Gremlin 1 (GREM1) (NM_013372) Human 3' UTR Clone

SKU SC220862

3' UTR clone of gremlin 1 cysteine knot superfamily homolog (Xenopus laevis) (GREM1) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Gremlin 1
• Synonyms: C15DUPq; CKTSF1B1; CRAC1; CRCS4; DAND2; DRM; DUP15q; GREMLIN; HMPS; HMPS1; IHG-2; MPSH; PIG2
• ACCN: NM_013372
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC220862 3’UTR clone of NM_013372 ; The sequence shown below is from the reference sequence of NM_013372. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TGTCGTTGCATATCCATCGATTTGGATTAAGCCAAATCCAGGTGCACCCAGCATGTCCTAGGAATGCAG; CCCCAGGAAGTCCCAGACCTAAAACAACCAGATTCTTACTTGGCTTAAACCTAGAGGCCAGAAGAACCC; CCAGCTGCCTCCTGGCAGGAGCCTGCTTGTGCGTAGTTCGTGTGCATGAGTGTGGATGGGTGCCTGTGG; GTGTTTTTAGACACCAGAGAAAACACAGTCTCTGCTAGAGAGCACTCCCTATTTTGTAAACATATCTGC; TTTAATGGGGATGTACCAGAAACCCACCTCACCCCGGCTCACATCTAAAGGGGCGGGGCCGTGGTCTGG; TTCTGACTTTGTGTTTTTGTGCCCTCCTGGGGACCAGAATCTCCTTTCGGAATGAATGTTCATGGAAGA; GGCTCCTCTGAGGGCAAGAGACCTGTTTTAGTGCTGCATTCGACATGGAAAAGTCCTTTTAACCTGTGC; TTGCATCCTCCTTTCCTCCTCCTCCTCACAATCCATCTCTTCTTAAGTTGATAGTGACTATGTCAGTCT; AATCTCTTGTTTGCCAAGGTTCCTAAATTAATTCACTTAACCATGATGCAAATGTTTTTCATTTTGTGA; AGACCCTCCAGACTCTGGGAGAGGCTGGTGTGGGCAAGGACAAGCAGGATAGTGGAGTGAGAAAGGGAG; GGTGGAGGGTGAGGCCAAATCAGGTCCAGCAAAAGTCAGTAGGGACATTGCAGAAGCTTGAAAGGCCAA; TACCAGAACACAGGCTGATGCTTCTGAGAAAGTCTTTTCCTAGTATTTAACAGAACCCAAGTGAACAGA; GGAGAAATGAGATTGCCAGAAAGTGATTAACTTTGGCCGTTGCAATCTGCTCAAACCTAACACCAAACT; GAAAACATAAATACTGACCACTCCTATGTTCGGACCCAAGCAAGTTAGCTAAACCAAACCAACTCCTCT; GCTTTGTCCCTCAGGTGGAAAAGAGAGGTAGTTTAGAACTCTCTGCATAGGGGTGGGAATTAATCAAAA; ACCTCAGAGGCTGAAATTCCTAATACCTTTCCTTTATCGTGGTTATAGTCAGCTCATTTCCATTCCACT; ATTTCCCATAATGCTTCTGAGAGCCACTAACTTGATTGATAAAGATCCTGCCTCTGCTGAGTGTACCTG; ACAGTAGTCTAAGATGAGAGAGTTTAGGGACTACTCTGTTTTAGCAAGAGATATTTTGGGGGTCTTTTT; GTTTTAACTATTGTCAGGAGATTGGGCTAAAGAGAAGACGACGAGAGTAAGGAAATAAAGGGAATTGCC; TCTGGCTAGAGAGTAGTTAGGTGTTAATACCTGGTAGAGATGTAAGGGATATGACCTCCCTTTCTTTAT; GTGCTCACTGAGGATCTGAGGGGACCCTGTTAGGAGAGCATAGCATCATGATGTATTAGCTGTTCATCT; GCTACTGGTTGGATGGACATAACTATTGTAACTATTCAGTATTTACTGGTAGGCACTGTCCTCTGATTA; AACTTGGCCTACTGGCAATGGCTACTTAGGATTGATCTAAGGGCCAAAGTGCAGGGTGGGTGAACTTTA; TTGTACTTTGGATTTGGTTAACCTGTTTTCTTCAAGCCTGAGGTTTTATATACAAACTCCCTGAATACT; CTTTTTGCCTTGTATCTTCTCAGCCTCCTAGCCAAGTCCTATGTAATATGGAAAACAAACACTGCAGAC; TTGAGATTCAGTTGCCGATCAAGGCTCTGGCATTCAGAGAACCCTTGCAACTCGAGAAGCTGTTTTTAT; TTCGTTTTTGTTTTGATCCAGTGCTCTCCCATCTAACAACTAAACAGGAGCCATTTCAAGGCGGGAGAT; ATTTTAAACACCCAAAATGTTGGGTCTGATTTTCAAACTTTTAAACTCACTACTGATGATTCTCACGCT; AGGCGAATTTGTCCAAACACATAGTGTGTGTGTTTTGTATACACTGTATGACCCCACCCCAAATCTTT; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_013372.7
• Locus ID: 26585
• Summary: This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]