ATM (NM_138292) Human 3' UTR Clone

SKU SC221016

3' UTR clone of ataxia telangiectasia mutated (ATM) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: ATM
• Synonyms: AT1; ATA; ATC; ATD; ATDC; ATE; DKFZp781A0353; MGC74674; TEL1; TELO1
• ACCN: NM_138292
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC221016 3’UTR clone of NM_138292 ; The sequence shown below is from the reference sequence of NM_138292. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; CTTTTCCCAGGATGGAAAGCTTGGGTGTGATCTTCAGTATATGAATTACCCTTTCATTCAGCCTTTAGA; AATTATATTTTAGCCTTTATTTTTAACCTGCCAACATACTTTAAGTAGGGATTAATATTTAAGTGAACT; ATTGTGGGTTTTTTTGAATGTTGGTTTTAATACTTGATTTAATCACCACTCAAAAATGTTTTGATGGTC; TTAAGGAACATCTCTGCTTTCACTCTTTAGAAATAATGGTCATTCGGGCTGGGCGCAGCGGCTCACGCC; TGTAATCCCAGCACTTTGGGAGGCCGAGGTGAGCGGATCACAAGGTCAGGAGTTCGAGACCAGCCTGGC; CAAGAGACCAGCCTGGCCAGTATGGTGAAACCCTGTCTCTACTAAAAATACAAAAATTAGCCGAGCATG; GTGGCGGGCACCTGTAATCCCAGCTACTCGAGAGGCTGAGGCAGGAGAATCTCTTGAACCTGGGAGGTG; AAGGTTGCTGTGGGCCAAAATCATGCCATTGCACTCCAGCCTGGGTGACAAGAGCGAAACTCCATCTCA; AAAAAAAAAAAAAAAAAACAGAAACGTATTTGGATTTTTCCTAGTAAGATCACTCAGTGTTACTAAATA; ATGAAGTTGTTATGGAGAACAAATTTCAAAGACACAGTTAGTGTAGTTACTATTTTTTTAAGTGTGTAT; TAAAACTTCTCATTCTATTCTCTTTATCTTTTAAGCCCTTCTGTACTGTCCATGTATGTTATCTTTCTG; TGATAACTTCATAGATTGCCTTCTAGTTCATGAATTCTCTTGTCAGATGTATATAATCTCTTTTACCCT; ATCCATTGGGCTTCTTCTTTCAGAAATTGTTTTTCATTTCTAATTATGCATCATTTTTCAGATCTCTGT; TTCTTGATGTCATTTTTAATGTTTTTTTAATGTTTTTTATGTCACTAATTATTTTAAATGTCTGTACTT; GATAGACACTGTAATAGTTCTATTAAATTTAGTTCCTGCTGTTTATATCTGTTGATTTTTGTATTTGAT; AGGCTGTTCATCCAGTTTTGTCTTTTTGAAAAGTGAGTTTATTTTCAGCAAGGCTTTATCTATGGGAAT; CTTGAGTGTCTGTTTATGTCATATTCCCAGGGCTGTTGCTGCACACAAGCCCATTCTTATTTTAATTTC; TTGGCTTTAGGGTTTCCATACCTGAAGTGTAGCATAAATACTGATAGGAGATTTCCCAGGCCAAGGCAA; ACACACTTCCTCCTCATCTCCTTGTGCTAGTGGGCAGAATATTTGATTGATGCCTTTTTCACTGAGAGT; ATAAGCTTCCATGTGTCCCACCTTTATGGCAGGGGTGGAAGGAGGTACATTTAATTCCCACTGCCTGCC; TTTGGCAAGCCCTGGGTTCTTTGCTCCCCATATAGATGTCTAAGCTAAAAGCCGTGGGTTAATGAGACT; GGCAAATTGTTCCAGGACAGCTACAGCATCAGCTCACATATTCACCTCTCTGGTTTTTCATTCCCCTCA; TTTTTTTCTGAGACAGAGTCTTGCTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTG; AAACCTCTGCCTCCTGGGTTCAAGCAATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGACTACAGGCGT; GTGCCAACACGCCCGGCTAATTTTTTGTATTTTTATTAGAGACGGAGTTTCACCGTGTTAGCCAGGATG; GTCTCGATCGCTTGACCTCGTGATCCACCCTCCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGC; CACCGCGCCCGGCCTCATTCCCCTCATTTTTGACCGTAAGGATTTCCCCTTTCTTGTAAGTTCTGCTAT; GTATTTAAAAGAATGTTTTCTACATTTTATCCAGCATTTCTCTGTGTTCTGTTGGAAGGGAAGGGCTTA; GGTATCTAGTTTGATACATAGGTAGAAGTGGAACATTTCTCTGTCCCCCAGCTGTCATCATATAAGAT; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_138292.3
• Locus ID: 472
• Summary: The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]