Human nSMase (SMPD2) (NM_003080) AAV Particle

SKU RC200417A1V

AAV ORF Particles, serotype AAV-2, SMPD2 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 2, neutral membrane (neutral sphingomyelinase) (SMPD2), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: nSMase
• Synonyms: ISC1; NSMASE; NSMASE1
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200417 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAAGCCCAACTTCTCCCTGCGACTGCGGATCTTCAACCTCAACTGCTGGGGCATTCCGTACTTGAGCA; AGCACCGGGCCGACCGCATGAGGCGCCTGGGAGACTTTCTGAACCAGGAGAGCTTCGACCTGGCTTTGCT; GGAGGAGGTGTGGAGTGAGCAGGACTTCCAGTACCTGAGACAGAAGCTGTCACCTACCTACCCAGCTGCA; CACCACTTCCGGAGCGGAATCATTGGCAGTGGCCTCTGTGTCTTCTCCAAACATCCAATCCAGGAGCTTA; CCCAGCACATCTACACTCTCAATGGCTACCCCTACATGATCCATCATGGTGACTGGTTCAGTGGGAAGGC; TGTGGGGCTGCTGGTGCTCCATCTAAGTGGCATGGTGCTCAACGCCTATGTGACCCATCTCCATGCCGAA; TACAATCGACAGAAGGACATCTACCTAGCACATCGTGTGGCCCAAGCTTGGGAATTGGCCCAGTTCATCC; ACCACACATCCAAGAAGGCAGACGTGGTTCTGTTGTGTGGAGACCTCAACATGCACCCAGAAGACCTGGG; CTGCTGCCTGCTGAAGGAGTGGACAGGGCTTCATGATGCCTATCTTGAAACTCGGGACTTCAAGGGCTCT; GAGGAAGGCAACACAATGGTACCCAAGAACTGCTACGTCAGCCAGCAGGAGCTGAAGCCATTTCCCTTTG; GTGTCCGCATTGACTACGTGCTTTACAAGGCAGTTTCTGGGTTTTACATCTCCTGTAAGAGTTTTGAAAC; CACTACAGGCTTTGACCCTCACAGTGGCACCCCCCTCTCTGATCATGAAGCCCTGATGGCTACTCTGTTT; GTGAGGCACAGCCCCCCACAGCAGAACCCCAGCTCTACCCACGGACCAGCAGAGAGGTCGCCGTTGATGT; GTGTGCTAAAGGAGGCCTGGACGGAGCTGGGTCTGGGCATGGCTCAGGCTCGCTGGTGGGCCACCTTCGC; TAGCTATGTGATTGGCCTGGGGCTGCTTCTCCTGGCACTGCTGTGTGTCCTGGCGGCTGGAGGAGGGGCC; GGGGAAGCTGCCATACTGCTCTGGACCCCCAGTGTAGGGCTGGTGCTGTGGGCAGGTGCATTCTACCTCT; TCCACGTACAGGAGGTCAATGGCTTATATAGGGCCCAGGCTGAGCTCCAGCATGTGCTAGGAAGGGCAAG; GGAGGCCCAGGATCTGGGCCCAGAGCCTCAGCCAGCCCTACTCCTGGGGCAGCAGGAGGGGGACAGAACT; AAAGAACAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200417 protein sequence; Red=Cloning site Green=Tags(s); ; MKPNFSLRLRIFNLNCWGIPYLSKHRADRMRRLGDFLNQESFDLALLEEVWSEQDFQYLRQKLSPTYPAA; HHFRSGIIGSGLCVFSKHPIQELTQHIYTLNGYPYMIHHGDWFSGKAVGLLVLHLSGMVLNAYVTHLHAE; YNRQKDIYLAHRVAQAWELAQFIHHTSKKADVVLLCGDLNMHPEDLGCCLLKEWTGLHDAYLETRDFKGS; EEGNTMVPKNCYVSQQELKPFPFGVRIDYVLYKAVSGFYISCKSFETTTGFDPHSGTPLSDHEALMATLF; VRHSPPQQNPSSTHGPAERSPLMCVLKEAWTELGLGMAQARWWATFASYVIGLGLLLLALLCVLAAGGGA; GEAAILLWTPSVGLVLWAGAFYLFHVQEVNGLYRAQAELQHVLGRAREAQDLGPEPQPALLLGQQEGDRT; KEQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_003080
• ORF Size: 1269 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_003080.2
• RefSeq Size: 1697 bp
• RefSeq ORF: 1272 bp
• Locus ID: 6610
• UniProt ID: O60906
• Cytogenetics: 6q21
• MW: 47.6 kDa