Becn1 (NM_019584) Mouse Untagged Clone

CAT#: MC200283

Becn1 (untagged) - Mouse beclin 1, autophagy related (Becn1), (10ug)

Specifications

Product Data

• Type: Mouse Untagged Clone
• Tag: Tag Free
• Symbol: Becn1
• Synonyms: 4921513J16Rik; 5430417M23Rik; Atg6
• Vector: PCMV6-Kan/Neo
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >BC005770 sequence for NM_019584
  GGGAAGTAGCTGAAGACCGGGCGATGGGAACTCTGGAGGTCTCGCTCCGGGGCGGACCCGAGGCCACCGT GCCCCCGCGGTAGAACGAGCCTGGGTGGCGGGCTCCGGGCTCCTGAGGCATGGAGGGGTCTAAGGCGTCC AGCAGCACCATGCAGGTGAGCTTCGTGTGCCAGCGCTGTAGCCAGCCTCTGAAACTGGACACGAGCTTCA AGATCCTGGACCGGGTCACCATCCAGGAACTCACAGCTCCATTACTTACCACAGCCCAGGCGAAACCAGG AGAGACCCAGGAGGAAGAGGCTAACTCAGGAGAGGAGCCATTTATTGAAACTCGCCAGGATGGTGTCTCT CGAAGATTCATCCCCCCAGCCAGGATGATGTCTACAGAAAGTGCTAATAGCTTCACTCTGATCGGGGAGG CATCTGATGGTGGCACCATGGAGAACCTCAGCCGGAGACTCAAGGTCACTGGAGACCTGTTTGACATCAT GTCGGGCCAGACCGATGTGGATCACCCGCTGTGTGAGGAATGCACAGACACTCTTTTAGACCAGCTGGAC ACTCAGCTCAATGTCACTGAGAATGAATGTCAGAACTACAAACGCTGTTTGGAGATCCTAGAGCAGATGA ATGAGGATGACAGTGAGCAGCTGCAGAGGGAGCTGAAGGAGCTGGCCTTGGAGGAGGAGAGGCTGATCCA GGAGCTGGAAGATGTGGAAAAGAACCGCAAGGTGGTGGCAGAGAACCTGGAGAAGGTCCAGGCTGAGGCG GAGAGATTGGACCAGGAGGAAGCTCAGTACCAGCGGGAGTATAGTGAGTTTAAAAGGCAGCAGCTGGAGT TGGATGACGAACTCAAGAGTGTGGAGAACCAGGTGCGCTACGCCCAGATCCAGCTGGACAAGCTCAAGAA AACCAATGTCTTCAATGCCACCTTCCACATCTGGCACAGCGGACAGTTTGGCACAATCAATAATTTCAGA CTGGGTCGCTTGCCCAGTGTTCCTGTGGAGTGGAATGAAATCAATGCTGCCTGGGGCCAGACAGTGCTGC TGCTCCATGCTTTGGCCAATAAGATGGGTCTGAAGTTTCAGAGGTACCGACTTGTTCCCTATGGAAATCA TTCCTATCTGGAGTCTCTGACAGACAAATCTAAGGAGTTGCCGTTATACTGTTCTGGGGGTTTGCGGTTT TTCTGGGACAACAAGTTTGACCATGCAATGGTAGCTTTTCTGGACTGTGTGCAGCAGTTCAAAGAAGAGG TGGAAAAAGGAGAGACTCGATTTTGTCTTCCGTACAGGATGGACGTGGAGAAAGGCAAGATTGAAGACAC TGGAGGCAGTGGCGGCTCCTATTCCATCAAAACCCAGTTTAACTCGGAGGAGCAGTGGACAAAAGCGCTC AAGTTCATGCTGACCAATCTCAAGTGGGGTCTTGCCTGGGTGTCCTCACAGTTCTATAACAAGTGACTTG CTCCTTAGGGGATGTTTGCCTTTAAGGTTTTATACTTTGTTTGGTTTGGAAAGATGCTTTAAATTAAATT TGGGTAATATTAAACCACATGTTTACAATACCAAAATCCACAAAAGCTACTTTATTTTCAAATATGACAG ATAGTTTCCAGAGTACGCCATGTATAGCAAAGAACCCTGCCATAGTTTTGACTCAGCCCCATGCATCCTT TCCCTCTTTCCTGAAAACAACTAATTTAAATTTGCTTTGTTTTCTTTTTTAAGTTGAATTGACGTTAATG TGTTTTCACTGGATTTTATCTCTCTCAACTTCCTGCACTTAAAATTTGAAACAGCAAAGGTTTGAGATGA GATGCTTGTGGCACACAGTTGGGTGATGTGGGGAAAGGACACCGGGTCAGGAGTTGCAAGTTTAACTCCG TCCTCACTTGTAGCATTGAATGCCTCCTGTGCTGTCTAGTGGGACTACAGAATGCTGTTTGATACTGTGT GCGACGTGGAGAGATTTAATTATTTGTAATAAAGGATTTGCTATGGTCTATTAAAAAAAAAAAAAAAAAA AAAA
• Restriction Sites: RsrII-NotI
• ACCN: NM_019584
• Insert Size: 1347 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005770, AAH05770
• RefSeq Size: 2034 bp
• RefSeq ORF: 1347 bp
• Locus ID: 56208
• UniProt ID: O88597
• Cytogenetics: 11 D
• Gene Summary: Plays a central role in autophagy (PubMed:10604474, PubMed:12372286, PubMed:19270693, PubMed:28445460). Acts as core subunit of different PI3K complex forms that mediate formation of phosphatidylinositol 3-phosphate and are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis (PubMed:19270693, PubMed:25275521). Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (By similarity). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms (PubMed:25275521). Involved in endocytosis including endosome formation in neuronal cells (PubMed:25275521). May play a role in antiviral host defense (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longest isoform (1).