Mgrn1 (NM_029657) Mouse Untagged Clone

SKU MC202619

Mgrn1 (untagged) - Mouse mahogunin, ring finger 1 (Mgrn1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Mgrn1
• Synonyms: 2610042J20Rik; md; mKIAA0544; nc
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC046830 sequence for NM_029657; GGAGCGGCCGCAGCGGCGGCGGCGGCGGTGGCGGCGGTGGGTACGCGTTGGTGCGGCCTGTTTTGGGCCA TGTCCGTGTGAGGACGCCGCAGCCGACAACCTCTCCTCTCCGGCTGGCCGGTAGAAACGCGCACCATGGG CTCCATCCTGAGTCGCCGCATCGCGGGCGTAGAGGACATCGACATACAGGCCAACTCGGCCTATCGCTAC CCTCCGAAGTCCGGAAACTACTTTGCTTCGCATTTTTTCATGGGAGGAGAGAAATTTGACACTCCCCATC CTGAAGGTTACCTCTTTGGAGAGAACATGGACCTGAACTTCCTGGGCAGTCGTCCAGTCCAGTTTCCCTA TGTCACCCCTGCCCCCCATGAGCCAGTGAAGACACTGAGGAGCCTGGTGAACATTCGCAAAGACTCTCTC CGGCTTGTGAGGTACAAAGAAGACGCAGACAGCCCCACGGAGGACGGTGAAAAGCCCCGCGTGCTCTACA GTCTGGAATTCACCTTTGATGCCGATGCCCGTGTGGCTATCACCATCTATTGTCAGGCGGTAGAGGAGCT GGTGAATGGCGTGGCAGTGTACAGCTGTAAGAACCCTTCTCTGCAATCTGAGACCGTCCACTACAAGAGA GGCGTTAGCCAGCAGTTCTCCCTGCCTTCTTTCAAGATTGACTTCTCCGAGTGGAAGGATGATGAGCTGA ACTTTGACCTGGACCGGGGTGTGTTTCCAGTAGTCATCCAGGCTGTGGTGGACGAAGGGGATGTTGTGGA AGTGACTGGCCACGCTCATGTGCTCCTGGCTGCCTTTGAGAAGCATGTGGATGGCAGCTTCTCCGTGAAG CCATTAAAGCAGAAGCAAATTGTGGACCGGGTCAGCTACCTGCTGCAGGAGATCTACGGCATCGAGAACA AGAACAACCAGGAGACTAAGCCTTCTGATGATGAGAACAGTGACAACAGCAGTGAGTGTGTGGTGTGCCT GTCAGACCTGCGGGACACACTGATCCTGCCCTGCCGACACCTGTGCCTCTGCACTTCCTGTGCTGACACA CTGCGGTACCAGGCCAACAACTGCCCCATCTGCCGGCTGCCGTTCCGAGCCCTTCTGCAGATCCGGGCTG TGCGGAAGAAGCCAGGGGCCCTGTCTCCCATCTCCTTCAGCCCTGTCTTGGCCCAAAGTGTGGACCACGA TGAGCACTCTAGTTCTGACAGTATCCCACCTGGCTATGAGCCCATCTCCTTGCTTGAGGCACTCAATGGT CTACGAGCTGTCTCCCCGGCTATCCCATCAGCTCCCCTCTATGAGGAAATCACCTACTCAGGCATCTCGG ACGGTCTTTCCCAGGCCAGTTGTCCCCTTGCTGGACTTGATCGAATCATGGAAAGTGGCCTACAGAAGGG CAAGACACAGAGCAAGTCTCCAGACAGCACCCTGCGGTCTCCATCATTCCCCATCCATGAGGAGGACGAG GAGAAGCTCTCAGAGGACTCGGATGCTCCTCTCCCACCGAGCGGTGTGGAGCTGGTACTGCGGGAGAGCA GCTCCCCTGAGAGTTTCGGAACAGAGGAGGGAGATGAGCCGTCCCTAAAGCAAGGGAGCCGAGTACCCTC TATTGATGATGTCCTGCAAGATGGCAGTCCGCAGCATCATGGTTGCAGCCAGCCAGTCCCTCCTGCTGAC ATCTACCTACCAGCCCTGGGTCCTGAGTCGTGCTCTGTTGGTATAGAGGAGTAAGCTGGATGGTCCACCT CCATGGAGACGCCACACAGCCTTGGCACCACCAGCTCCCCCTGGCCTCTGCTGAGTGGCTCTAGTCCTGA GCCTGGAGTTGCTGAGCTGACCCCATTCTGAAAGTCTGGCTTAGCGGGCAACACAGAGCCCTCAGCATCC CAAACATTGCCAGGAGGCTTCCCTGGACCCCAGCGAGACCCTGGCCTCTCTTGTCTGCACGCCTCATGTG GCCACTGGGATTTGAGGGTCCACAGTGAACCTTGATCAAATTGCACAGTGGGTTGTTCCTCCACACACCC TCCTTTTTTTACGGTCAATATATTTGTAAATGGTACAAGATGAAGGACAACAACTGTCTCCATGGGCCAC GTGCCCTAGAAGGACTCTCCCCAACTGTCAAAGCTAATGCTTTGAGCACTTAGCAAACCAGCATAAGAGA ATCTACCCATGGAATGTTCTGGAAGGTGGGCAGTCCAGGTTACAGTTGATGAAGGAAACAGTGCCTGTCT GCCTCTCATGTGTGACCCCAAGGCAGCCAGGCTGTCACTGCTGCTGTCACAGGCTGACACCTTACAGATG TCCTGACCATGGGTGACCCTCCTCGGAGCGTCCAGATCCCCTCCTGCTCTGCTCTATTACCTACTAGTGG TCCCTGGCAGCTGTGGGCAAGGCCTGGGTAGTCAGACTGTTAACACCACAGTCCTTAGCAGTGGTCAATG TCCAGTTCCCCCGCACAGGTCCTGCTTCACTCACATCCTCACCCGCTGCCAAGGTGGCGTTTTGTGCTTG CAGGGCAGCACTGCCCACCACCTGCCAGGGAGGAGCCTGAGCAGCTTCCATAGCAGATCCGTTGCCAGTT ACCTGGCCTGTAAGCTTCTTACTGATTGATTGTCTAAGGCTCCTGTCAGAGCCCACCTAGAAGCAGGCTC CTAGTGATACTTTGTTTCCAAGGCAGAAGTGGCCCATGAGTGGCCCCAGAGCAGAGGTGCCCCAGGCAGT GCAGCTCCCTGGTGGGTTGGTAGAGGGACCTCAGCTTAAGGAACTGCCCTGCCACTCACCCAGGTGACAG CCTGTAGCAGTGCATGGAGACCCCTTTAGGCTCAGGGCTGCTCGAGCTCTGACTCTAACCCTGCTCCTTC CTAGCACAGCTCTGCACATCCATCTCTCTCTCTTGCTGAATGCACATATGTGCACACATGTCAGCATGTG CATGCTCACTCCCCCACTTCTCACCTGCCAACATCCTGGCCTCTGGCCGTGGCACTGGTGCCAGTACTTT GAAAGATGGGGTGCAGCCTACACACTTACCTGTCTCAGACTCACATGTACTATCATCTGATGTACTCTGA TTGGCTAGTTTGGGTTCATTTTTACTGTATATTTATAGTAATAAAATCATGCAGCAATAAAAAAAAAAAA AAAAAAAAAAAAAAA
• Restriction Sites: AscI-NotI
• ACCN: NM_029657
• Insert Size: 1599 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC046830, AAH46830
• RefSeq Size: 3165 bp
• RefSeq ORF: 1599 bp
• Locus ID: 17237
• UniProt ID: Q9D074
• Cytogenetics: 16 2.48 cM
• Summary: E3 ubiquitin-protein ligase. Mediates TSG101 monoubiquitination at multiple sites. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination (By similarity). Acts also as a negative regulator of hedgehog signaling (PubMed:29290584).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (2) uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.