DYRK1A (NM_101395) Human 3' UTR Clone

SKU SC220938

3' UTR clone of dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1A (DYRK1A) transcript variant 3 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: DYRK1A
• Synonyms: DYRK; DYRK1; HP86; MNB; MNBH; MRD7
• ACCN: NM_101395
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC220938 3’UTR clone of NM_101395 ; The sequence shown below is from the reference sequence of NM_101395. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AGCACTGGATGCCAGGTGCCTTTAGAATGACCGTATCATTTACCCTAGAGGTTCATGACGTTCCTGTCT; AAGGAAATGTGCGTCAGCAATTTCCTGCTCCTCTTGGTTGGTCAGGCACTGAAGCTCCTACACAGGTCA; CTGTTGAAACTCATCCTGTTCAAGAAACAACCTTTCATGTAGCCCCTCAACAGAATGCATTGCATCATC; ACCATGGTAACAGTTCCCATCACCATCACCACCACCACCACCATCACCACCACCATGGACAACAAGCCT; TGGGTAACCGGACCAGGCCAAGGGTCTACAATTCTCCAACGAATAGCTCCTCTACCCAAGATTCTATGG; AGGTTGGCCACAGTCACCACTCCATGACATCCCTGTCTTCCTCAACGACTTCTTCCTCGACATCTTCCT; CCTCTACTGGTAACCAAGGCAATCAGGCCTACCAGAATCGCCCAGTGGCTGCTAATACCTTGGACTTTG; GACAGAATGGAGCTATGGACGTTAATTTGACCGTCTACTCCAATCCCCGCCAAGAGACTGGCATAGCTG; GACATCCAACATACCAATTTTCTGCTAATACAGGTCCTGCACATTACATGACTGAAGGACATCTGACAA; TGAGGCAAGGGGCTGATAGAGAAGAGTCCCCCATGACAGGAGTTTGTGTGCAACAGAGTCCTGTAGCTA; GCTCGTGACTACATTGAAACTTGAGTTTGTTTCTTGTGTGTTTTTATAGAAGTGGTGTTTTTTTTCCAA; AAACAAAGTGCAAAGCTGCTTGAATCAGGAGGAGATTAACACACTGAACCGCTACAAGAGGGCAAAGCT; GATTTTTTTTTTAACTTGAAAAGATTGCAAAGGGACATTGAAGTGTTTAAAAGAGCCATGTCCAAACCC; ATCTTCATGGATAGCTCAGAGGTATCCTCTTTTTGCTCCCCCATTTTAACTTGCCACATCCCAGTCACA; GTGGGGTTTTTTTGTCTTTCTATTCAGCAAAAGTTAATATTCAGATGTTGGTCTTGGTCATTTGCCAAC; TAATTTTAAAGTAAAAGGCACTGCACATAATTTGCATAAAGGGCCCCATGAGGGTGTTTTTTTTTTTTC; TTTTTGTCCCCCCCATCCCCCTTTTTTTTTGTTTTGTTCTGTTTTGTTTTGGGTGGGAGGGTGGGAAAT; TTGGGTTTTTAAGTCCTCTAAACACACTTGGGCACGGAAATGCAGTACTGTAAGGAAGAGGGACCTCCA; GCTTCCACAAACACCATCTTCAGCTGTATGAAAGGGACGGTTGTGGTGAAGTTTGTCAGGCACAGTAAG; CATGCTGAGTGGCGGGGATCAGAACTCTCCTATCTGAACCTACTGAGGAGCAAAGCAGCAATTACATGG; GATCCTGTGGCTCTCCCGTTGCAGAGGCCACAGGAAGATAGGATGGAACGTGACTGGTCTCCTAACCAA; GGTGCACTGAGAAGCAATCAACGGGTCGGTCGTGGCCAGTCCTGGGGAGGTCTGAGTGGTGGTCTTTGG; GATAACCTTTGGCCTTATGGATTTGGACTCGAAATTAGAAGAGCCTACCATTTCAGATGCAATCACTTT; TGGACATGCTTTTGCAGACAGTCCTTAATGCTGAAAACACAGAGAATGGGTAATTCAAGAGGCCTTTCT; TTTAAAATAGACTTTTGTGACCCACTAATTGTAAGGTATTGCAAGGTCACTTTGCGTGTGTCATAAAGT; TGACTTCCTTATTGGTTGAAGGTCACAGAAGTAGTGGTTTGCTTTGATGGAAATAGCTACAGCTGTGTC; CCTTCCTGCTTTTTACTTTTTCTTTTGCTTTTTCTCGGCACGTGGTATCTCCACCATTTCTTCTGCACA; AAGATGTCTTCTGTTCATCCTGAACATTTTTAAAAAATGCAGAATTTTATGTGACTGCTTTTTTGCCTC; ACAATTATGCTGTGAATTTTACAAAAATTTATTTTCTTTTTTGATAATTTATTGTACCAAAGCTGTTT; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_101395.2
• Locus ID: 1859
• Summary: This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]