RICTOR (NM_152756) Human 3' UTR Clone

SKU SC221814

3' UTR clone of RPTOR independent companion of MTOR complex 2 (RICTOR) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: RICTOR
• Synonyms: AVO3; hAVO3; PIA
• ACCN: NM_152756
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221814 3’UTR clone of NM_152756 ; The sequence shown below is from the reference sequence of NM_152756. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CCTATAGTTGATACATCTGCTGAATCCTGACCTCATATTTATGATGGATATAGATACATACTATATATA; TTCATATTTGTGGATTTCCTAAAAGCCTCAGAAAATACGACTGACTAGGCAGCAAAGACAGGAGTATCT; TCTGTACACTGTTCCGCAGTTACTGGTACATGAACAGTTGGAACTGCTGACTTTCCTAACCAAAACAAC; TTCCTTCTCTCCTTTGTTGAGCCTTTTGAGGGGTTCATGATTCATTACCACAGTTTTAAGAGTTTCAGT; TACCATTGTATGCAAGAGCCAAGCACTGAATACCTACATAGGTTTTCTATTTTCTTTCATTTTAAAAGC; ATAATGACAGTGGAACAATAATGGGATATGCAGAAGCACCCTTCACAAGTTATTTCTGAATGATTTTTA; GGGTAAATAATACAGATGCCTTGTTTGTTAACTAACTTGTGGAAAGCAGGAATCAGTGTCTCTAAGGCT; GCATCCTATTACCACAATGGGGTGTGCTATAACTGCTGGTATTAGAGAGGGAACTTTGGCCCTTTCACG; TTTTTCTTAATGTTTGTAACACTACTTCAGAGGTTTATAACCTCAAAGCAGAAGAAGAGCCTCAACAAC; CCGGGACTTATAAGTTATTTTTATGTTACTAGACTTGCATAAAGATTCTTGTTTTCCAACTCTTCATTT; TGTTGCAATGTGTTATTACAGGATATATGAACCAATTAAGGTTTTTCACTACAGTTCTTGAATAAAATT; TAAAAATCATTTTTTATTTTAATTAAAAATATTTCCCATTTATAGAATGCATATATTTGCAATGGACTT; CCACTTTCATCAACTTTCCATCTCATCGCTTTAAACAGGAACTTGAACAAGCACTGTTAGTTTAGACCT; AAAGGATAGGAAAGCATTAAATAATACTTTGGATCTCCTGAGGAAAAGATAAGTTTGCTTGCAATTTAC; ACATTCCATGGGGAAAGAAGAGCCATATTTCCTTAAAAAAAACATTAATAAAGCTTGTTATTGAGAAAA; ATTGTAGTGAAAAGCCTTAAGTACCAAATTTTAAAGCAGCAGTAACTTAATTTTTATATCAGTGTTTTT; GTTTTGCACAAACTAAATGCAGTGGTAGGTGGGTTTATGAGTATATTAATTGCCTTTATCCATTTGTGA; AGTTAAGTTGATGAGGGCAAGGTTTTTGTTTGTTTAATTTGTATATGTCTAAAGGTATTTGGAACTTTT; TACAGGAATTAAACATATATGCAAATTTGTATATAAAAATAGCATGGCCATCATTTGAATGCTTGTAAA; TGAAAGGATTATCTTTTTTGAGATCTATATATAAATAGAAATAGAAAATCCAGCTGGACTGATTAGGAT; TCTTTTTTAATTCATTTGTGTATAACATTTTTATTACAATTACACATCAGTTTTGACACAGTCATAGCA; ACATTAATATTTTCCCATGATGCAGATCCTTTTTGTAATGGGCTTGTTCTTTGAGATCTCTGTAAAGAA; CCCTGTGAACTAGAAAACATAACTCACAGAGATACTTTTTTAAAAAATTTATTTACTGGAACTGAAAGT; TCCAGTTGGGATGAAGCATTTCATCTCACTTCATAACACCTCTTTGACTGCACTTCAGTGAATTGTTCT; TATGTGCACTGTGTAGCAACTTACATTATAACAAAGCAGATAAGGGCTGTAAGCTGCTGCTTATGTTGA; AAAGTGGTTCTTCAGATTTTCTCTCATAAAATCCAGTTGAAGATAAATAATTTTTTTATACTTTATCAC; TGAACCCAAGTGTTTATTTAAATGTCAACAGTACTTCTAAGAACGTTGCCTGTCATCGTGGTCTTTGGT; CTTGGATAACTAAACTGCCTTTCCAGAGAACCAAATGTCAGAGTTACTAGACCAAATAGTGGTTAAAAC; CTCCAAAGGAAGTAATGTAATCTTATTCATAATGGGATTAACATATTTTAGACATTCATTTTAAACAC; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152756.5
• Locus ID: 253260
• Summary: RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 [PubMed 15268862]).[supplied by OMIM, Mar 2008]