Human Semaphorin 3E (SEMA3E) (NM_012431) AAV Particle

SKU RC216038A1V

AAV ORF Particle, Serotype AAV-2, SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: SEMA3E
• Synonyms: coll-5; M-SEMAH; M-SemaK; SEMAH
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216038 representing NM_012431; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCATCCGCGGGGCACATTATCACCTTGCTCCTGTGGGGTTACTTACTGGAGCTTTGGACAGGAGGTC; ATACAGCTGATACTACCCACCCCCGGTTACGCCTGTCACATAAAGAGCTCTTGAATCTGAACAGAACATC; AATATTTCATAGCCCTTTTGGATTTCTTGATCTCCATACAATGCTGCTGGATGAATATCAAGAGAGGCTC; TTCGTGGGAGGCAGGGACCTTGTATATTCCCTCAGCTTGGAGAGAATCAGTGACGGCTATAAAGAGATAC; ACTGGCCGAGTACAGCTCTAAAAATGGAAGAATGCATAATGAAGGGAAAAGATGCGGGTGAATGTGCAAA; TTATGTTCGGGTTTTGCATCACTATAACAGGACACACCTTCTGACCTGTGGTACTGGAGCTTTTGATCCA; GTTTGTGCCTTCATCAGAGTTGGATATCATTTGGAGGATCCTCTGTTTCACCTGGAATCACCCAGATCTG; AGAGAGGAAGGGGCAGATGTCCTTTTGACCCCAGCTCCTCCTTCATCTCCACTTTAATTGGTAGTGAATT; GTTTGCTGGACTCTACAGTGACTACTGGAGCAGAGACGCTGCGATCTTCCGCAGCATGGGGCGACTGGCC; CATATCCGCACTGAGCATGACGATGAGCGTCTGTTGAAAGAACCAAAATTTGTAGGTTCATACATGATTC; CTGACAATGAAGACAGAGATGACAACAAAGTATATTTCTTTTTTACTGAGAAGGCACTGGAGGCAGAAAA; CAATGCTCACGCAATTTACACCAGGGTCGGGCGACTCTGTGTGAATGATGTAGGAGGGCAGAGAATACTG; GTGAATAAGTGGAGCACTTTCCTAAAAGCGAGACTCGTTTGCTCAGTACCAGGAATGAATGGAATTGACA; CATATTTTGATGAATTAGAGGACGTTTTTTTGCTACCTACCAGAGATCATAAGAATCCAGTGATATTTGG; ACTCTTTAACACTACCAGTAATATTTTTCGAGGGCATGCTATATGTGTCTATCACATGTCTAGCATTCGG; GCAGCCTTCAACGGACCATATGCACATAAGGAAGGACCTGAATACCACTGGTCAGTCTATGAAGGAAAAG; TCCCTTATCCAAGGCCTGGTTCTTGTGCCAGCAAAGTAAATGGAGGGAGATACGGAACCACCAAGGACTA; TCCTGATGATGCCATCCGATTTGCAAGAAGTCATCCACTAATGTACCAGGCCATAAAACCTGCCCATAAA; AAACCAATATTGGTAAAAACAGATGGAAAATATAACCTGAAACAAATAGCAGTAGATCGAGTGGAAGCTG; AGGATGGCCAATATGACGTCTTGTTTATTGGGACAGATAATGGAATTGTGCTGAAAGTAATCACAATTTA; CAACCAAGAAATGGAATCAATGGAAGAAGTAATTCTAGAAGAACTTCAGATATTCAAGGATCCAGTTCCT; ATTATTTCTATGGAGATTTCTTCAAAGCGGCAACAGCTGTATATTGGATCTGCTTCTGCTGTGGCTCAAG; TCAGATTCCATCACTGTGACATGTATGGAAGTGCTTGTGCTGACTGCTGCCTGGCTCGAGACCCTTACTG; TGCCTGGGATGGCATATCCTGCTCCCGGTATTACCCAACAGGCACACATGCAAAAAGGCGTTTCCGGAGA; CAAGATGTTCGACATGGAAATGCAGCTCAGCAGTGCTTTGGACAACAGTTTGTTGGGGATGCTTTGGATA; AGACTGAAGAACATCTGGCTTATGGCATAGAGAACAACAGTACTTTGCTGGAATGTACCCCACGATCTTT; ACAAGCGAAAGTTATCTGGTTTGTACAGAAAGGACGTGAGACAAGAAAAGAGGAGGTGAAGACAGATGAC; AGAGTGGTTAAGATGGACCTTGGTTTACTCTTCCTAAGGTTACACAAATCAGATGCTGGGACCTATTTTT; GCCAGACAGTAGAGCATAGCTTTGTCCATACGGTCCGTAAAATCACCTTGGAGGTAGTGGAAGAGGAGAA; AGTCGAGGATATGTTTAACAAGGACGATGAGGAGGACAGGCATCACAGGATGCCTTGTCCTGCTCAGAGT; AGCATCTCGCAGGGAGCAAAACCATGGTACAAGGAATTCTTGCAGCTGATCGGTTATAGCAACTTCCAGA; GAGTGGAAGAATACTGCGAGAAAGTATGGTGCACAGATAGAAAGAGGAAAAAGCTTAAAATGTCACCCTC; CAAGTGGAAGTATGCCAACCCTCAGGAAAAGAAGCTCCGTTCCAAACCTGAGCATTACCGCCTGCCCAGG; CACACGCTGGACTCC; ; ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA; TTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216038 representing NM_012431; Red=Cloning site Green=Tags(s); ; MASAGHIITLLLWGYLLELWTGGHTADTTHPRLRLSHKELLNLNRTSIFHSPFGFLDLHTMLLDEYQERL; FVGGRDLVYSLSLERISDGYKEIHWPSTALKMEECIMKGKDAGECANYVRVLHHYNRTHLLTCGTGAFDP; VCAFIRVGYHLEDPLFHLESPRSERGRGRCPFDPSSSFISTLIGSELFAGLYSDYWSRDAAIFRSMGRLA; HIRTEHDDERLLKEPKFVGSYMIPDNEDRDDNKVYFFFTEKALEAENNAHAIYTRVGRLCVNDVGGQRIL; VNKWSTFLKARLVCSVPGMNGIDTYFDELEDVFLLPTRDHKNPVIFGLFNTTSNIFRGHAICVYHMSSIR; AAFNGPYAHKEGPEYHWSVYEGKVPYPRPGSCASKVNGGRYGTTKDYPDDAIRFARSHPLMYQAIKPAHK; KPILVKTDGKYNLKQIAVDRVEAEDGQYDVLFIGTDNGIVLKVITIYNQEMESMEEVILEELQIFKDPVP; IISMEISSKRQQLYIGSASAVAQVRFHHCDMYGSACADCCLARDPYCAWDGISCSRYYPTGTHAKRRFRR; QDVRHGNAAQQCFGQQFVGDALDKTEEHLAYGIENNSTLLECTPRSLQAKVIWFVQKGRETRKEEVKTDD; RVVKMDLGLLFLRLHKSDAGTYFCQTVEHSFVHTVRKITLEVVEEEKVEDMFNKDDEEDRHHRMPCPAQS; SISQGAKPWYKEFLQLIGYSNFQRVEEYCEKVWCTDRKRKKLKMSPSKWKYANPQEKKLRSKPEHYRLPR; HTLDS; ; TRRLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_012431
• ORF Size: 2325 bp
• Buffer: PBS with 0.001% Pluronic F7482
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_012431.1
• RefSeq Size: 6474 bp
• RefSeq ORF: 2328 bp
• Locus ID: 9723
• UniProt ID: O15041
• Cytogenetics: 7q21.11
• MW: 89 kDa