Poc1a (NM_027354) Mouse Untagged Clone

SKU MC201416

Poc1a (untagged) - Mouse POC1 centriolar protein homolog A (Chlamydomonas) (Poc1a), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Poc1a
• Synonyms: 2510040D07Rik; cha; Wdr51a
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC034901 sequence for NM_027354; TGGGGCACAGCTGGTCCTACCAGCCCGCTAGCCCCACCATGGCTGCTCCCAGCCAGGAGGACCCCTCACT GGAGAGACATTTTAAGGGCCACCGAGATGCAGTCACCTGTGTGGACTTCAGTCTCAACACCAAGCACTTG GCCAGTGGCTCCATGGACTCAACCCTCATGATCTGGCACATGAAGTCCCAGTCACGTGCCTACCGCTTCA CGGGCCACAAGGACGCTGTGACCTGTGTGAATTTCTCCCCATCCGGACACCTTCTTGCCTCAGGCTCCCG AGACAAGACTGTTCGCATCTGGGTACCCAATGTCAAAGGTGAGTCAACTGTATTTCGTGCACACACGGCC ACAGTGAGGAGTGTCCACTTCTGCAGCGATGGCCAGTCACTTGTGACAGCCTCTGATGACAAGACAGTCA AAGTGTGGTCAACTCATCGCCAGAGATTTCTGTTTTCCCTGACCCAGCACATCAACTGGGTCCGCTGTGC CAAGTTCTCCCCTGACGGGCGGCTCATCGTGTCTGCCAGTGATGACAAGACTGTCAAGCTGTGGGACAAG ACCAGCCGGGAATGTATCCACTCATACTGTGAGCACGGCGGTTTTGTCACCTACGTGGACTTCCACCCCA GTGGTACCTGCATTGCTGCTGCCGGCATGGACAACACGGTGAAGGTGTGGGATGCGCGGACTCATCGGCT GTTGCAGCATTATCAGTTACACAGTGCAGCCGTGAATGCCCTCTCCTTCCACCCATCGGGAAACTACCTC ATCACAGCCTCCAGTGACTCAACGCTGAAGATCCTGGACCTGATGGAGGGCCGGCTGCTCTATACGCTCC ATGGGCACCAGGGGCCTGCTACCACCGTTGCCTTTTCAAGAACGGGGGAGTATTTCGCTTCTGGAGGCTC CGATGAGCAGGTGATGGTCTGGAAGAGTAACTTTGACATTGTTGATTATGGAGACATGAAGGCACGGAGG CCCCCACCCCTAACCAGCTCTTCTGGAACTCTGCCAAAAATGGATTTGCCTGTCCCACCAGGCAGAGACA GGAGTCTGGAGTCAGTTCAAGGCGAGCCCCAGGAGTCCATCAGCATGCCTCAGACACTGACCAGCACCCT GGAGCACATCGTGGGCCAGCTGGACATCCTCACGCAGACAGTGTCCATTCTGGAACAGCGACTGACACTA ACAGAAGACAGGCTGAAGCAGTGCCTGGAGAACCAGCAGTTAATCATGCAGAGAACGCCACCGTGATGAG GGGAGCAAGGATCAGGAGCTCAGTGATTGGGGGGACAGCAGGCCAGGGATTTGTATGGGGACTTGAGTAA ATAAATAAAGGGATCCCACCTGAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-NotI
• ACCN: NM_027354
• Insert Size: 1218 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC034901, AAH34901
• RefSeq Size: 1372 bp
• RefSeq ORF: 1218 bp
• Locus ID: 70235
• UniProt ID: Q8JZX3
• Cytogenetics: 9 F1
• Summary: Plays an important role in centriole assembly and/or stability and ciliogenesis. Involved in early steps of centriole duplication, as well as in the later steps of centriole length control. Acts in concert with POC1B to ensure centriole integrity and proper mitotic spindle formation (By similarity).[UniProtKB/Swiss-Prot Function]