Atp6v0d1 (NM_013477) Mouse Untagged Clone

SKU MC200877

Atp6v0d1 (untagged) - Mouse ATPase, H+ transporting, lysosomal V0 subunit D1 (Atp6v0d1), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Atp6v0d1
• Synonyms: Ac39; AI267038; Atp6d; P39; VATX; Vma6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC011075 sequence for NM_013477; GCCCACCCCCGCCGCAGCCATGTCGTTCTTCCCGGAGCTCTATTTCAACGTGGACAATGGCTACTTGGAG GGATTAGTGCGCGGCCTGAAGGCCGGGGTGCTCAGCCAGGCGGACTACCTCAACCTGGTGCAGTGCGAGA CGCTCGAGGACTTGAAGCTGTACCTGCAGAGTACAGATTATGGCAACTTCCTGGCCAATGAAGCGTCACC TCTGACGGTGTCAGTCATCGATGACAAGCTCAAGGAGAAGATGGTAGTAGAGTTCCGCCACATGAGAAAC CATGCTTATGAGCCGCTCGCCAGCTTCCTGGACTTCATAACTTATAGCTACATGATTGACAACGTGATCC TGCTAATCACAGGCACACTGCACCAGCGTTCAATAGCTGAACCTGTGCCCAAGTGCCATCCGCTAGGCAG CTTTGAGCAGATGGAGGCTGTGAACATCGCACAGACACCTGCAGAGCTCTACAATGCCATTCTGGTGGAC ACACCCCTGGCGGCTTTTTTCCAGGACTGCATCTCAGAGCAGGACCTTGATGAGATGAACATCGAGATAA TCCGAAATACGCTTTACAAGGCCTACTTGGAGTCCTTCTACAAGTTCTGTACTCTGTTGGGTGGGACCAC AGCTGATGCCATGTGTCCTATCCTAGAGTTTGAAGCAGACCGCCGCGCTTTCATCATCACCATCAACTCT TTCGGCACAGAGCTGTCCAAGGAGGACCGTGCCAAGCTCTTCCCGCACTGTGGCCGGCTCTACCCTGAGG GCTTGGCTCAGCTGGCGCGGGCTGATGACTATGAACAGGTCAAGAATGTAGCTGATTACTACCCGGAATA CAAACTGCTTTTTGAGGGTGCAGGTAGCAATCCTGGAGACAAGACCCTGGAGGACCGGTTTTTTGAACAT GAGGTGAAGCTAAACAAGCTGGCCTTCCTGAACCAGTTCCATTTTGGTGTCTTTTATGCCTTTGTGAAGC TTAAGGAACAGGAATGTCGCAACATCGTATGGATTGCTGAGTGCATTGCCCAGCGCCATCGCGCCAAGAT CGACAACTACATCCCCATCTTTTAGCATTCTAACTTAAGGTTCCCAAGTGCACTGTGTGTGTGTCTGTGT GCTGTGGTGAAGCCCAGGGCTCACTTGTCTGACCTGGTGAGGTGTAGCATTGTCCTGGGATTGTTTGCCC AGCTTCCATGACCCCTTCTGGGACTACTCTTAGGCCTAAAAAGGGGTTAGGCACCTCTCCCCTGATCAAG AATGGACCAAGATCCCCTCCAGAGCAGAGAGGCCCCCAGCCCTATGTTTACAGCCACTGACATGTCTAAG AAACTTGTATGACTTTCATGTCCCTCCTTAGCCTAAGGACCCCTGGGACAGTGTCCATAGGTCATCCCCA GAACCTGTGGGAAGAGTAGAGAATATGTATTCTTGGGGTATTGGGAAAGCAGCTTTGTAGCCTTCTCCCT CCGCTCTGCCCCTCTGAGACAATAAAATTGCCCTCTTTAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_013477
• Insert Size: 1056 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC011075, AAH11075
• RefSeq Size: 1525 bp
• RefSeq ORF: 1056 bp
• Locus ID: 11972
• UniProt ID: P51863
• Cytogenetics: 8 D3
• Summary: Subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. May play a role in coupling of proton transport and ATP hydrolysis. May play a role in cilium biogenesis through regulation of the transport and the localization of proteins to the cilium (By similarity). In aerobic conditions, involved in intracellular iron homeostasis, thus triggering the activity of Fe(2+) prolyl hydroxylase (PHD) enzymes, and leading to HIF1A hydroxylation and subsequent proteasomal degradation (By similarity).[UniProtKB/Swiss-Prot Function]