Tnip1 (NM_021327) Mouse Untagged Clone

SKU MC200531

Tnip1 (untagged) - Mouse TNFAIP3 interacting protein 1 (Tnip1), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Tnip1
• Synonyms: ABIN; ABIN-1; ABIN1; AU018810; Naf1; Nef; VAN
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC008186 sequence for NM_021327; CCGGCCCCGAAGCTCTCGTCCTAGGCTAGGCGGCCGTCACGGCAGGGGTTGAGCCACCCTCATGGAAGGG AGAGGACCCTACCGGATCTACGACCCAGGGGGCAGCACGCCTCTGGGAGAGGTGTCCGCAGCTTTTGAAC GTCTAGTGGAGGAGAATACTCGGCTGAAGGGAAAAATGCAAGGGATAAAGATGTTAGGGGAGCTTCTGGA GGAGTCTCAGATGGAAGCGTCCAGACTCCGGCAGAAGGCAGAGGAGCTGGTCAAGGACAGCGAGCTGTCA CCACCGACATCTGCCCCCTCCTTGGTCTCCTTTGATGACCTGGCTGAGCTCACAGGACAGGATACAAAGG TCCAGGTACATCCTGCTACCAGCACTGCCGCCACCACCACCGCCACCGCCACCACGGGAAACTCCATGGA GAAGCCCGAGCCAGCCTCCAAATCTCCGTCCAATGGCGCCTCCTCGGACTTTGAAGTGGTCCCTACTGAG GAGCAGAATTCACCCGAAACTGGCAGCCACCCTACGAACATGATGGACCTGGGGCCCCCACCCCCAGAGG ACAGCAACCTGAAGCTCCACCTGCAGCGCCTGGAGACCACCCTTAGCGTGTGTGCAGAGGAGCCAGACCA CAGCCAGCTCTTCACCCACCTGGGCCGCATGGCCCTCGAGTTCAACAGGTTGGCCTCCAAAGTGCATAAA AATGAGCAGCGCACCTCCATCCTGCAGACCTTATGTGAGCAGCTGCGCCAGGAGAATGAAGCCCTGAAGG CCAAGCTGGACAAGGGCCTGGAACAGCGGGATCTGGCTGCTGAGAGGCTGCGGGAGGAAAACACAGAGCT CAAGAAACTGTTGATGAACAGCAGCTGCAAAGAGGGACTCTGTGGGCAGCCCAGCTCCCCAAAGCCAGAG GGTGCTGGCAAGAAGGGCGTGGCTGGACAGCAGCAGGCCAGTGTGATGGCGAGTAAAGTCCCTGAAGCGG GGGTCTTTGGAGCAGCTGAGAAGAAGGTGAAGTTGCTAGAACAGCAACGCATGGAGCTGCTGGAAGTGAA CAAGCAGTGGGACCAGCATTTCCGGTCCATGAAGCAGCAGTATGAGCAGAAGATCACAGAGCTTCGCCAG AAGCTGGTGGACCTGCAGAAACAGGTAACTGAGCTGGAGGCCGAACGGGAGCAGAAGCAGCGTGACTTTG ACCGGAAACTCCTCCTGGCCAAATCGAAGATAGAGATGGAAGAGACCGACAAGGAGCAGCTGACAGCAGA GGCCAAGGAACTGCGCCAGAAGGTCAGGTACCTACAGGATCAGCTGAGCCCGCTCACAAGGCAACGAGAA TACCAGGAGAAGGAGATCCAGCGGCTCAATAAGGCCCTGGAGGAGGCCCTCAGCATCCAGGCCTCTCCAT CATCTCCGCCTGCAGCTTTTGGGAGTCCTGAAGGCGTTGGGGGCCATCTGAGGAAGCAGGAACTGGTGAC ACAGAATGAGTTGCTGAAACAGCAGGTAAAGATCTTTGAAGAGGACTTCCAGAGGGAACGGAGTGACCGT GAACGCATGAATGAAGAGAAGGAGGAGCTGAAGAAGCAAGTAGAGAAGCTGCAGGCCCAGGTCACCCTGA CTAATGCCCAGCTCAAAACTCTCAAAGAGGAGGAGAAGGCCAAGGAAGTCCTCAAACAGCAGAAGAGGAA AGCAAAGGCTTCGGGAGAGCGCTACCACATGGAACCCCACCCTGAGCACGTCTGCGGCGCCTATCCCTAT GCCTACCCACCCATGCCAGCCATGGTACCTCACCATGCCTACAAGGACTGGTCCCAGATCCGATACCCTC CACCCCCTGTGCCCATGGAGCACCCGCCCCCACACCCCAACTCTCGCCTCTTCCATCTGCCGGAGTACAC CTGGCGTCCACCCTGTGCAGGCATTCGGAATCAGAGCTCTCAAGTGATGGACCCGCCCCCAGACAGGCCT GCAGAGCCAGAGTCTGCAGACAATGACTGTGATGGGCCCCAGTGAGGCTGCAGTGGGTCATTTGGTTCCA CCTTCATCTTTCAGAGCCAGCTGACCTCAGATTGCCAAAAGTTTGAAGGCCATGTGCATGTTCTGTGTGA CCCAAGCCTTGGCAGAGGAGAGGCTGGGATGGGTAGCTGGCTCACATCCCCAGCCAAGCCTCGAACTGTT GACAAGACCAGGGAGAACCCACCCATGGGCGCCCACCAGGTTCTTATGGATGCAAGGAGAAGCTCAACAC CCTGCCTCTTGCCAAGACAAGGAAGCCTCACCTGGCTTTGACCTGCCATCCGTTGCTGAGGCCACTGGCT TCCATCCTAAGAATGAGGTGCAACAAGACCCCATTCTCACAGAACCTCAAAGACTTGGTTCCAGGCTCTC CAGAGACCACACCCAACTCATGTGCATGTGCCGTTTTTGCTTCAAGCTCAGTAGCAGGACCTGCCCCGAG CCCCCTGCTCCTTGTCCCTCTGTGAGGAGTTACGGAGAGGGCTTTGTCTCTAGAGCAGAAGAGAATGATG GGACGGCCTGATGCTGTCATGCTCTCCACTGCACCTGTGGCAGCCTCCTGAGAGCCACCAAGATCTGGGA TGAAGGCCACACCAGCCATGTCTGCTGAAGGGCCCCAGACTGAAATGACTCCGGCCTCCACAGTTAGATG TTTATGGTGCCAGAGGTCTATATTAAGGTAGCTGTCTGTTGCTAGGCAGCCGTTTGCACAAATCTTGGAC ATAAATCCAACTTGAAGATCAAAAAAAAAAAAAAAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_021327
• Insert Size: 1944 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC008186, AAH08186
• RefSeq Size: 2767 bp
• RefSeq ORF: 1944 bp
• Locus ID: 57783
• Cytogenetics: 11 B1.3
• Summary: Inhibits NF-kappa-B activation and TNF-induced NF-kappa-B-dependent gene expression by regulating A20/TNFAIP3-mediated deubiquitination of IKBKG; proposed to link A20/TNFAIP3 to ubiquitinated IKBKG. Involved in regulation of EGF-induced ERK1/ERK2 signaling pathway; blocks MAPK3/MAPK1 nuclear translocation and MAPK1-dependent transcription. Increases cell surface CD4(T4) antigen expression. Involved in the anti-inflammatory response of macrophages and positively regulates TLR-induced activation of CEBPB. Involved in the prevention of autoimmunity; this function implicates binding to polyubiquitin. Involved in leukocyte integrin activation during inflammation; this function is mediated by association with SELPLG and dependent on phosphorylation by SRC-family kinases.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1 and 2 encode the same isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.