TIMP3 (NM_000362) Human 3' UTR Clone

SKU SC221118

3' UTR clone of TIMP metallopeptidase inhibitor 3 (TIMP3) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: TIMP3
• Synonyms: HSMRK222; K222; K222TA2; SFD
• ACCN: NM_000362
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221118 3’UTR clone of NM_000362 ; The sequence shown below is from the reference sequence of NM_000362. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AAAAGCATCATCAATGCCACAGACCCCTGAGCGCCAGACCCTGCCCCACCTCACTTCCCTCCCTTCCCG; CTGAGCTTCCCTTGGACACTAACTCTTCCCAGATGATGACAATGAAATTAGTGCCTGTTTTCTTGCAAA; TTTAGCACTTGGAACATTTAAAGAAAGGTCTATGCTGTCATATGGGGTTTATTGGGAACTATCCTCCTG; GCCCCACCCTGCCCCTTCTTTTTGGTTTTGACATCATTCATTTCCACCTGGGAATTTCTGGTGCCATGC; CAGAAAGAATGAGGAACCTGTATTCCTCTTCTTCGTGATAATATAATCTCTATTTTTTTAGGAAAACAA; AAATGAAAAACTACTCCATTTGAGGATTGTAATTCCCACCCCTCTTGCTTCTTCCCCACCTCACCATCT; CCCAGACCCTCTTCCCTTTGCCCTTCTCCTCCAATACATAAAGGACACAGACAAGGAACTTGCTGAAAG; GCCAACCATTTCAGGATCAGTCAAAGGCAGCAAGCAGATAGACTCAAGGTGTGTGAAAGATGTTATACA; CCAGGAGCTGCCACTGCATGTCCCAACCAGACTGTGTCTGTCTGTGTCTGCATGTAAGAGTGAGGGAGG; GAAGGAAGGAACTACAAGAGAGTCGGAGATGATGCAGCACACACACAATTCCCCAGCCCAGTGATGCTT; GTGTTGACCAGATGTTCCTGAGTCTGGAGCAAGCACCCAGGCCAGAATAACAGAGCTTTCTTAGTTGGT; GAAGACTTAAACATCTGCCTGAGGTCAGGAGGCAATTTGCCTGCCTTGTACAAAAGCTCAGGTGAAAGA; CTGAGATGAATGTCTTTCCTCTCCCTGCCTCCCACCAGACTTCCTCCTGGAAAACGCTTTGGTAGATTT; GGCCAGGAGCTTTCTTTTATGTAAATTGGATAAATACACACACCATACACTATCCACAGATATAGCCAA; GTAGATTTGGGTAGAGGATACTATTTCCAGAATAGTGTTTAGCTCACCTAGGGGGATATGTTTGTATAC; ACATTTGCATATACCCACATGGGGACATAAGCTAATTTTTTTACAGGACACAGAATTCTGTTCAATGCT; GTTAAATATGCCAATAGTTTAATCTCTTCTATTTTGTTGTCGTTGCTTGTTTGAAGAAAATCATGACAT; TCCAAGTTGACATTTTTTTTTCATTTTAATTAAAATTTGAAATTCTGAACACCGTCAGCACCCTCTCTT; CCCTATCATGGGTCATCTGACCCCTGTCCGTCTCCTTGTCCCTGCTTCATGTTTGGGGGCCTTTCTTTA; ACTGCCTTCCTGGCTTAGCTCAGATGGCAGATGAGAGTGTAGTCAAGGGCCTGGGCACAGGAGGGAGAG; CTGCAGAGTGTCCTGCCTGCCTTGGCTGGAGGGACACCTCTCCTGGGTGTGGAGACAGCTTGGTTCCCT; TTCCCTAGCTCCCTGGTGGGTGAATGCCACCTCCTGAGATCCTCACCTCTTGGAATTAAAATTGTTGGT; CACTGGGGAAAGCCTGAGTTTGCAACCAGTTGTAGGGTTTCTGTTGTGTTTTTTTTTTTTTTTTTGAAA; TAAAACTATAATATAAATTCTCCTATTAAATAAAATTATTTTAAGTTTTAGTGTCAAAAGTGAGATGCT; GAGAGTAGGTGATAATGTATATTTTACAGAGTGGGGGTTGGCAGGATGGTGACATTGAACATGATTGCT; CTCTGTCTCTTTTTTCAGCTTATGGGTATTTATCTTCTATTAGTATTTGTATCTTCAGTTCATTCCACT; TTAGGAAACAGAGCTGCCAATTGAAACAGAAGAAGAAAAAAAAAAAAAGCAGCAGACAACACACTGTAG; AGTCTTGCACACACACAAGTGCCCAGGCAAGGTGCTTGGCAGAACCGCAGAGTGGGAAGAGAGTACCGG; CATCGGGTTTCCTTGGGATCAATTTCATTACCGTGTACCTTTCCCATTGTGGTCATGCCATTTGGCAG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_000362.5
• Locus ID: 7078
• Summary: This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]