATAGTGCCCTGGACGTTGGCTTCTGGCACGAACTGACCCAGAAGAAGTTGAACGAGTACCGCC TGGACGAGGCACCCAAAGACATCAAGGGCTATTACTACAATGGTGACTCTGCTGGTCTGCCCACCCGCTT GACGTTGGAGTTCAGTGCTTTTGACATGAGTGCCTCCACGCCTGCCCACTGCTGCCCGGCCATGGGAACC CTGCACAACACCAACACACTTGAGGCTTTTAAGACAGCAGACAAGAAGCTCCTTCTGGAGCAGTCAGCAA ATGAGATCTGGGAAGCCATAAAGTCAGGTGCTGCTCTCGAAAACCCCATGCTCCTCAACAAGTTTCTGCT CCTGACCTTCGCGGACCTAAAGAAGTACCACTTCTACTACTGGTTTTGCTGCCCCGCCCTCTGTCTTCCT GAGAGCATCCCTCTAATCCGGGGACCTGTGAGCTTGGATCAAAGGCTTTCACCAAAACAGATCCAGGCCC TGGAGCATGCCTATGATGATCTGTGTCGAGCCGAAGGCGTCACGGCCCTGCCCTACTTCTTATTCAAGTA CGATGACGACACTGTTCTGGTCTCCTTGCTCAAACACTACAGTGATTTCTTCCAAGGTCAAAGGACAAAG ATAACAGTTGGTGTGTACGATCCCTGTAACCTAGCCCAGTACCCTGGATGGCCTTTGAGGAATTTTTTGG TCCTGGCAGCCCACAGATGGAGCGGCAGTTTCCAGTCCGTTGAAGTCCTCTGCTTTCGGGACCGCACCAT GCAGGGAGCTAGAGACGTGACACATAGCATCATCTTTGAAGTGAAACTTCCAGAAATGGCATTTAGCCCA GATTGTCCTAAAGCTGTTGGCTGGGAGAAGAACCAGAAAGGAGGCATGGGTCCGAGGATGGTGAACCTCA GTGGATGTATGGACCCCAAAAGGCTGGCTGAGTCATCTGTGGATCTGAATCTCAAGCTGATGTGCTGGCG ATTGGTCCCCACCTTGGACTTGGACAAGGTCGTGTCTGTCAAGTGCCTGCTGCTGGGAGCTGGTACCTTG GGGTGTAATGTGGCTAGGACACTGATGGGCTGGGGCGTCAGACATGTCACCTTTGTGGATAACGCCAAGA TCTCCTACTCCAATCCCGTGAGGCAGCCTCTGTATGAATTTGAAGATTGTCTAGGGGGTGGCAAGCCCAA GGCCCTGGCTGCAGCAGAGCGGCTACAGAAAATATTTCCCGGAGTGAATGCCAGAGGGTTCAACATGAGC ATCCCCATGCCAGGACACCCTGTGAACTTCTCTGACGTCACGATGGAGCAGGCCCGCAGAGATGTGGAGC AGCTGGAGCAGCTCATTGATAACCATGATGTCATCTTCCTGCTAATGGACACCAGGGAGAGCCGGTGGCT TCCTACTGTTATTGCAGCCAGCAAGCGAAAGCTGGTCATCAACGCTGCCTTGGGGTTTGATACCTTTGTT GTCATGAGACATGGCCTGAAGAAACCCAAGCAGCAGGGAGCCGGAGACCTCTGCCCAAGCCATCTTGTAG CACCTGCTGACCTGGGCTCCTCACTTTTTGCCAACATCCCTGGATACAAGCTTGGCTGCTACTTCTGCAA TGATGTGGTGGCTCCAGGAGATTCAACCAGAGACCGGACTCTGGACCAGCAGTGCACAGTGAGCCGCCCA GGCCTGGCCGTGATTGCAGGTGCCCTGGCTGTGGAGCTGATGGTCTCTGTCCTGCAGCATCCTGAGGGGG GCTACGCCATCGCCAGCAGCAGTGATGACCGCATGAATGAGCCTCCCACCTCGCTGGGACTTGTGCCTCA CCAGATCCGGGGTTTTCTGTCACGGTTCGATAATGTTCTTCCTGTCAGCCTGGCATTTGATAAATGTACA GCCTGTTCACCCAAAGTTCTTGATCAGTACGAGCGAGAAGGATTCACCTTCCTAGCGAAGGTTTTTAACT CCTCACATTCCTTCTTAGAAGACTTGACCGGTCTTACCCTGCTCCATCAAGAGACCCAAGCTGCTGAGAT CTGGGACATGAGTGACGAGGAGACTGTCTGAAGCAAGCAACCACAGCTCAGGAGTACCTGGCCCTCAGCG CAGGACTGGACCGCAGGACTGGTGATCTGGGCCCTGCCACCTCCCTGGTCCTGATCTCCACATCTCCAAG GACGAGGGTGTACCCTCTGCCACCCAGTTGCACCCTTTCCTGTGCCATCTCACCAGCTCTGAACTCAATA ATAACCTTGGCATTGCCACTGATCTGGGGCTCAGGTCCTTCCATGTGCACTAATCTCCCCCCCCCCACAC ACACACTGTTGCTGAAGGACACCCCAGGACCCAACATAGATCAGACAAGGCTGTGCTAGGAGCCTCACCG GTAGGGCACCTGCTCTGGGCCCTGGGTAGCAGTGAGTGCTGAGTTTGTAGCCTCAAGTGTTCAAGTGGCA CACCAAGCCACCCTCCCCCAGCTGTGGGCATGCTGTGTGCCACCCTGTTCCAGGGATGGGAGAAGCTCCT GCCACAGCCCTGTACTGAAAAGCAGGGAAGAGCTCTGTAGGATGGGTGTGTCCAGCTGGGCCTAGTCAGG TGCCCTCACTCACGGGGTTGCTCCTGGGGCAAGGCTTGTCTTCCTCTTCACTCTGGGTGGGCCCTTGGCA GCTGTGGCCACCCATCCTAAATAGATGAGCTGCTCCCCTCCCACACCTGTGCACCTTCACTGGGGTCTCA GGTCCAGAACAGAAGCCCATGCACGGCTGGCTTAGCAGGTCTCAGGAAGGGAGACTAGAGAGGACCTTGG CCTAACACAGATGCTGCAACAAGCGGCCCTACCATCTGTGCAAGGCTCCCCACAAGTAGCCAGGCCTACC TGGGCACAGGGCCCCACAGCCCACATGCCACCCTAGGAGTCAAGAGCCACACAGCCTCGGTTTAAGAGCA CTTTATTATTGTTCTTAAGGCTACTTTTAAGTACAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_028835
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC058597, AAH58597
• RefSeq Size: 3059 bp
• RefSeq ORF: 2097 bp
• Locus ID: 74244
• UniProt ID: Q9D906
• Cytogenetics: 6 E3
• Summary: This gene encodes an E1-like activating enzyme that is essential for autophagy and cytoplasmic to vacuole transport. The encoded protein is also thought to modulate p53-dependent cell cycle pathways during prolonged metabolic stress. It has been associated with multiple functions, including axon membrane trafficking, axonal homeostasis, mitophagy, adipose differentiation, and hematopoietic stem cell maintenance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]; Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region, resulting in the initiation of translation from a downstream start codon, compared to variant 1. Variants 2 and 3 encode the same isoform (2), which has a shorter N-terminus, compared to isoform 1.