Human RASGRP1 (NM_005739) AAV Particle

SKU RC212473A1V

AAV ORF Particle, Serotype AAV-2, RASGRP1 (Myc-DDK-tagged)-Human RAS guanyl releasing protein 1 (calcium and DAG-regulated) (RASGRP1), transcript variant 1, (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: RASGRP1
• Synonyms: CALDAG-GEFI; CALDAG-GEFII; IMD64; RASGRP
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212473 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGGCACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCTTCCCATGGCTGCAGAGCTGCCTCTAAAG; CAAGACTAGAGGCAAAGCCAGCCAACAGCCCCTTCCCCTCCCATCCCAGCTTGGCCCACATCACCCAGTT; CCGAATGATGGTGTCTCTGGGACATTTAGCCAAAGGAGCCAGCCTGGACGATCTCATTGACAGCTGCATT; CAATCTTTTGATGCAGATGGAAACCTGTGTCGAAGTAACCAACTGTTGCAAGTCATGCTGACCATGCACC; GAATTGTCATCTCCTCTGCAGAACTGCTCCAAAAAGTTATCACCCTCTATAAGGATGCTTTGGCAAAGAA; TTCACCAGGACTTTGCCTGAAGATCTGTTATTTTGTAAGGTATTGGATAACAGAATTCTGGGTCATGTTT; AAAATGGACGCCAGCTTGACAGACACTATGGAGGAGTTTCAGGAACTGGTGAAAGCTAAGGGTGAGGAGT; TACATTGCCGCCTGATTGACACAACTCAAATCAATGCCCGTGACTGGTCCAGGAAACTTACTCAAAGGAT; AAAATCAAATACCAGCAAGAAACGGAAAGTCTCCCTGCTCTTTGACCATCTGGAACCAGAAGAGCTATCC; GAGCACCTCACCTACCTTGAGTTCAAGTCTTTCCGGAGGATATCGTTCTCTGATTATCAGAATTACCTTG; TAAATAGCTGTGTGAAGGAAAACCCCACCATGGAGCGATCTATTGCTCTGTGCAACGGCATCTCCCAGTG; GGTACAACTGATGGTTCTCAGCCGCCCCACGCCGCAGCTCCGAGCAGAAGTCTTCATCAAGTTCATCCAG; GTGGCTCAGAAGCTCCACCAACTACAGAACTTCAATACACTGATGGCTGTGATAGGTGGGCTGTGTCACA; GCTCAATCTCGAGGCTCAAGGAGACAAGTTCGCATGTCCCACATGAAATCAATAAGGTTCTCGGTGAGAT; GACTGAGCTGCTGTCCTCCTCCAGAAACTACGACAATTACCGGCGAGCCTATGGAGAGTGCACCGACTTC; AAGATCCCCATTCTGGGTGTGCATCTCAAGGACCTCATCTCCCTGTATGAAGCCATGCCTGACTATCTGG; AGGACGGGAAAGTGAACGTCCATAAGCTACTGGCCCTATACAATCATATCAGTGAATTGGTCCAGCTGCA; AGAGGTGGCCCCACCCTTGGAGGCTAACAAGGACTTGGTACACTTGCTGACGTTATCCCTGGATCTTTAC; TACACTGAGGATGAAATCTATGAGCTTTCCTATGCCCGGGAACCAAGGAACCACAGAGCTCCACCACTAA; CACCTTCAAAGCCACCAGTAGTAGTGGACTGGGCTTCTGGAGTGTCTCCCAAACCTGATCCAAAAACCAT; TAGCAAACACGTCCAGAGGATGGTGGATTCTGTCTTCAAGAACTATGATCACGACCAGGATGGATACATT; TCTCAGGAAGAATTTGAAAAGATTGCTGCGAGTTTTCCATTTTCCTTCTGTGTGATGGACAAAGACAGGG; AAGGCCTCATCAGCAGGGATGAGATCACAGCCTACTTCATGAGAGCCAGCTCAATCTATTCCAAGCTGGG; CCTGGGCTTTCCTCACAACTTCCAAGAGACCACCTACCTGAAGCCCACTTTTTGTGACAACTGTGCTGGA; TTTCTCTGGGGAGTGATCAAACAAGGATATCGATGTAAAGACTGCGGGATGAACTGTCACAAACAATGCA; AAGATCTGGTTGTGTTTGAGTGTAAGAAGCGAGCCAAGAACCCAGTAGCTCCCACAGAGAACAACACTTC; TGTGGGGCCAGTGTCCAACCTTTGCTCATTGGGAGCCAAAGATCTGCTCCATGCACCTGAGGAAGGACCT; TTTACATTCCCTAATGGGGAGGCTGTGGAACATGGTGAGGAGAGTAAGGATCGGACCATCATGCTGATGG; GAGTGTCCTCACAGAAGATTTCTCTTCGGCTGAAGAGGGCTGTTGCCCACAAGGCCACCCAGACTGAATC; ACAGCCTTGGATTGGCAGTGAGGGCCCTTCAGGTCCCTTTGTGCTGTCTTCCCCAAGGAAGACAGCCCAG; GATACTCTATATGTGCTTCCCAGTCCCACCTCTCCATGTCCTAGCCCAGTCTTGGTCAGAAAGCGGGCTT; TTGTCAAGTGGGAGAATAAAGACTCCCTCATAAAATCAAAGGAGGAGCTCCGTCACCTCAGACTGCCTAC; CTACCAAGAACTGGAACAGGAAATAAATACTCTGAAAGCAGATAATGATGCCCTAAAGATCCAACTGAAA; TATGCACAGAAGAAAATAGAATCCCTCCAGCTTGAAAAAAGCAATCATGTCTTAGCTCAAATGGAGCAGG; GTGACTGTTCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212473 protein sequence; Red=Cloning site Green=Tags(s); ; MGTLGKAREAPRKPSHGCRAASKARLEAKPANSPFPSHPSLAHITQFRMMVSLGHLAKGASLDDLIDSCI; QSFDADGNLCRSNQLLQVMLTMHRIVISSAELLQKVITLYKDALAKNSPGLCLKICYFVRYWITEFWVMF; KMDASLTDTMEEFQELVKAKGEELHCRLIDTTQINARDWSRKLTQRIKSNTSKKRKVSLLFDHLEPEELS; EHLTYLEFKSFRRISFSDYQNYLVNSCVKENPTMERSIALCNGISQWVQLMVLSRPTPQLRAEVFIKFIQ; VAQKLHQLQNFNTLMAVIGGLCHSSISRLKETSSHVPHEINKVLGEMTELLSSSRNYDNYRRAYGECTDF; KIPILGVHLKDLISLYEAMPDYLEDGKVNVHKLLALYNHISELVQLQEVAPPLEANKDLVHLLTLSLDLY; YTEDEIYELSYAREPRNHRAPPLTPSKPPVVVDWASGVSPKPDPKTISKHVQRMVDSVFKNYDHDQDGYI; SQEEFEKIAASFPFSFCVMDKDREGLISRDEITAYFMRASSIYSKLGLGFPHNFQETTYLKPTFCDNCAG; FLWGVIKQGYRCKDCGMNCHKQCKDLVVFECKKRAKNPVAPTENNTSVGPVSNLCSLGAKDLLHAPEEGP; FTFPNGEAVEHGEESKDRTIMLMGVSSQKISLRLKRAVAHKATQTESQPWIGSEGPSGPFVLSSPRKTAQ; DTLYVLPSPTSPCPSPVLVRKRAFVKWENKDSLIKSKEELRHLRLPTYQELEQEINTLKADNDALKIQLK; YAQKKIESLQLEKSNHVLAQMEQGDCS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005739
• ORF Size: 2391 bp
• Buffer: PBS with 0.001% Pluronic F6891
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005739.2
• RefSeq Size: 5048 bp
• RefSeq ORF: 2394 bp
• Locus ID: 10125
• UniProt ID: O95267
• Cytogenetics: 15q14
• MW: 90.4 kDa