PLAG1 (NM_001114634) Human 3' UTR Clone

SKU SC222303

3' UTR clone of pleiomorphic adenoma gene 1 (PLAG1) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PLAG1
• Synonyms: PSA; SGPA; SRS4; ZNF912
• ACCN: NM_001114634
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222303 3’UTR clone of NM_001114634 ; The sequence shown below is from the reference sequence of NM_001114634. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTCCCACGTTTCCATCAAGCTTTTCAGTAGGATTCTGGGACATGGATTCATTACAGAAATGTATGTGTA; GCTGTGCCCTAGATGACCATTTTTATTTTAGTGCCTACTTTAAAACAGTATAAAAATTTCTGCTTTTGT; ATAATACAAATTTTCATTAAGCCAGTATAAAATAGAAACTAGCTTTTAAACTGAGCTTTGGAACCATTT; GTGTTCAGTTAAGTTTACCTGGGTATTTTGTCCTGATTCACTGCCAATTGTCACATTTTAAGACTTTTT; TTTTTCCATATAGGAAAGCCATTATTAGTAGTAAACTTTTACAAATCCCATTTTCAAATTACTTTTAGA; TCTTAAAATTTTCATTTTTGTCTAATAACAGTGGCTCTACCTTTTGACATCTGGCTCATTAAAAAATTT; AGCAATAGAATGTAAATTGTATAAAAAGTTTGTGAATAACTCAAGGGTTTAAATTTTCTTACTAGCTTC; TAAATGGATTAATAATCAAGTGCTTCAAATGAATTAAGAGTCCAGTTTCGGAAGATAATAAATGTTTGT; TAGATACACCATAATTTCAGATCAGTATATTCTGAAGACTCCCTGTTGTCTGGCTAAAATATTTGCCAT; CTTTATTATGAGCCTTTAAGGAAAACAAACCCTAAACACAAAGCATCAGTATTTATAGCAAAAAGAGAC; TCTGTTAGGTGACATGGCATTTCGTGTCACTTAATAGTTGGCCCTAAATTAGTACACAGGATATTTTGT; CGTGTTTCATCCTTCTTAACATGCTATCTTTTCATTTAATAATAGTAATAGTGTATGGCATTGGGGTCT; TCAGAGTCGATATATAGGTAGATCTCTTTAGTCTTTTCCACCTTTCACATCCAAGGGGTGGGTCAAGTG; CAGCCAGCAATTTATTTTCATTGTTGGCCCACGGTTAGTCCATAATCTAGAGCCATTGTGGAACTGCAG; CCATGAGGTGTGTTTATCCCACAGTGGATTGACTCAGCCTCTGTGGGTGACAGACTTCTAAGCAGGAAG; ATAGACGTGAAGCACATGGTTACATTTGGGAACTTGTGTAGGGATCATGGCCCCTGTAGCCAGGGTTAA; AAACTGGACTTTTTAGAAGTAAAGTAAAAGCATATCGCTTATATCATTTCTTGCTGAATTTGATATGTT; TTTCTTTCCCTTAAGAATCAAAAGCAGAAAACAAAAACAACAGTCCTACTCCGATGTTATCTTTCTGAT; TCAATGTGAATCCATCTTTCCTTGCAATATTTTGGATGGAGAATTTGAAGTTAAATGCATTAGAAAACT; ACCTGATGAACTACCACAAAGTTTTAAGTGACTAGAAATATATACAGTAAAATCCCACTTTCATGCATC; TCTGGGAAATGATAGGAGTATTGCAAATAAGTTGAGTTTGTAGAGGGTAACAAAGTAAAGTAAAACAAA; CCTATCTTGGTTAACATGAAAATAACAATTGAGAATATATTATATTCACTGAATAATTATAGGCTTTTC; CTCACATTAGACAACCAACATAATCTTCTTAAAGGTCTAATTAATATATTTTTCTAAGGGTCAGTTGGG; ACATTAACCTAAGAAACATATCTATTAAGCACTTGTTAACACCTTATTTTAGGACCCTTTCCGTTGGGG; ATGGGGGCAAGGGTGGGAGGTTTTTAGAAGAGTATATATCTCTTTAAAAAAAAACAGAAAGAAAAATAT; TTCTGAGCACTCATTAGCCCTATATGGAAACTTCTTTCCTTTTTGTAGGGCCAGTTATCACTGCAGATT; GCAATGTTTACCAAGAATTTCTAAAAATGAGTGCAGATTACTGAATATAATACATTATTTAAAATATTT; GGGAGTAGTATAATTTGTGGAGAAATGTAAATTGTAATAATGTAAATGGGGGCTTCAATATATATATAT; AATACACACACACACACACATGCACACATACCGCACTTCATAGAATCAAAGTTGCTCTCTGAAGGAGC; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001114634.2
• Locus ID: 5324
• Summary: Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]