Human TLE 1 (TLE1) (NM_005077) AAV Particle

SKU RC204037A1V

AAV ORF Particle, Serotype AAV-2, TLE1 (Myc-DDK-tagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1), (10ug), 250ul, 10^13 TU/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: TLE1
• Synonyms: ESG; ESG1; GRG1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC204037 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCTGCAGGCCAGCCCTTCAAGTTCACTATCCCGG; AGTCCCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATG; TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGA; TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATCC; CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAGGTGACCATGGC; AGAGTTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTTTCTCATGGCCACGGACCCCCA; GTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCTGGAATCCCGCCCCTCGGGGGCAGTGCCGGCC; TTCTTGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGA; TGCAGAGCACCACAGAGACAGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGC; ACAGATAAACGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGACT; CCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCTAATGAGGACCC; TTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATCGACAAAAATCGCCTGCTAAAG; AAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCGGCAAGTTCCACTTCTTTGAAATCCAAAGAAA; TGAGCTTGCATGAAAAAGCCAGCACGCCTGTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCC; AACGCCGGGCACCAGCGCCACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTC; GTTAACCAAGCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGGA; TGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGTTTACACAACAT; GTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTACGGGCGCTCCCCCATGGTGGGG; TTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCTCCAAACCTGGCAGGAATCCCTGGGGGGAAAC; CTGCATACTCCTTCCACGTTACTGCAGACGGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCAT; CGGACCCGGAATCCCCCGGCATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTG; ACCATCAGCAACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGCC; ACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATCCGTTCCTGTAA; ATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACTTTGTCCATTTGGGACCTGGCG; GCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCGGCCCCCGCCTGCTACGCCCTGGCCATCAGCC; CCGATTCCAAGGTCTGCTTCTCATGCTGCAGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGAC; ACTAGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAG; CTCTGGACGGGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAGC; ACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCAGTGGGCATGGA; GAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAGCTGCACCTGCATGAGAGCTGC; GTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTTGTGAGTACTGGAAAAGATAACCTCCTCAATG; CTTGGCGGACCCCCTATGGAGCCAGCATATTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACAT; CTCTGTGGATGATAAGTACATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC204037 protein sequence; Red=Cloning site Green=Tags(s); ; MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG; LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQQLQAQHLSHGHGPP; VPLTPHPSGLQPPGIPPLGGSAGLLALSSALSGQSHLAIKDDKKHHDAEHHRDREPGTSNSLLVPDSLRG; TDKRRNGPEFSNDIKKRKVDDKDSSHYDSDGDKSDDNLVVDVSNEDPSSPRASPAHSPRENGIDKNRLLK; KDASSSPASTASSASSTSLKSKEMSLHEKASTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAIDPL; VNQAAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYASLHNMSPQMSAAAAAAAVVAYGRSPMVG; FDPPPHMRVPTIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAV; TISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLA; APTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTK; LWTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESC; VLSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005077
• ORF Size: 2310 bp
• Buffer: PBS with 0.001% Pluronic F5517
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005077.3, NP_005068.2
• RefSeq Size: 3283 bp
• RefSeq ORF: 2313 bp
• Locus ID: 7088
• UniProt ID: Q04724
• Cytogenetics: 9q21.32
• MW: 83.2 kDa