AKT2 (NM_001626) Human 3' UTR Clone

SKU SC220977

3' UTR clone of v-akt murine thymoma viral oncogene homolog 2 (AKT2) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: AKT2
• Synonyms: HIHGHH; PKBB; PKBBETA; PRKBB; RAC-BETA
• ACCN: NM_001626
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC220977 3’UTR clone of NM_001626 ; The sequence shown below is from the reference sequence of NM_001626. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TTCTCCTACTCGGCCAGCATCCGCGAGTGAGCAGTCTGCCCACGCAGAGGACGCACGCTCGCTGCCATC; ACCGCTGGGTGGTTTTTTCCCCCTAACTTTTTACTTAGCCTTTTTGGTTTGTGTCCCCACCCCCACCTC; CTCACCCCCTTTCCAGTTCTTCTTCAGGCCCCTCCCAGACGCACCCCAGCGGCCCCTGCAGCCCCTGCC; TCCAGCCTCCAGCCTCACCTTTGTGCCCAGACTCGCATTTGGAAGACTCCACCTCCCGCCCAGGCCTGG; GCTGTTGGGCGGTTGGAGATTCAGGTTTTAATCCACACAAGCCCCAGTGAGGGGTGAAGCATGGCGCCT; GGGGCCTGCCTGAGTTTCTGGCCTGGGTGTCGTGCTGGTGTCTGCCTCCGCGCTGCTGCATCTGGACGA; AGGCTGCCTTCTGGTGGGACGCGACACCCGGCAGACAGTGGTGCTGCCTTCCAGGCCCCGTGGCCTAGG; CTCGGAGTGGCCAGGCACGGGGCGGTCCAATCCCCCACCCGCTGTCCCCCTATGGGGGCAGAAAAGCAA; TAATGTCCAGGGGCAGGCAGGGGCCCTTGGGAGCTGCAGGGCTGGGGGTTAGGGCTGCTCCCTGGTGAA; TGGAGTCAGATCCTAGGATCTGTACCATGGGGAACCAGGAGTGGCCGGGCTGGGTGCCGCCTCCTGGTC; CGGCCTCCTCCCCACCAAACTGTCCTCACCCTATGGATGAGGCAGGAGGAACATTTGGGGCCAAACCTG; CCTGCCTCCCAGCCCCGTGCCTTACTAGGGCTTCCTTCCAGCTGGCCTTACCTCCCGCTGGACCCTGGG; CCTGGCCTGGCCCCACTGGGGGCTATGGGCTGGGCTCACCCTCTCCTCTGCGGGGGTGGAGGGCCACCA; GCCTTGGCTGTTACAATCTTACACCGGACAGTATTGGGCCCCATGGACTTGGTCAGGGAGGGGTGGGGG; TGGGCATCTCTGGTACCTATTGGGGTGGGGGGCCTCTGAAAAGGGAGGCTCCTAGGCCCCCCTCACCCC; TCCCTCTCCCCAGGGCCCCACGTTCTGCAGCCTTAAGGTTGAACATGAGTGCACGTCCATGTCAGTGCT; GTGGGACTCCTGTGCGTGCCTCGGACTGCGTGTGTCGGCGGGACGCAGGCACACGTGGGTGTGTGTGCA; TGTGTGTTTGTGTGAGGGCAGCGTGTCCTCCAGTGTGCATGGTGTGTGGGCTTGGGCCCCATCCCTGGC; CCGAGCATTTCATCCTGTGGGGGAGGGGTGCTGACCTAGTGGGAGGAGCCCCACTGTGATCCATGAGCT; GCCCTGCCCACGCCTCCCCTCCCTGTAGCAACACCTCTGGGTGTTTGGAGTTTAGCTTTTGTGGGTTTG; CTCTCCCTATCCCATCTCCTGTACTACACAGTTCATGGCAGGGTGGGGAGGGGTGGGGTTGGTTCGGGT; GGGTGAGGGTCTTTTTCCTCTGTGTGCGATGTTGTTATCTGACAGTTCTCCGTCCCTACTGGCCTTTCT; CCTCGTCTTCATATTTGTACGGTACAAGCAATAAAGACACTCATTTCAGACCAGGGCCCAGCCTGCACT; CACGCCAGCCCAACCACTCTGGGCTTTGCCTTGGTGATGGAGTCAGACCCCTGGGCCCCAGCTCCTCCT; GTACTAGCCGTTCCCTTCAGCAAGGAGGGCACTGAGCTCAGGGTGAGGGCAGCTGGGGTGTGTGCAGGA; GCTCAGGCTGGAGAGGGTGGGTGGAGCTGGTGCTGTGGGGCTGAGGGGTATGGGAAGGCTCCCCGCATG; TGGGGGTGGGGTGGACAGAGACCACTCCAGGCCCTCAGTGCTGCTTAGGCTAAGAGAGGTGGGGTGGAG; GGACAGGGCTGGAAGATCTGGGTAGCCCAGAATGAGGAGGGTGCCTGTGCTGTCACTGAATGAGAGGGA; GTGGTTCATTCCACCCGGCTGCCGAGCCTCAGAGGGGGGCATTCCTATCCTGCCCCACCTCCCTGTTT; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001626.6
• Locus ID: 208
• Summary: This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. The gene serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins, and has also been implicated in insulin signaling. [provided by RefSeq, Nov 2019]