Klotho (KL) (NM_004795) Human 3' UTR Clone

CAT#: SC217236

3' UTR clone of klotho (KL) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: Klotho
• Synonyms: HFTC3
• ACCN: NM_004795
• Insert Size: 1987 bp
• Sequence Data:
  Insert Sequence

  >SC217236 3’UTR clone of NM_004795
  The sequence shown below is from the reference sequence of NM_004795. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TCGAAGAAAGGCAGAAGAAGTTACAAATAGTTCTGAACATTTTTCTATTCATTCATTTTGAAATAATTA
  TGCAGACACATCAGCTGTTAACCATTTGCACCTCTAAGTGTTGTGAAACTGTAAATTTCATACATTTGA
  CTTCTAGAAAACATTTTTGTGGCTTATGACAGAGGTTTTGAAATGGGCATAGGTGATCGTAAAATATTG
  AATAATGCGAATAGTGCCTGAATTTGTTCTCTTTTTGGGTGATTAAAAAACTGACAGGCACTATAATTT
  CTGTAACACACTAACAAAAGCATGAAAAATAGGAACCACACCAATGCAACATTTGTGCAGAAATTTGAA
  TGACAAGATTAGGAATATTTTCTTCTGCACCCACTTCTAAATTTAATGTTTTTCTGGAAGTAGTAATTG
  CAAGAGTTCGAATAGAAAGTTATGTACCAAGTAACCATTTCTCAGCTGCCATAATAATGCCTAGTGGCT
  TCCCCTCTGTCAAATCTAGTTTCCTATGGAAAAGAAGATGGCAGATACAGGAGAGACGACAGAGGGTCC
  TAGGCTGGAATGTTCCTTTCGAAAGCAATGCTTCTATCAAATACTAGTATTAATTTATGTATCTGGTTA
  ATGACATACTTGGAGAGCAAATTATGGAAATGTGTATTTTATATGATTTTTGAGGTCCTGTCTAAACCC
  TGTGTCCCTGAGGGATCTGTCTCACTGGCATCTTGTTGAGGGCCTTGCACATAGGAAACTTTTGATAAG
  TATCTGCGGAAAAACAAACATGAATCCTGTGATATTGGGCTCTTCAGGAAGCATAAAGCAATTGTGAAA
  TACAGTATACCGCAGTGGCTCTAGGTGGAGGAAAGGAGGAAAAAGTGCTTATTATGTGCAACATTATGA
  TTAATCTGATTATACACCATTTTTGAGCAGATCTTGGAATGAATGACATGACCTTTCCCTAGAGAATAA
  GGATGAAATAATCACTCATTCTATGAACAGTGACACTACTTTCTATTCTTTAGCTGTACTGTAATTTCT
  TTGAGTTGATAGTTTTACAAATTCTTAATAGGTTCAAAAGCAATCTGGTCTGAATAACACTGGATTTGT
  TTCTGTGATCTCTGAGGTCTATTTTATGTTTTTGCTGCTACTTCTGTGGAAGTAGCTTTGAACTAGTTT
  TACTTTGAACTTTCACGCTGAAACATGCTAGTGATATCTAGAAAGGGCTAATTAGGTCTCATCCTTTAA
  TGCCCCTTAAATAAGTCTTGCTGATTTTCAGACAGGGAAGTCTCTCTATTACACTGGAGCTGTTTTATA
  GATAAGTCAATATTGTATCAGGCAAGATAAACCAATGTCATAACAGGCATTGCCAACCTCACTGACACA
  GGGTCATAGTGTATAATAATATACTGTACTATATAATATATCATCTTTAGAGGTATGATTTTTTCATGA
  AAGATAAGCTTTTGGTAATATTCATTTTAAAGTGGACTTATTAAAATTGGATGCTAGAGAATCAAGTTT
  ATTTTATGTATATATTTTTCTGATTATAAGAGTAATATATGTTCATTGTAAAAATTTTTAAAACACAGA
  AACTATATGCAAAGAAAAAATAAAAATTATCTATAATCTCAGAACCCAGAAATAGCCACTATTAACATT
  TCCTACGTATTTTATTTTACATAGATCATATTGTATATAGTTAGTATCTTTATTAATTTTTATTATGAA
  ACTTTCCTTTGTCATTATTAGTCTTCAAAAGCATGATTTTTAATAGTTGTTGAGTATTCCACCACAGGA
  ATGTATCACAACTTAACCGTTCCCGTTTGTTAGACTAGTTTCTTATTAATGTTGATGAATGTTGTTTAA
  AAATAATTTTGTTGCTACATTTACTTTAATTTCCTTGACTGTAAAGAGAAGTAATTTTGCTCCTTGATA
  AAGTATTATATTAATAATAAATCTGCCTGCAACTTTTTGCCTTCTTTCATAATCA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004795.4
• Summary: This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]
• Locus ID: 9365
• MW: 77.6