Kcnj15 (NM_019664) Mouse Untagged Clone

SKU MC203285

Kcnj15 (untagged) - Mouse potassium inwardly-rectifying channel, subfamily J, member 15 (Kcnj15), transcript variant 2, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Kcnj15
• Synonyms: 4930414N08Rik; AI182284; AI267127; IRKK; Kir4.2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC057915; CTGGGGAGTCCTTGCCATTCCAAGCCGCCCTGTGCCGTGAACAGTAAGACTGGTTCTTCACTTTGAAGAA CCCTCAGCACCTACCTTTGCAAGGCACCCTGGATCACTTCCTTGCCTGGTCTTAATTTCCCACCCACCCA GGAGCCTGCACTGGGCACTCGGCAGAAAGAAAGAGCACAGTGGGTTTCTGAAGTAGTCAGAGAGTGGAAG CAGTCTGACTGGAGAAACGGCTTTGTTCACGGCCCATGGAGAACACTGCTTTCCCCAGTGCCATAGCAGA GCCCCATGGTAGCCAGGTGGGAGAAGGGGAGCGAGGACGCGCCACTTACCCTGCAGAAGATTCCTGACCT ACAGAGTGAGTGACCAGGTGGTCCAAAGATGGATGCCATTCACCTTGGCATGTCCAGTGCCCCACTGGTG AAGCATACCAACGGGGTTGGACTCAAGGCCCACAGACCCCGAGTCATGTCAAAGAGTGGGCACAGTAATG TGAGAATCGATAAGGTAGACGGAATCTATTTACTCTACCTCCAGGACTTGTGGACAACCGTCATCGACAT GAAGTGGCGATACAAGCTCACCCTATTTGCTGCCACCTTTGTGATGACCTGGTTTCTGTTTGGAGTGGTC TACTATGCCATAGCCTTTATTCATGGTGACTTACAACTTGGGGAATCTAATTCCAACCACACACCCTGCA TTATGAAAGTGGACTCTCTCACAGGAGCATTCCTCTTTTCCTTGGAATCTCAGACAACCATTGGCTACGG GGTCCGTTCCATCACAGAGGAGTGTCCCCATGCTATCTTCCTCTTAGTCGCCCAACTGGTCATCACCACA TTGATTGAGATCTTCATTACGGGGACCTTTCTGGCTAAAATTGCAAGACCCAAAAAGCGAGCCGAGACCA TTAAGTTCAGCCACTGTGCTGTCATCAGCAAGCAGAATGGAAAGCTATGCCTGGTCATCCAGGTGGCCAA CATGAGGAAGAGTCTCCTGATTCAGTGCCAGCTCTCTGGAAAACTCCTGCAGACACACGTCACCAAAGAG GGAGAACGCATTCTCCTCAACCAGGCCACTGTCAAATTCCACGTGGACTCCTCTTCCGAGAGTCCCTTCC TCATCCTGCCCATGACCTTCTACCACGTGTTGGATGAGACAAGCCCCCTGCGGGACCTCACACCCCAAAA CCTAAAGGAGAAGGAGTTTGAGCTGGTGGTACTTCTCAACGCCACGGTGGAGTCTACCAGCGCCGTCTGC CAGAGCCGAACGTCTTACATCCCGGAGGAGATCTACTGGGGCTTTGAGTTTGTGCCTGTGGTTTCTCTCT CCAAAAATGGAAAGTATGTGGCTGATTTCAGTCAATTTGAGCAGATCAGGAAGAGCCCGGATTGTACCTT CTACTGTGCCGATTCTGAGAAGCAGAAGCTTGAAGAACAGTACAGGCAAGAGGACCAGAGGGAGCGGGAG CTGAGGAGCCTCCTGCTACAGCAGAGCAATGTCTGACTCCACCGGCCTCTGGGCGTCTCCTAACCAGGGC TCCTTTCAAAAGCAATGGCTGCCATGAACGTGGGATGGGTGGCCCCACTACCAAATCACACAGACGTTCG TGGCCATCTTCCCCTTTGTTCTGATGGCTGAACAAGCATTTCTGAATATAAAAAGATGTCTTCAGAGTGC CTGGTCAGAGGTGAGCACTCAAATTCAGATTTCCCCCCACCCCTAAAATGGAGCTATACATTGAAGAACT GAGTGTAGGAAGATGGAGAGAATTCCCGCTGGAGGGCAGACCCAGCAGTGCTGACAGTGGATGGCATGTA TGCCTACTCAAGAACATCTGCTCATAATGCAAAATATTTATTTACCCACCTAACAAGGGCATTGGTGGCT GAGGTCTGAGGCGTAATTTGCTATTTGCTCATAGCACTGCTTAAGAACTCACTCCTTCTCATGTTCAAGT GCATACGTTTTTATTTGCAATGACGGTTATCTGGAGATGACAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019664
• Insert Size: 1128 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC057915, AAH57915
• RefSeq Size: 2017 bp
• RefSeq ORF: 1128 bp
• Locus ID: 16516
• UniProt ID: O88932
• Cytogenetics: 16 55.86 cM
• Summary: Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region and uses a downstream start codon, compared to variant 1. The encoded isoform (b) has a shorter N-terminus, compared to isoform a. Variants 2, 3, 5, 7, 9 and 11 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.