HIF1AN (NM_017902) Human 3' UTR Clone

SKU SC222457

3' UTR clone of hypoxia inducible factor 1 alpha subunit inhibitor (HIF1AN) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: HIF1AN
• Synonyms: FIH1
• ACCN: NM_017902
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222457 3’UTR clone of NM_017902 ; The sequence shown below is from the reference sequence of NM_017902. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AACACAATGATCAAGGGCCGATACAACTAGCCTGCCAGGGGTCAAGGCCTCCTGCCAGGTGACTGCTAT; CCCGTCCACACCGCTTCATTGATGAGGACAGGAGACTCCAAGCGCTAGTATTGCACGCTGCACTTAATG; GACTGGACTCTTGCCATGGCCCAGGAGTCAGGTGTTTGGAGCGAGGCAGGGCAGTTGGCACTCCACTCC; TATTTGGAGGGACTTCATACCCTTGCCTCTTGTGCCCCAGCACCTTCTCTCTCTGCCCCCCGCCTAAAG; TCCTGCATTCAGTGTGTGGAGTCCCAGCTTTTGGTTGTCATCATGTCTGTGTGTATGTTAGTCTGTCAA; CTTCGGAATGTGTGCGTGTGTGTGCATGCACACGCATGTATGTATCTGTTCCCTGTTCCTTCTGGGTCA; GGCTGTCACTTCCGGCTCTCAGCCCTATCTCCTGCAACCTCAGTGCCTCAGCCTGAGAGAGAGATGAGA; TGCTCTTGGACTCCCCACTGCATCTGGGCTGCAGGGCCAGAGCTAGTCTGACCATTAGGTCAGTCTGCC; TCCTGACAGTTTTTGCGTAGTCAAGCTCTAGGCGGTATGGGAATGGCTACCGGGACTCTAATGGGGTGA; AAGAGAGGGGAGGCTTGCCTTTGAGAGCCTATATAGCCTTCCTGTGAGAGAGGATTAGATAGGGTTCCA; ACTGGGCCTACAAGCTCAAGCCATACATAAAAGGACCTTGGGACATAAGAACCAATGATTGTGCATAAG; TTCTAAATTAGAGACACATATAGTTTCTCTCTTTCAGCACCAGCTCTTGCCCCTATGCTGGGTACCAAG; GGAGTTCTCCTAGCTGTGGCTTCTCTAGGTTCTAGGGGTGCAAGCCTCTGTGTGTTTGTTTGTGTGTGT; CTGTGTGTGCGTATCCACACTAGGGGTGCAAGCCTCTGGGTGTGTGTGTGTGTGTGCGTGCGTGTGTGT; GTGTGTGTCCGTGTGTGTGTGTGTGTGTGTCCACACTGGCCAGCCTCCCTACTTACCAAGGTTCTCCAC; TGCTTACCTTTTCCAGTGGGACAGTACAGTGTGAGCCCCCGGGAAGTACTGCCTGACCTATCCTAAGCT; TTTACACTTGGATTTTAGCCATCATATGTTGGCCAGGTCTCACTGCAGCCTGCCCGAGGCTAACTGGCT; AGAGCCTCCAGGCCCTATGATGCTCCCTGCCCAGGCCATATCCTTTATTCCTGCTGAGCTTCCTGGCTG; AATAGATGAAATGGGGTCAAGCCCAGGCAGCTCATTCACTACCTGTGATCCACCTCAGGGCACGGGCAA; ACACATAGGCTTGCGTCTTAAAGCCAGCTCCTCTGCCAGACCCCGTTGTAATGTGCCACAACACCCTCA; ATAGTCAGGGCAACTGGTGGAGCATGGAAGTCGAATTTCCTTTTCTGTTAGGAGCTACTCCTGGGAACC; CCTCTCAGGGCTGCAGCTTACAGGTGGGCAGCTGTGATTGCACAACTTGAAGGGCCATCATTCACATCT; ATTCAGTGGGAGTGGGGTCCCTGGGATTGGGCAGTGTGGTGGCCCTGTGTCTCCTCACCTCTGCTCCTG; TCTTCATCACCTTCTCTCTGGAAGGGAAGAGGAGTTGGAAGGTCTCTGGTTTTCTTTTCTTTTTTTTTT; TTTTTGCCAAAGGTTTACTTCCAGCATCTGAGCTCTGGCTCTCACCCCTGAAGCTCAGTTATAGTGCAC; TGATGAACTGAGAGGATGCGTGTGGATGTGTGTGCATGCCTGAGTGCGTTTTTTGGGGAGGGGTGTTTA; TTTTTAGTACCCCATTCTGGGGTTCTCTGATGCAGTGTGGATGTGAAGATATGGTACCTTCTCAAGTGT; AGCTCTTTCAAATATAGTCAATGCTGGGAAATGTGATTGCAGTGATCTCTATCTCTCCACTTCTTTTGG; GAAAGAGGAGCACAGGAGCGGAGGAAAAACTTGGCCACAGTCACACTTGGAAAGATAGTAGATTATTT; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017902.3
• Locus ID: 55662
• Summary: Hydroxylates HIF-1 alpha at 'Asn-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300-interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.[UniProtKB/Swiss-Prot Function]