Hamartin (TSC1) (NM_000368) Human 3' UTR Clone

SKU SC222100

3' UTR clone of tuberous sclerosis 1 (TSC1) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Hamartin
• Synonyms: LAM; TSC
• ACCN: NM_000368
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC222100 3’UTR clone of NM_000368 ; The sequence shown below is from the reference sequence of NM_000368. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TACAATGAGACTCATCATGAACACAGCTAAGGAATGATGGTCAATCAGTGTTAACTTGCATATTGTTGG; CACAGAACAGGAGGTGTGAATGCACGTTTCAAAGCTTTCCTGTTTCCAGGGTCTGAGTGCAAGTTCATG; TGTGGAAATGGGACGGAGGTCCTTTGGACAGCTGACTGAATGCAGAACGGTTTTTGGATCTGGCATTGA; AATGCCTCTTGACCTTCCCCTCCACCCGCCCTAACCCCCTCTCATTTACCTCGCAGTGTGTTCTAATCC; AAGGGCCAGTTGGTGTTCCTCAGTAGCTTTACTTTCTTCCTTTCCCCCCCAAATGGTTGCGTCCTTTGA; ACCTGTGCAATATGAGGCCAAATTTAATCTTTGAGTCTAACACACCACTTTCTGCTTTCCCGAAGTTCA; GATAACTGGGTTGGCTCTCAATTAGACCAGGTAGTTTGTTGCATTGCAGGTAAGTCTGGTTTTGTCCCT; TCCAGGAGGACATAGCCTGCAAAGCTGGTTGTCTTTACATGAAAGCGTTTACATGAGACTTTCCGACTG; CTTTTTTGATTCTGAAGTTCAGCATCTAAAGCAGCAGGTCTAGAAGAACAACGGTTTATTCATACTTGC; ATTCTTTTGGCAGTTCTGATAAGCTTCCTAGAAAGTTCTGTGTAAACAGAAGCCTGTTTCAGAAATCTG; GAGCTGGCACTGTGGAGACCACACACCCTTTGGGAAAGCTCTTGTCTCTTCTTCCCCCACTACCTCTTA; TTTATTTGGTGTTTGCTTGAATGCTGGTACTATTGTGACCACAGGCTGGTGTGTAGGTGGTAAAACCTG; TTCTCCATAGGAGGGAAGGAGCAGTCACTGGGAGAGGTTACCCGAGAAGCACTTGAGCATGAGGAACTG; CACCTTTAGGCCATCTCAGCTTGCTGGGCCTTTTGTTAAACCCTTCTGTCTACTGGCCTCCCTTTGTGT; GCATACGCCTCTTGTTCATGTCAGCTTATATGTGACACTGCAGCAGAAAGGCTCTGAAGGTCCAAAGAG; TTTCTGCAAAGTGTATGTGACCATCATTTCCCAGGCCATTAGGGTTGCCTCACTGTAGCAGGTTCTAGG; CTACCAGAAGAGGGGCAGCTTTTTCATACCAATTCCAACTTTCAGGGGCTGACTCTCCAGGGAGCTGAT; GTCATCACACTCTCCATGTTAGTAATGGCAGAGCAGTCTAAACAGAGTCCGGGAGAATGCTGGCAAAGG; CTGGCTGTGTATACCCACTAGGCTGCCCCACGTGCTCCCGAGAGATGACACTAGTCAGAAAATTGGCAG; TGGCAGAGAATCCAAACTCAACAAGTGCTCCTGAAAGAAACGCTAGAAGCCTAAGAACTGTGGTCTGGT; GTTCCAGCTGAGGCAGGGGGATTTGGTAGGAAGGAGCCAGTGAACTTGGCTTTCCTGTTTCTATCTTTC; ATTAAAAAGAATAGAAGGATTCAGTCATAAAGAGGTAAAAAACTGTCACGGTACGAAATCTTAGTGCCC; ACGGAGGCCTCGAGCAGAGAGAATGAAAGTCTTTTTTTTTTTTTTTTTTTTTTAGCATGGCAATAAATA; TTCTAGCATCCCTAACTAAAGGGGACTAGACAGTTAGAGACTCTGTCACCCTAGCTATACCAGCAGAAA; ACCTGTTCAGGCAGGCTTTCTGGGTGTGACTGATTCCCAGCCTGTGGCAGGGCGTGGTCCCAACTACTC; AGCCTAGCACAGGCTGGCAGTTGGTACTGAATTGTCAGATGTGGAGTATTAGTGACACCACACATTTAA; TTCAGCTTTGTCCAAAGGAAAGCTTAAAACCCAATACAGTCTAGTTTCCTGGTTCCGTTTTAGAAAAGG; AAAACGTGAACAAACTTAGAAAGGGAAGGAAATCCCATCAGTGAATCCTGAAACTGGTTTTAAGTGCTT; TCCTTCTCCTCATGCCCAAGAGATCTGTGCCATAGAACAAGATACCAGGCACTTAAAGCCTTTTCCTG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000368.5
• Locus ID: 7248
• Summary: This gene is a tumor suppressor gene that encodes the growth inhibitory protein hamartin. The encoded protein interacts with and stabilizes the GTPase activating protein tuberin. This hamartin-tuberin complex negatively regulates mammalian target of rapamycin complex 1 (mTORC1) signalling which is a major regulator of anabolic cell growth. This protein also functions as a co-chaperone for Hsp90 that inhibits its ATPase activity. This protein functions as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients, including Tsc2 and thereby preventing their ubiquitination and proteasomal degradation. Mutations in this gene have been associated with tuberous sclerosis. [provided by RefSeq, Apr 2018]