Human Elastin (ELN) (NM_001081752) AAV Particle

SKU RC224648A1V

AAV ORF Particle, Serotype AAV-2, ELN (Myc-DDK-tagged)-Human elastin (ELN), transcript variant 2, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: ELN
• Synonyms: ADCL1; SVAS; WBS; WS
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC224648 ORF sequence, codon optimized.; Due to the complexity of NM_001081752, the ORF clone is codon optimized for mammalian Expression. ; The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.; ; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCAGGCCTGACAGCCGCTGCCCCAAGGCCCGGCGTGCTGTTGCTTCTGCTGTCTATTCTGCACCCTT; CTAGACCTGGGGGGGTACCGGGGGCCATTCCCGGTGGCGTCCCAGGCGGGGTGTTTTACCCCGCACTGGG; CCCAGGGGGCAAGCCTCTGAAGCCTGTGCCCGGTGGCCTCGCTGGGGCTGGCCTCGGTGCGGGCTTGGGC; GCGTTTCCCGCTGTCACCTTCCCTGGTGCCCTGGTACCAGGCGGCGTGGCCGACGCTGCGGCCGCGTACA; AAGCCGCTAAGGCGGGAGCAGGACTCGGGGGAGTACCCGGGGTAGGTGGCCTCGGCGTAAGCGCTGGAGC; CGTCGTGCCTCAGCCTGGCGCCGGAGTCAAACCTGGGAAGGTGCCCGGGGTAGGGCTGCCTGGAGTGTAC; CCAGGCGGAGTTCTCCCCGGCGCCCGCTTCCCCGGGGTGGGAGTCCTGCCAGGGGTGCCAACAGGAGCCG; GAGTGAAACCCAAAGCACCTGGCGTCGGCGGTGCGTTCGCCGGTATCCCTGGGGTAGGTCCTTTCGGGGG; CCCTCAACCCGGTGTGCCGCTTGGTTATCCCATCAAAGCCCCAAAACTTCCAGGTGGGTACGGCTTGCCA; TATACAACAGGCAAGCTCCCCTATGGCTACGGACCGGGCGGGGTGGCCGGGGCAGCCGGCAAGGCTGGAT; ACCCCACCGGCACAGGAGTGGGGCCACAGGCGGCAGCGGCCGCCGCTGCCAAGGCAGCTGCCAAATTTGG; CGCGGGCGCCGCCGGAGTCCTCCCTGGGGTTGGCGGTGCCGGCGTACCTGGGGTACCCGGCGCCATCCCC; GGGATTGGAGGAATTGCCGGTGTCGGCACACCCGCAGCAGCCGCCGCCGCCGCCGCGGCGGCCAAGGCAG; CCAAATACGGTGCCGCTGCAGGGCTGGTACCTGGAGGACCAGGTTTTGGACCCGGAGTCGTCGGAGTTCC; CGGGGCGGGAGTCCCTGGCGTCGGGGTTCCCGGCGCCGGGATCCCCGTAGTCCCCGGAGCTGGAATTCCT; GGCGCCGCCGTGCCTGGAGTTGTGAGTCCTGAAGCAGCCGCAAAAGCTGCCGCCAAAGCTGCTAAGTATG; GCGCCCGCCCCGGCGTCGGAGTGGGAGGCATTCCTACATATGGCGTGGGAGCCGGCGGGTTCCCTGGATT; CGGCGTGGGTGTCGGCGGAATTCCCGGCGTGGCCGGCGTTCCTGGCGTTGGGGGAGTTCCCGGTGTTGGC; GGGGTTCCTGGCGTGGGGATCAGCCCAGAAGCCCAAGCCGCTGCCGCTGCGAAAGCCGCCAAGTATGGAC; TGGTTCCGGGAGTCGGGGTCGCACCGGGCGTTGGAGTTGCCCCTGGCGTGGGAGTGGCACCTGGGGTGGG; GCTGGCCCCCGGTGTGGGAGTTGCTCCCGGTGTAGGAGTGGCTCCAGGTGTGGGCGTAGCACCCGGCATT; GGTCCCGGGGGCGTGGCCGCGGCCGCCAAATCTGCGGCCAAGGTTGCCGCCAAAGCACAATTGAGGGCAG; CCGCTGGTTTGGGAGCTGGTATCCCTGGGCTGGGTGTGGGAGTGGGAGTACCCGGTCTTGGTGTGGGAGC; GGGGGTTCCAGGACTCGGCGTGGGCGCCGGCGTCCCTGGATTTGGTGCAGTGCCGGGTGCCCTCGCAGCT; GCAAAAGCGGCTAAATACGGGGCGGCAGTCCCTGGAGTGCTGGGGGGCCTTGGCGCCTTGGGAGGCGTCG; GAATACCTGGAGGGGTTGTGGGCGCTGGACCAGCTGCCGCAGCCGCAGCCGCCAAGGCCGCTGCTAAGGC; TGCCCAGTTCGGCCTCGTGGGGGCCGCCGGATTGGGAGGGCTTGGCGTCGGTGGCCTCGGAGTGCCCGGG; GTGGGAGGGCTCGGTGGCATTCCTCCCGCTGCAGCAGCAAAAGCCGCCAAATACGGAGTGGCAGCCAGAC; CTGGGTTCGGCCTGAGTCCAATCTTTCCAGGAGGCGCTTGCCTCGGTAAAGCTTGCGGAAGAAAGCGCAA; G; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC224648 representing NM_001081752; Red=Cloning site Green=Tags(s); ; MAGLTAAAPRPGVLLLLLSILHPSRPGGVPGAIPGGVPGGVFYPALGPGGKPLKPVPGGLAGAGLGAGLG; AFPAVTFPGALVPGGVADAAAAYKAAKAGAGLGGVPGVGGLGVSAGAVVPQPGAGVKPGKVPGVGLPGVY; PGGVLPGARFPGVGVLPGVPTGAGVKPKAPGVGGAFAGIPGVGPFGGPQPGVPLGYPIKAPKLPGGYGLP; YTTGKLPYGYGPGGVAGAAGKAGYPTGTGVGPQAAAAAAAKAAAKFGAGAAGVLPGVGGAGVPGVPGAIP; GIGGIAGVGTPAAAAAAAAAAKAAKYGAAAGLVPGGPGFGPGVVGVPGAGVPGVGVPGAGIPVVPGAGIP; GAAVPGVVSPEAAAKAAAKAAKYGARPGVGVGGIPTYGVGAGGFPGFGVGVGGIPGVAGVPGVGGVPGVG; GVPGVGISPEAQAAAAAKAAKYGLVPGVGVAPGVGVAPGVGVAPGVGLAPGVGVAPGVGVAPGVGVAPGI; GPGGVAAAAKSAAKVAAKAQLRAAAGLGAGIPGLGVGVGVPGLGVGAGVPGLGVGAGVPGFGAVPGALAA; AKAAKYGAAVPGVLGGLGALGGVGIPGGVVGAGPAAAAAAAKAAAKAAQFGLVGAAGLGGLGVGGLGVPG; VGGLGGIPPAAAAKAAKYGVAARPGFGLSPIFPGGACLGKACGRKRK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_001081752
• ORF Size: 2031 bp
• Buffer: PBS with 0.001% Pluronic F8835
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_001081752.1, NP_001075221.1
• RefSeq Size: 3648 bp
• RefSeq ORF: 2034 bp
• Locus ID: 2006
• UniProt ID: P15502
• Cytogenetics: 7q11.23
• MW: 58.8 kDa