Human GSDME (NM_004403) AAV Particle

SKU RC210515A1V

AAV ORF Particles, serotype AAV-2, DFNA5 (Myc-DDK-tagged)-Human deafness, autosomal dominant 5 (DFNA5), transcript variant 1, 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: GSDME
• Synonyms: DFNA5; ICERE-1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC210515 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTTGCCAAAGCAACCAGGAATTTTCTTAGAGAAGTTGATGCTGATGGTGACCTGATTGCAGTATCAA; ATCTGAATGACTCTGATAAGTTACAGCTTCTAAGTCTGGTGACAAAAAAGAAGAGATTCTGGTGCTGGCA; GAGACCCAAGTACCAGTTTTTATCCCTCACCCTTGGCGATGTACTCATAGAAGACCAATTTCCGAGTCCA; GTGGTCGTGGAGTCGGACTTTGTGAAATACGAGGGCAAGTTTGCAAACCACGTGAGTGGAACCCTGGAGA; CTGCACTGGGGAAGGTCAAGCTGAACCTGGGGGGCAGCAGCCGCGTAGAGAGCCAGTCTTCATTTGGAAC; CCTGAGGAAGCAGGAGGTGGATTTGCAGCAGCTCATCAGAGACTCTGCCGAGAGAACAATAAATCTGAGA; AACCCTGTGCTCCAGCAGGTGCTGGAGGGAAGGAATGAGGTCCTGTGCGTTTTGACACAGAAGATCACGA; CGATGCAGAAGTGTGTGATCTCTGAGCACATGCAGGTCGAGGAGAAGTGTGGTGGCATCGTGGGCATCCA; GACCAAGACGGTGCAGGTGTCAGCGACGGAGGATGGGAATGTCACCAAGGACTCCAACGTGGTGCTGGAG; ATCCCAGCTGCCACCACCATTGCCTACGGTGTCATTGAGTTATACGTGAAACTGGACGGCCTGTTCGAGT; TCTGCCTTCTCCGAGGGAAGCAAGGTGGCTTCGAGAACAAGAAGAGAATTGACTCTGTCTACCTGGACCA; CCTGGTCTTTCGAGAGTTTGCATTCATAGACATGCCAGATGCTGCGCATGGGATATCTTCCCAGGATGGA; CCATTAAGTGTTTTAAAGCAAGCGACCCTGCTCCTGGAGAGGAATTTCCATCCATTTGCGGAGCTGCCTG; AGCCACAACAGACAGCTTTGAGTGACATCTTCCAGGCGGTCGTATTTGATGATGAACTACTCATGGTCCT; GGAACCAGTGTGCGATGACCTGGTCAGCGGCCTCTCGCCCACAGTGGCGGTGCTGGGGGAGCTGAAGCCC; CGGCAGCAGCAGGACCTTGTGGCCTTCCTGCAGCTGGTGGGGTGCAGCTTACAGGGTGGGTGTCCGGGCC; CCGAGGATGCAGGCAGCAAGCAGCTGTTTATGACAGCCTACTTCTTGGTCAGTGCCCTCGCAGAAATGCC; AGATAGCGCAGCAGCTCTGCTGGGCACTTGCTGCAAACTCCAGATCATTCCCACACTGTGCCACTTGCTT; CGTGCTCTGTCTGATGATGGAGTATCTGATCTTGAAGACCCAACCTTGACTCCCCTGAAAGATACAGAAA; GGTTTGGGATTGTGCAGCGCTTGTTTGCCTCAGCTGACATTAGTCTGGAGAGACTGAAGTCATCTGTGAA; AGCTGTCATTCTGAAGGACTCTAAAGTCTTCCCACTGCTTCTTTGTATAACCCTGAATGGACTCTGTGCT; TTAGGCAGAGAACATTCA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC210515 protein sequence; Red=Cloning site Green=Tags(s); ; MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSP; VVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLR; NPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLE; IPAATTIAYGVIELYVKLDGLFEFCLLRGKQGGFENKKRIDSVYLDHLVFREFAFIDMPDAAHGISSQDG; PLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVVFDDELLMVLEPVCDDLVSGLSPTVAVLGELKP; RQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLL; RALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCA; LGREHS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_004403
• ORF Size: 1488 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_004403.2
• RefSeq Size: 2521 bp
• RefSeq ORF: 1491 bp
• Locus ID: 1687
• UniProt ID: O60443
• Cytogenetics: 7p15.3
• MW: 54.6 kDa