RGS5 (NM_003617) Human 3' UTR Clone

SKU SC222178

3' UTR clone of regulator of G-protein signaling 5 (RGS5) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: RGS5
• Synonyms: MST092; MST106; MST129; MSTP032; MSTP092; MSTP106; MSTP129
• ACCN: NM_003617
• Insert Size: 5074 bp
• Sequence Data:
  Insert Sequence

  >SC222178 3' UTR clone of NM_003617; The sequence shown below is from the reference sequence of NM_003617. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon ; ; ; CAATTGGCAGAGCTCAGAATTCAAGCGATCGC; ; TCGCTTTGTGCGCTCTGAGTTTTATCAGGAGTTAATCAAGTAGTAATTTAGCCAGGCTATGAAATCATCC; TGTGAGTTATTTCCTCCATAATAACCCTGCATTTCCCATTAATCTACATATCTTCCCACAGCAGCTTTGC; TCAGTGATACCCACATGGGAAAAATCCCAGGGGATGTTGCTTACTCTTTTTGCCCACACTGCTTTGGATA; CTTATCTACTGTCCGAAGGCCTTCTTTCCCCACTCAATTCTTCCTGCCCTGTTATTAATTAAGATATCTT; CAGCTTGTAGTCAGACACAATCAGAATCACAGAAAAATCCTGCCTAAGGCAAAGAAATATAAGACAAGAC; TATGATATCAATGAATGTGGGTTAAGTAATAGATTTCCAGCTAAATTGGTCTAAAAAAGAATATTAAGTG; TGGACAGACCTATTTCAAAGGAGCTTAATTGATCTCACTTGTTTTAGTTCTGATCCAGGGAGATCACCCC; TCTAATTATTTCTGAACTTGGTTAATAAAAGTTTATAAGATTTTTATGAAGCAGCCACTGTATGATATTT; TAAGCAAATATGTTATTTAAAATATTGATCCTTCCCTTGGACCACCTTCATGTTAGTTGGGTATTATAAA; TAAGAGATACAACCATGAATATATTATGTTTATACAAAATCAATCTGAACACAATTCATAAAGATTTCTC; TTTTATACCTTCCTCACTGGCCCCCTCCACCTGCCCATAGTCACCAAATTCTGTTTTAAATCAATGACCT; AAGATCAACAATGAAGTATTTTATAAATGTATTTATGCTGCTAGACTGTGGGTCAAATGTTTCCATTTTC; AAATTATTTAGAATTCTTATGAGTTTAAAATTTGTAAATTTCTAAATCCAATCATGTAAAATGAAACTGT; TGCTCCATTGGAGTAGTCTCCCACCTAAATATCAAGATGGCTATATGCTAAAAAGAGAAAATATGGTCAA; GTCTAAAATGGCTAATTGTCCTATGATGCTATTATCATAGACTAATGACATTTATCTTCAAAACACCAAA; TTGTCTTTAGAAAAATTAATGTGATTACAGGTAGAGGCCTTCTAGGTGAGACACTTTTAAGGTACACTGC; ATTTTGCAAAAAAAAAAAAAAAAAGTAATCTTTTAGCAACCCCAGTATTCCTTCACTATTTCGCTTCCTG; CATTAGCAAATTTTACTTACAGTCAAAAGTGCAGATTTATACTCCTGACGTGTCTCATTCACAGCTAAAT; AATAGGCCATAGGACTTTTGGTAGGTTTAAACTTTTAATTCTGTATTTCATGATTATAAGTCTTGCTAGA; ATTTTTTCTAATCTTTAGTAGATTTGATTAAATAATGATTCACAGAATTTAGTAACAGAATCAAACTAAG; CCATGTATGAGGGTAATCGAGATGAGGATATTAACTCAAAAGAAATAGGGTGATTTTTAAAGGATTAATA; AAATTCTGAAATGTTAAGTAGAAGATTACATTGTCTAGTCTTGTATTTCCTCCTTCTGTTGCTCTCTTTC; ATTCACACACTCTCAGTTTCTCATATTTGTAGCTCATTTATTTGGTTATTTCCTAAGAATATTGAAAGTG; AAGCAACTATGTGACTGTATTCTTCAGGTAAACACTGACTGCGCTTGTTGGATTTTCCCTATTTTTGTGA; CTTCAAGAATAATATGCCCTGCTGAATACATGCCATTTCACATTCTGAAACTGGGTAGAGTGGTTGGGTG; TTCTGCCAACAATTGCTAGTGGTGTGAATTCATTCATATTTGCCAGTATTGCTCACTTCAAAGAAACTCC; TTCATCAAGCAGTCCAGAGCTAGGCCAGATCAATGCTACAATCATGAAGTTCTCATTGCATGCAATTGTG; TAGGATTGACAAGGAACTCAGATAAAAATTTCCAGGGTGCACTTCCAGAACCAGCTTCAACATATGTCTA; CATTGCCCCCAAGTTAATAAAGTGCCAACCCTTTACTCTCTCATACAGCCAGAAATGTTAGAAATCCAAA; ATCTTGGTGCATTATTTTTTCATAAACGCTAAAACATTTGAAGAAACAATTTAATTATTTAAAATTCAAG; TATTTTATTCACATTATTTGCAATATCCAAATGTTTAAAAATTCCCAGATAATTAACTAGCTATTACAGA; TCTCACCTAGAGGGTTGATGTTATGAAGACTCCAGTGGACTGTACTCACAAATTGACTGGACACCCTATG; AAAGTGGGTAGACCTCTCAGCGGAAAATAAGAAGGGCTTTTACCTACAGGGCAGGACAGGGTCCCATGAG; AGCAGTTCTGTGGAGATATAAAAAGAATGGAAGAAGGAATGCCTTATAGTGATATTGTGACATTATATCT; ATATATCTACATATATCTATCTATCTATATCTACATCTATATAATCTTACATTTAAAATTGTATTCCTAC; ACATATTAGAAACTCTTCTAATAAATGAAGTAAAAAAATTAAAAAGAATACAAATATTCCAGCCCCAAAT; GAGAAATCAAACATATTAAAATTGTTCAAGAAAATTTCTTTGAACACTTCTGAAAGTTTTTGGAAACTTA; GAAAAGAGGGAAAAAAATCCAGTGTTACTAGTAATTTCCATGGTAATACAGATAAAATACATTCTTTTAA; TTCTGGGAAATTAGAAAAAGTGGGGTGATCTTTCCAGGAAAAACATGTGTAACATCTGCTTATCACTCCA; GCTCCCTCCTCCTCCTCCTCTCCACGTTCCCTTGAGTAAATGTCTGGGAAAGCATGAAGCTTGATGCAAG; AACCCTGTTGTACTGGCGTTTTCCTCCCCTGTGAAAACGTAACTACTGTTGGGAGTGAATTGAGGATGTA; GAAAGGTGGTGGAACCAAATTGTGGTCAATGGAAATAGGAGAATATGGTTCTCACTCTTGAGAAAAAAAC; CTAAGATTAGCCCAGGTAGTTGCCTGTAACTTCAGTTTTTCTGCCTGGGTTTGATATAGTTTAGGGTTGG; GGTTAGATTAAGATCTAAATTACATCAGGACAAAGAGACAGACTATTAACTCCACAGTTAATTAAGGACG; TATGTTCCATGTTTATTTGTTAAAGCAGTGTGAATAGCCTTCAAGCATGTGAATAATCTTCCATCTTCCC; CGCCACACATACACACACACACTTTTTGTTTCTTTCAGGTAGACACCTTTTAAAATGCAGAACTAACTGA; GGCATTTCAGTAACTTTGCTTTCAAATCAATAAAGTCAAATGTATGGAAACATTTTGTGCCCTACTCTCC; ATACCCCGTGTACTCAAATTCTCTACTGTATGAATTATGCTTTAAGTAGAATTCAGTGCCAAGGAGAACT; TGGTGAAATAAATTATTTTAATTTTTTTTTTATCCTTTACAAAGCCATGGATTTTATTTGGTTGATGTGT; GCTCTGTACACAAGCCATTTCAATAGGATGGAGCTGTTAATTATTTTCCAAAGAGTAATAGACATGCAAA; AGTTTCAATAAAAACTGGGCCATTAACAAATAAATTAATAAACTAATAAGCATTCCCTTCTAGGTTTTTG; CCAAACTGCCTATCCAATAACAAATTTGAGAATCGTTGAAAAAGCTAGTTATATTTCAGAGAAATGATTT; TCATTATTGAAACTGTTCTCCCTAGCAGGCCATTTTCCCTTTTTCCTGGGAGTTTAGCAAGTTTAGGAGA; GAATAGTCATGAAAAGAAAGGGAAGAAAGGGGAGAAGGGAAGAGGTTAAAAAGTAAGTGCTCAGACCTAT; GAACGTAATCCCTTTGCTAGAAATATTTAAGAGCAGCTCAGCTTGGTTGAAACTGAGTTTTGTCATCTTC; CATATTTGCAGGAAGGTATTTTCTGACTTGCAATGCAGCTAGATGTAAAATTTTATTTTATCATCCTAGA; AAGCCTTGACTAGAAAAATGAATAAATATTGAGGGTTTCCTGTCCATATCTGGCTTGCATGTGCCAGAAA; GCAGAGAATAGAAAATGTAATCTCCAACATCCAAGCATCGAAACCCAAGGGGTAGGCAATTCTATGTAGG; TTTTGGACATGAAGTTTGGTGCATCTTGGTTTATGCTGGCTCAACTGCTATTAAACCTCTCTGGCTTATA; GTCTCTTCATTCTATTAGACAAGCACGTATCGAACACTTGCTTCGCACAAGGCTCTTTAGTTAACAATTT; AGCAGCTACTGTTTGTGTTAAACACACTTTTCACCAAATAGGTTCTGAGGCAAACGAGAGCAATGACTAT; TTAAAGAAAGGCTTTCCCAGCATCACTTACACATCCCAAAACTAAAAAGATCAACTCTTCCAACTGAGAA; AAGACTCCTGGCTTTGAATGGAAACTTACAGCAGAGAGTCACAGGCCACGGCAACAACAACGACAACAAC; AAACATTTGGAATATTATTCTCAACTCACGTTTTAATAATACATCTTATTATTTTTCTAGTAGAGAAACT; ACAAATCAGCCTCTTCAACATTTATATACAGTTTAATAAGCCTCTTGCAAGTTACTTGTTCTCTCACCTG; AGGTATTTTTTTCCTCCCCACCTTGCCCCTGTTCCTCCCTTCCTCTTCTCCCTTTGCAAGAGGAAATATT; TAACATATTTGGGTCCAACTTCAATAATGTAATAATTAATACATTAAAAGCATTTAACTTCCTTTCTAGA; AAAATGCACAGGCTAAGGCATAGACAAAACAAAGAGAAATGCTGAGAAATTTGCCACTGGAGACAAGCAA; TCTGAATAAATATTTGCCAAAAGTTCTTTTTATGTCATATAGTGTCAGGATTTGAAGGAGCTATTTTTTT; TTAATGTTGCAACTAGCAACTCATCTTCGGAAGACACAGCCAGGAGAATGAAGTAGAAGTGAAAGGTTTA; TAAATCCATTTGTAAGCATTTATCCCATATATTTTAAATTCAAGAAAAATTGTGTTTATCTTTAGAATTT; TGTATTCAATACTTTATGTACTATGTGACTCATGCTTCTGGATAAATAAAGCACCAAATATGTATCTGTA; ACCACAATCACACATATTATATTAAATATATATC; ; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_003617.2
• Locus ID: 8490
• Summary: This gene encodes a member of the regulators of G protein signaling (RGS) family. The RGS proteins are signal transduction molecules which are involved in the regulation of heterotrimeric G proteins by acting as GTPase activators. This gene is a hypoxia-inducible factor-1 dependent, hypoxia-induced gene which is involved in the induction of endothelial apoptosis. This gene is also one of three genes on chromosome 1q contributing to elevated blood pressure. Alternatively spliced transcript variants have been identified. [provided by RefSeq, Dec 2011]