PODXL (NM_001018111) Human 3' UTR Clone

SKU SC221530

3' UTR clone of podocalyxin-like (PODXL) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PODXL
• Synonyms: gp135; Gp200; PC; PCLP; PCLP-1; PDX; PODXL1
• ACCN: NM_001018111
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221530 3’UTR clone of NM_001018111 ; The sequence shown below is from the reference sequence of NM_001018111. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTGGATGAGGAGGAAGACACACACCTCTAGTCCGGTCTGCCGGTGGCCTCCAGCAGCACCACAGAGCTC; CAGACCAACCACCCCAAGTGCCGTTTGGATGGGGAAGGGAAAGACTGGGGAGGGAGAGTGAACTCCGAG; GGGTGTCCCCTCCCAATCCCCCCAGGGCCTTAATTTTTCCCTTTTCAACCTGAACAAATCACATTCTGT; CCAGATTCCTCTTGTAAAATAACCCACTAGTGCCTGAGCTCAGTGCTGCTGGATGATGAGGGAGATCAA; GAAAAAGCCACGTAAGGGACTTTATAGATGAACTAGTGGAATCCCTTCATTCTGCAGTGAGATTGCCGA; GACCTGAAGAGGGTAAGTGACTTGCCCAAGGTCAGAGCCACTTGGTGACAGAGCCAGGATGAGAACAAA; GATTCCATTTGCACCATGCCACACTGCTGTGTTCACATGTGCCTTCCGTCCAGAGCAGTCCCGGGCAGG; GGTGAAACTCCAGCAGGTGGCTGGGCTGGAAAGGAGGGCAGGGCTACATCCTGGCTCGGTGGGATCTGA; CGACCTGAAAGTCCAGCTCCCAAGTTTTCCTTCTCCTACCCCAGCCTCGTGTACCCATCTTCCCACCCT; CTATGTTCTTACCCCTCCCTACACTCAGTGTTTGTTCCCACTTACTCTGTCCTGGGGCCTCTGGGATTA; GCACAGGTTATTCATAACCTTGAACCCCTTGTTCTGGATTCGGATTTTCTCACATTTGCTTCGTGAGAT; GGGGGCTTAACCCACACAGGTCTCCGTGCGTGAACCAGGTCTGCTTAGGGGACCTGCGTGCAGGTGAGG; AGAGAAGGGGACACTCGAGTCCAGGCTGGTATCTCAGGGCAGCTGATGAGGGGTCAGCAGGAACACTGG; CCCATTGCCCCTGGCACTCCTTGCAGAGGCCACCCACGATCTTCTTTGGGCTTCCATTTCCACCAGGGA; CTAAAATCTGCTGTAGCTAGTGAGAGCAGCGTGTTCCTTTTGTTGTTCACTGCTCAGCTGATGGGAGTG; ATTCCCTGAGACCCAGTATGAAAGAGCAGTGGCTGCAGGAGAGGCCTTCCCGGGGCCCCCCATCAGCGA; TGTGTCTTCAGAGACAATCCATTAAAGCAGCCAGGAAGGACAGGCTTTCCCCTGTATATCATAGGAAAC; TCAGGGACATTTCAAGTTGCTGAGAGTTTTGTTATAGTTGTTTTCTAACCCAGCCCTCCACTGCCAAAG; GCCAAAAGCTCAGACAGTTGGCAGACGTCCAGTTAGCTCATCTCACTCACTCTGATTCTCCTGTGCCAC; AGGAAAAGAGGGCCTGGAAAGCGCAGTGCATGCTGGGTGCATGAAGGGCAGCCTGGGGGACAGACTGTT; GTGGGAACGTCCCACTGTCCTGGCCTGGAGCTAGGCCTTGCTGTTCCTCTTCTCTGTGAGCCTAGTGGG; GCTGCTGCGGTTCTCTTGCAGTTTCTGGTGGCATCTCAGGGGAACACAAAGCTATGTCTATTCCCCAAT; ATAGGACTTTTATGGGCTCGGCAGTTAGCTGCCATGTAGAAGGCTCCTAAGCAGTGGGCATGGTGAGGT; TTCATCTGATTGAGAAGGGGGAATCCTGTGTGGAATGTTGAACTTTCGCCATGGTCTCCATCGTTCTGG; GCGTAAATTCCCTGGGATCAAGTAGGAAAATGGGCAGAACTGCTTAGGGGAATGAAATTGCCATTTTTC; GGGTGAAACGCCACACCTCCAGGGTCTTAAGAGTCAGGCTCCGGCTGTAGTAGCTCTGATGAAATAGGC; TATCCACTCGGGATGGCTTACTTTTTAAAAGGGTAGGGGGAGGGGCTGGGGAAGATCTGTCCTGCACCA; TCTGCCTAATTCCTTCCTCACAGTCTGTAGCCATCTGATATCCTAGGGGAAAAGGAAGGCCAGGGGTTC; ACATAGGGCCCCAGCGAGTTTCCCAGGAGTTAGAGGGATGCGAGGCTAACAAGTTCCAAAAACATCTG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001018111.3
• Locus ID: 5420
• Summary: This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin. [provided by RefSeq, Jul 2008]