Atp6v1a (NM_007508) Mouse Untagged Clone

SKU MC202829

Atp6v1a (untagged) - Mouse ATPase, H+ transporting, lysosomal V1 subunit A (Atp6v1a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Atp6v1a
• Synonyms: AI647066; Atp6a1; Atp6a2; Atp6v1a1; VA68; VPP2
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC038392 sequence for NM_007508; GTCTAGGTACACTTTCTGCATTTCGAGCCCGGGCAGGTAAATTTAACACAATGGATTTCTCCAAGCTACC CAAAATCCGCGATGAGGATAAAGAAAGTACATTTGGTTATGTGCATGGAGTCTCAGGGCCTGTGGTTACA GCCTGTGACATGGCGGGCGCTGCCATGTACGAGCTGGTGAGAGTGGGGCACAGCGAGCTGGTTGGAGAAA TTATTCGATTGGAAGGTGACATGGCCACCATTCAGGTGTATGAAGAGACTTCTGGTGTCTCTGTTGGAGA CCCCGTACTCCGCACTGGTAAACCTCTCTCGGTCGAGCTGGGTCCCGGGATTATGGGAGCCATTTTTGAT GGTATACAGAGACCTCTGTCGGATATCAGCAGTCAGACCCAAAGTATCTACATCCCCAGAGGAGTCAATG TGTCTGCTCTCAGCAGAGATATCAAATGGGAGTTTATACCCAGCAAAAACCTACGGGTTGGTAGTCATAT CACTGGTGGAGACATTTATGGGATTGTCAATGAGAACTCCCTCATCAAACACAAAATCATGTTGCCCCCA CGTAACAGAGGAAGCGTGACTTACATCGCGCCGCCTGGGAATTATGATGCGTCCGATGTCGTCCTGGAGC TTGAGTTTGAAGGTGTGAAGGAGAAGTTCAGCATGGTCCAAGTGTGGCCTGTGCGGCAGGTGCGGCCTGT CACTGAGAAGCTGCCCGCCAATCACCCCTTGCTTACTGGCCAGAGAGTCCTCGATGCCCTTTTCCCGTGT GTTCAGGGAGGAACTACTGCTATCCCAGGCGCCTTTGGCTGTGGAAAGACTGTGATTTCCCAGTCTCTAT CCAAGTACTCCAACAGTGACGTCATCATCTATGTCGGCTGCGGTGAGAGAGGCAACGAGATGTCAGAAGT TCTCCGCGACTTCCCTGAGCTCACCATGGAGGTTGATGGGAAAGTAGAGTCCATCATGAAGAGGACAGCG CTGGTAGCCAACACCTCCAACATGCCTGTGGCTGCAAGAGAGGCCTCCATCTACACTGGAATTACACTAT CAGAATATTTCCGTGACATGGGCTACCACGTCAGTATGATGGCGGACTCTACCTCTAGATGGGCTGAGGC CCTCAGAGAAATCTCTGGTCGCTTAGCTGAGATGCCTGCAGATAGTGGATACCCTGCATACCTTGGTGCC CGGCTGGCTTCTTTCTATGAGCGAGCAGGCAGAGTGAAATGTCTTGGAAACCCTGAGAGAGAAGGGAGTG TCAGCATTGTAGGAGCAGTTTCTCCACCTGGTGGTGATTTTTCTGATCCAGTCACTTCTGCAACTCTGGG TATTGTTCAGGTGTTCTGGGGCTTGGATAAGAAGTTAGCTCAGCGCAAGCACTTCCCCTCTGTCAACTGG CTCATCAGCTACAGCAAGTACATGCGTGCCCTGGACGAGTACTATGACAAGCACTTCACGGAGTTTGTTC CTCTGAGGACCAAAGCTAAGGAGATCCTGCAGGAAGAAGAGGATCTGGCAGAAATCGTGCAGCTCGTGGG AAAGGCCTCTTTAGCAGAGACGGATAAAATCACTCTGGAGGTAGCAAAACTTATTAAAGATGACTTCCTA CAGCAAAATGGGTACACTCCTTATGACAGGTTCTGTCCATTCTACAAGACAGTGGGGATGCTGTCCAACA TGATTTCATTCTATGACATGGCCCGCCGAGCTGTGGAGACCACTGCCCAGAGTGACAATAAGATCACATG GTCCATTATCCGGGAGCACATGGGGGAGATTCTCTATAAACTCTCCTCCATGAAATTCAAGGATCCAGTG AAAGATGGCGAGGCAAAGATCAAGGCCGACTATGCACAGCTTCTTGAAGATATGCAGAATGCATTCCGTA GCCTTGAAGACTAGAACTGTGATTTCTTCCCTCTTCCACGGCAAGCTCATACGTGTATATTTTCCTGAAT TTCTCATCTCCAACCCTTAGCTTCCATATTGTGCAGCTTTGAGACTAGTGCCTATGTGTGTTCTTGTTCA TTTTGCTGTTTCTTTGGTAGGTCTTATAAAACAAACATTCCTTTGTTCCAGTGTTTGAAGGAGCTAGCTC CCTTACCTTTATCTGAAGTGATGAATGTAGTGCATATGATATACATTGTAAGATACACATTGTAACATGA TCCATACTGTAAACTTGTATGTAAGGTGACTACCCCTTCCCTCATCTCCAGTAAACTGTAAACAGGACTA CTGCATGTACTCTGTTGGGAATGGAAGGCCAGAACTCCATACCGTGGATGGATGGGTACTTAGGAAACAA CTCAGCATTTGTAGTCAGACCACTTGTAACTTAGTGGTTTGTTGAGTAACCATTTTGCAGGAAATACTTC CATTTAAAAACATAAAAGATTAATGTTCCAATTATTTGTATCAATCAGGACCATTTTTGTGGGGCACTTG GGAACTATTTGTTTTTCAAACAGACATTTGCAAGACTGAACATAATAGATAAATCAGTTACCTCTGAAAA TGTGAAAGAAAGGAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_007508
• Insert Size: 1854 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC038392, AAH38392
• RefSeq Size: 2548 bp
• RefSeq ORF: 1854 bp
• Locus ID: 11964
• UniProt ID: P50516
• Cytogenetics: 16 B4
• Summary: Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation. May play a role in neurite development and synaptic connectivity.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.