F3 (NM_010171) Mouse Untagged Clone

SKU MC200642

F3 (untagged) - Mouse coagulation factor III (F3), (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: F3
• Synonyms: AA409063; CD142; Cf-3; Cf3; TF
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC016397 sequence for NM_010171; GCGGGGTGGAGAGGAGCCGGTGTCCGCGCGGCCCTTTATAACGCACCCCGCGCCGACCCCGGCAGCCTGG GTACAGCCGGTACCCATCACTCGCTCCCTCCGATCGCTCCTGTAGCGTAGCCAACGCGCCGCCGCTGAAG CCCCGAGACCTCGCCTCCAGCCCTTGGACATGGCGATCCTCGTGCGCCCGCGCCTCCTAGCGGCTCTCGC GCCCACGTTTCTCGGCTGCCTCCTCCTCCAGGTGATCGCGGGTGCAGGCATTCCAGAGAAAGCGTTTAAT TTAACTTGGATATCAACTGATTTCAAGACAATTTTGGAGTGGCAACCCAAACCCACCAACTATACCTACA CTGTACAGATAAGTGATCGATCTAGAAACTGGAAAAACAAGTGCTTCTCGACCACAGACACCGAGTGCGA CCTCACAGACGAGATCGTGAAGGATGTGACCTGGGCCTATGAAGCAAAGGTCCTCTCTGTCCCACGGAGG AACTCAGTTCATGGAGACGGAGACCAACTTGTGATTCATGGGGAGGAGCCGCCATTTACAAACGCCCCAA AGTTTTTACCTTACCGAGACACAAACCTCGGACAGCCAGTAATTCAGCAGTTTGAACAAGATGGTAGAAA ACTGAACGTGGTTGTAAAAGACTCACTTACATTAGTCAGAAAGAATGGTACATTCCTCACCCTGCGGCAA GTCTTTGGCAAGGACTTGGGTTATATAATTACTTATCGGAAAGGCTCAAGCACGGGAAAGAAAACAAACA TTACAAACACCAATGAATTCTCGATTGATGTGGAAGAAGGAGTAAGCTACTGCTTTTTTGTACAAGCTAT GATTTTCTCCAGGAAAACTAACCAAAATAGCCCAGGAAGCAGTACAGTGTGCACCGAGCAATGGAAGAGT TTCCTGGGAGAAACACTCATCATTGTGGGAGCAGTGGTGCTCCTGGCCACCATCTTTATCATCCTCCTGT CCATATCTCTGTGCAAGCGCAGAAAGAACCGAGCGGGACAGAAAGGGAAGAACACCCCGTCGCGCTTGGC ATAGAGGAAAGGCTGAAGCCGCTAACGCTCACACTGCCTGCACGGCACTGTTGCGGAGAGCTCTGATGGG AACTGTGCAACATGGAGCGTGGAGCCTGCCAATCCTAGCTCAGAGAGGCTGCCTTCATGGCCTGTTACTC CAGCTAACGCTTTGATTCCAACACTAGCATTTGTCACGTTAGGACGAACTGAAACGGTACAAACTGGTTA ACACTACAGCGCCTTTTGCACAAATGCTTTAGATTGTATGGTCTACACTCAGGAAGACACTAGGTCACCC AGGCAAAGCCAGTGGACAGATGCCTTTCATATAACCTGGGTGGGCTTTTGGAAAATCTTTGAGAAGTTGA TTTCATAGGCTGTAGAACAGTAAAGTGGGAACTGGGCGGACTTTTCTAACAGTCGTACTTTTATAAAGCG GTATTTGGGTGTTTTTTCCCTCGAATAGGTACTTTTGGAAGTTCAAAGCAAGTGGCAAACTTTCATATAA ACATGTTAAATGCAGGATATTTCTGCTTGGGGCATCTTTGTGATTTGTACTTTCCTACAATTTAGCACTT TAACTGACAATGATGGGGTTTAAACATTTGACAGCCAACTCTATTTTTATACGACTACTATACAAAGAAA CTACATATAGTTTTATGATTTAAGGTACTTAGAAATGTTTATGGTTAACATTGTATATATTTACATAAAA TTTAAAGGTTTTGTATATGGGATTTTCTATTTATATAGCTTCTATTTGTATATTTTGAGATAATTTATTT AATATACTTTTATATAAATAAAGGTGATTGGGAATTGAGACAAAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_010171
• Insert Size: 885 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC016397, AAH16397
• RefSeq Size: 1880 bp
• RefSeq ORF: 885 bp
• Locus ID: 14066
• UniProt ID: P20352
• Cytogenetics: 3 52.94 cM
• Summary: This gene encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. The encoded protein binds to coagulation factor VIIa and the ensuing complex catalyzes the proteolytic activation of coagulation factors IX and X. Mice lacking encoded protein die in utero resulting from massive hemorrhaging in both extraembryonic and embryonic vessels. A severe deficiency of the encoded protein in mice results in impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis. [provided by RefSeq, Aug 2015]