Gcgr (NM_008101) Mouse Untagged Clone

SKU MC203290

Gcgr (untagged) - Mouse glucagon receptor (Gcgr), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gcgr
• Synonyms: GR
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057988; CGCGAGGAGCGCAGCCCTAGCCCCGGCGACTGAGCACACCTGAGGAGAGGTGCACACACTCTGAGGACCT AGGTGTGCAACCTCTGCCAGATGTGGGGCGTGGCTACCCAGAGGCATGCCCCTCACCCAGCTCCACTGTC CCCACCTGCTGCTGCTGCTGTTGGTGCTGTCATGTCTGCCAGAGGCACCCTCTGCCCAGGTAATGGACTT TTTGTTTGAGAAGTGGAAGCTCTATAGTGACCAATGCCACCACAACCTAAGCCTGCTGCCCCCACCTACT GAGCTGGTCTGTAACAGAACCTTCGACAAGTACTCCTGCTGGCCTGACACCCCTCCCAACACCACTGCCA ACATTTCCTGCCCCTGGTACCTACCTTGGTACCACAAAGTGCAGCACCGCCTAGTGTTCAAGAGGTGTGG GCCCGATGGGCAGTGGGTTCGAGGGCCACGGGGGCAGCCGTGGCGCAACGCCTCCCAATGTCAGTTGGAT GATGAAGAGATCGAGGTCCAGAAGGGGGTGGCCAAGATGTATAGCAGCCAGCAGGTGATGTACACCGTGG GCTACAGTCTGTCCCTGGGGGCCTTGCTCCTTGCGCTGGTCATCCTGCTGGGCCTCAGGAAGCTGCACTG CACCCGAAACTACATCCATGGGAACCTGTTTGCGTCCTTTGTGCTCAAGGCTGGCTCTGTGTTGGTCATC GATTGGCTGCTGAAGACACGGTACAGCCAGAAGATTGGCGATGACCTCAGTGTGAGCGTCTGGCTCAGTG ACGGGGCGATGGCCGGCTGCAGAGTGGCCACAGTGATCATGCAGTACGGCATCATAGCCAACTATTGCTG GTTGCTGGTAGAGGGCGTGTACCTGTACAGCCTGCTGAGCCTTGCCACCTTCTCTGAGAGGAGCTTCTTT TCCCTCTACCTGGGCATTGGCTGGGGTGCGCCCCTGCTGTTTGTCATCCCCTGGGTGGTGGTCAAGTGTC TGTTTGAGAATGTTCAGTGCTGGACCAGCAATGACAACATGGGATTCTGGTGGATCCTGCGTATTCCTGT CTTCCTGGCCTTACTGATCAATTTTTTCATCTTTGTCCACATCATTCACCTTCTTGTGGCCAAGCTGCGT GCCCATCAGATGCACTATGCTGACTATAAGTTCCGGCTGGCCAGGTCCACGCTGACCCTCATCCCTCTGC TGGGGGTCCACGAGGTGGTCTTTGCCTTTGTGACTGACGAGCATGCCCAAGGCACCCTGCGCTCCACCAA GCTCTTTTTTGACCTGTTCCTCAGCTCCTTCCAGGGTCTGCTGGTGGCTGTTCTCTACTGTTTCCTCAAC AAGGAGGTGCAGGCAGAGCTGATGCGGCGTTGGAGGCAATGGCAAGAAGGCAAAGCTCTTCAGGAGGAAA GGTTGGCCAGCAGCCATGGCAGCCACATGGCCCCAGCAGGGCCTTGTCATGGTGATCCCTGTGAGAAACT TCAGCTTATGAGTGCAGGCAGCAGCAGTGGGACTGGCTGTGTGCCCTCTATGGAGACCTCGCTGGCCAGT AGTCTCCCAAGGTTGGCTGACAGCCCCACCTGAATCTCCACTGGAGCCTAGCCAGGCTGCGTTCAGAAAG GGCCTCAGAGGACAACCCAGAGCCAGATGCCCGGCCAAGGCTGAAGAGACAAAGCAGCAAGACAGCAGCT TGTACTGTGCACACTCCCCTAACCTGTCCTAGCCTGGCACAGGCCACAGTGACAGAGTAGGGGTTGGATA TGATGGAGAAGCCATGTTATCTATGAACTCTGAGTGTTCCCATGTGTGTTGACATGGTCCCTGTACCCAG ATATGTCCTTCAGTAAAAAGCTCGAGTGGGAGCTGCTGCACAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008101
• Insert Size: 1458 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC057988, AAH57988
• RefSeq Size: 1880 bp
• RefSeq ORF: 1458 bp
• Locus ID: 14527
• UniProt ID: Q61606
• Cytogenetics: 11 E2
• Summary: G-protein coupled receptor for glucagon that plays a central role in the regulation of blood glucose levels and glucose homeostasis. Regulates the rate of hepatic glucose production by promoting glycogen hydrolysis and gluconeogenesis. Plays an important role in mediating the responses to fasting. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Promotes activation of adenylate cyclase. Besides, plays a role in signaling via a phosphatidylinositol-calcium second messenger system.[UniProtKB/Swiss-Prot Function]