Gk (NM_008194) Mouse Untagged Clone

SKU MC200367

Gyk (untagged) - Mouse glycerol kinase (Gyk), transcript variant 1, (10ug)

Product Data

• Type: Mouse Untagged Clone
• Target Symbol: Gk
• Synonyms: D930012N15Rik; Gyk
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC003767 sequence for NM_008194; CTTCAGCAGAAGCGCGCTGCTTTGCCCTCCGCTGTCCCTGCTCCGCCCTCCGCAGCCCTCCACAGTCACC CCGGAGACCAGCCGTGTTAAGCTCTCTGCTCTGAAGCTGACTGACTTCCATGGCAGCCGCGAAGAAAGCA GTTCTGGGGCCATTGGTGGGAGCAGTGGACCAGGGTACCAGCTCGACACGTTTTTTGGTTTTCAATTCAA AAACAGCTGAACTTCTTAGTCATCATCAAGTAGAAATAAAACAGGAATTCCCAAGAGAAGGATGGGTAGA ACAAGACCCGAAGGAAATTCTGCAGTCTGTTTATGAGTGTATAGAGAAAACGTGTGAGAAACTTGGACAG CTCAATATTGATATTTCCAACATCAAAGCCATTGGTGTCAGCAACCAGAGGGAAACCACAGTAGTCTGGG ACAAGGTCACCGGAGAGCCTCTCTATAATGCCGTGGTGTGGCTTGACCTAAGAACCCAGTCTACTGTTGA GAACCTTAGTAAAAGAATTCCAGGAAATAATAACTTTGTCAAGTCCAAGACAGGCCTTCCACTTAGCACG TATTTCAGTGCAGTGAAACTTCGTTGGCTCCTTGACAACGTGAAAAAGGTCCAAGAGGCTGTTGAAGAAA ATAGAGCTCTTTTTGGGACCATTGATTCATGGCTTATTTGGAGTTTAACAGGAGGAATCCATGGGGGTGT CCACTGTACAGATGTAACAAATGCAAGCAGGACGATGCTTTTTAACATTCATTCTTTGGAATGGGATAAA GAGCTCTGCGAATTTTTTGGAATTCCAATGGAAATTCTTCCCAACGTTCGGAGTTCTTCTGAGATCTATG GCCTAATGAAAGCTGGGGCCTTGGAAGGTGTACCAATATCTGGGTGTTTGGGGGACCAGTCTGCTGCTTT GGTGGGACAAATGTGCTTCCAGGATGGACAGGCCAAAAACACGTATGGAACAGGGTGCTTCTTATTGTGC AACACGGGCCATAAGTGTGTATTTTCTGAACATGGCCTCCTGACAACCGTAGCATATAAACTTGGCAGAG ACAAACCTGTGTATTATGCGTTGGAAGGTTCCGTGGCTATAGCTGGTGCTGTAATCCGCTGGCTAAGAGA CAACCTTGGAATTATTAAGTCCTCTGAGGAAATTGAAAAACTTGCTAAGGAAGTAGGTACTTCTTATGGC TGCTACTTCGTTCCAGCATTTTCAGGGTTATATGCGCCTTATTGGGAGCCCAGTGCAAGAGGGATCATCT GTGGACTCACTCAGTTCACCAATAAATGTCATATCGCTTTTGCTGCACTAGAAGCTGTTTGTTTCCAAAC CCGAGAGATTTTGGATGCCATGAATCGCGACTGTGGAATTCCACTCAGTCATTTACAGGTAGATGGAGGA ATGACCAGCAATAAAATTCTTATGCAGCTACAAGCAGACATTCTGTATATTCCAGTAGTGAAACCCTCCA TGCCTGAAACAACTGCACTAGGCGCTGCCATGGCAGCTGGGGCTGCAGAGGGGGTTGGTGTGTGGAGTCT TGAACCTGAGGATTTGTCAGCTGTCACAATGGAGCGGTTTGAACCTCAGATCAATGCTGAAGAAAGCGAA ATCCGTTACTCCACATGGAAGAAAGCTGTGATGAAGTCAATTGGTTGGGTTACAACTCAGTCTCCAGAAA GTGGTATCCCATAAATAATACCACCTCACGGATTTCCAAGATGCAAGCTTTTTAATGTGATATGAAAATC TGACTATTCTGTCTCATAGTATAATGATGCTATTCATAGACTCTGATTTTTTTCATAAGCCACTGGCTGC ATGATCCTCTAAGCAGACCTATGACTTGAAATAAAGAAAGTGCAGCAGAAAGAATCCTCCAGAAACATTT AATTTTTTTTTAACATTGACAGTTAAGATCGGGTCAGTCACCTTTGAGGCTGACCTCTGCCTCCACTGTC ATGATGTCCTACACTATTCCCGTTAAGGTCTAGGGTGATTTTGTATCCTGTCTATTGAAATGTGCCATTC AGTATATTCAGATGCTAGTGGATTACACATGTTTGAGGAAGAGGTTGTTACTAACCTGTTCAAAATGAGT GGCTTCTTGCTTGTTTGCTTTTAACAGCTCAGATGTCTTCTTTTCTATATATTAGAAGGCCACAACATTA CTGGATATTTCAAATGGAAACATCTAAAGAATTGTTGGATAATGATTTGCTATTCTTGTGGCTTAGACAT TTTCTGTACAGTGTTTGCCAAAATTCAACCTTGTCAGGTGTTTTACACTGTCCACTAACTACCATAGCTT TCTGTCTGGCTCTTACAGGATAGAACACTTTCTTTTTCTGCTTTTTTTTCATTTCTCCTTTTTATATTTT TATTCTGTATGTATAACATACATGCCTATATATTTTATATGCTGAGAGTAACCCATTTATAAATTAAGAG CACATTATATTCAATAAGTTATAAGAGGGCTGGTCTTAAGTGGACTACTATGTATATAAATTGGGGGAGG GCAAGCTGTATACATTTTTGGGCAACGGTTATGCATATTATTTACCAGAAGAAAATTTTCTTATAAAATC AAACATTTGAAACTTAAGTATGTATTCAGTCAATTAAAATGTACTATATTGTAATTTCAACTATATTTCT TATACTCTACCATTTAATTATCTCAGCTGGAAGAAAAGCAACTGTGGCATGTAACAGTAAATACTCTTCT CAAACATGATTCTAGTCCAAATAAAATTTTTCCTTTGAATTGATGTTTTCCATATTTGAGCTCCTATTGA AGAATTATCAAATTCAAAAGCTAAGGGGAAAAATATGTGTGAATAAAGCCAATAATGCCTTTTAAATGTT ATTACCCATTAATAAGATGTTCTTATTTTCTGGTATGATAGAAAATTATGTCTACTTAATAACTGACAAA AAAAAAACAAGTATCATTGCAAATGCCTCACAGTATAACCAGTAACTTCTCAAATATTTAGAAGGATTGG TTATAACTCATTAACCTAGCTTCCTAACATCAATTTTAAAATGTATTTGTTTTATAGTAAAAAAAAAAAA AAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_008194
• Insert Size: 1575 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: BC003767, AAH03767
• RefSeq Size: 3083 bp
• RefSeq ORF: 1575 bp
• Locus ID: 14933
• UniProt ID: Q64516
• Cytogenetics: X 39.32 cM
• Summary: Key enzyme in the regulation of glycerol uptake and metabolism.[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded isoform (1) is shorter than isoform 4.