GLI2 (NM_005270) Human 3' UTR Clone

SKU SC217310

3' UTR clone of GLI family zinc finger 2 (GLI2) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: GLI2
• Synonyms: CJS; HPE9; PHS2; THP1; THP2
• ACCN: NM_005270
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC217310 3’UTR clone of NM_005270 ; The sequence shown below is from the reference sequence of NM_005270. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GAGAGCAAGTTCCTGAACATGATGACCTAGAGGCCCGAGCGCCTGGTGCTGAGTGCACCCGGAGGGGTC; ATCGCTGCCCAGAGCCTGGGGATTCCAGCTGTCTTGTCTTTTTCCAAAAAAGTGTTAAATAGGCTTGAG; GGGTTGTTGCGCAATGGCCGCTTCAGATGACAGATGTTGTAAGAGAAGGTTTATGGGCATCCTCTCTGG; TCTTTTGGATTATTCCTCAGAACAATGAAAAAAGTCTCCATAGGACAGGAAGGAATGCAAAACTCATTT; ACACAGTGCTTTCCAGCCTTTGGTGCTTACAGGACCGCGCTGTTCCGGCTTCTTCACGGCTGACATTCG; GCTAACGAGGGATTACTTTGGCCAAAACCTTTCAAAGGATATGCAGAAAGATGGTAGGGAGCATTTGGG; TTTGAATCTGAATGCTATACTGGATACTCTGCTCCGGAAAGATGAGCTTTTTATTCTACTACTTGGAAG; GAAAAGGAATTCCTGGTCCACCTGAATTCCTCTATGAAGCCTAACTCTTGAGGTCTCTAACATACCTTG; TCATAGAGGAAAAGCACAGATTATACCTGGATGATTCAGGAGCACATTCTGATTCCAGGTTTGGTAGAG; CTGGCTCTTCTACTCCGTAAAGCCGAGTCTGGGACTGGCAGCCCATCCAAGTGTATATGAATGAATAAA; GCATCCAAGTATATATGAATGAATAAAGTATGTAAGTATCACCAGAAAAAGGAAAGAAAAAATGTACTC; CTTGGGGCAAGCCCAGAAGCTGCCCTGGCCTCTCCAGACCGTGTTTACAGTGTTTGCATGTAGAATGTA; GCCCTTCCTGAAAAGAAGACTTGTTTCTAAATACCTCGGGGCTGCTGGAGCCGCTGTGGGTTAGGGATG; GACTGAGGCCTCGAGGAGTGAGGGTGCACCCGGGGCCCAGCCTCAGGCTGCCCTAGGGATCTCTCAGTA; GGAAGAGGAAGTTGCGTGTTTACCCAATCCTGTTTCTCCAATGCAACGTCCACCCACTTTACCACCAAA; AACTCCAGGGCCTGACGGCAGCCCGGTCCCCCAGCACTCACCAGCAGCCCAGTGTTCTCCACCAAGCCA; CAGTGTGCATGCCTGGTATCCTCCGGATTCCCTTCCTTCTGCCCGCTGAGTCACTGGGCAGAGAATGAT; GACATGTGTAGGTGGTGTGGTTGGGGGTGGAAAGGGGAAGGGGTTGATCCTCAGGACTCTGAGGGAGCA; TCGTTGAATTTTCCTGTTCAGTGTGACCAAGACCCACCTGGAAATGGAATTTGGAACTGGCTTCAGGAG; ACATCATTCCTGAACACACTGTAGGGTGAATTGGTGCATCTTCCCCACCATACACACACACACACACAC; ACACACACACACACACACACACACACCCCAAACCTTTTCATGGGGAATGTGTGGCAACCTTGCCAAACA; GCACCACTCAGAGTGTGACTCTGACTGTGACCTTGGCCTTAATGAGGAACTTCTTAGGAGAGTTTGAGG; ACAAGGCCAACATCGTCATCTGGGCTCGCTGCGTCCCAGCACATCAAACTCTGTCCAGAGACAAGGCCA; ACTGCAAATGAAAGCCAGGGAACATTGCTAAGGGTCTGTGGCTCTGTGGTGGTGTTCATCGCCTTCCTG; AGATAGGATTTCCCTTGCCAGTCCCAACCTGTATATATTCTGTACAGAAGACATCCCTGAATATACTGT; AGGTGAGTCGTCCAGCCAAATTTATATCTCCAAAACATTTTTAGCTTTTTCTACATGCTATGAATTGAG; ATGACATGCTCAACTTGTAAATAAGTCTTTTTGTACATTAAAAAAGTAATTTTTTCATAATTTATCTTG; TCTATCTGCTTCCCCCTTGACAGTAGTTAATGAGAACCTGGGCAGTAAATTTGGTGCATTCGAGCAGAA; ATTAGGCTGTATTTTTTCTTAACAGTGTCAAAATTGACTATCCCGCCTTTGCCAAGAAATGTTTAATG; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005270.5
• Locus ID: 2736
• Summary: This gene encodes a protein which belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors which bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]