Klotho (KL) (NM_004795) Human 3' UTR Clone

SKU SC217236

3' UTR clone of klotho (KL) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: Klotho
• Synonyms: HFTC3
• ACCN: NM_004795
• Insert Size: 1987 bp
• Sequence Data:
  Insert Sequence

  >SC217236 3’UTR clone of NM_004795 ; The sequence shown below is from the reference sequence of NM_004795. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TCGAAGAAAGGCAGAAGAAGTTACAAATAGTTCTGAACATTTTTCTATTCATTCATTTTGAAATAATTA; TGCAGACACATCAGCTGTTAACCATTTGCACCTCTAAGTGTTGTGAAACTGTAAATTTCATACATTTGA; CTTCTAGAAAACATTTTTGTGGCTTATGACAGAGGTTTTGAAATGGGCATAGGTGATCGTAAAATATTG; AATAATGCGAATAGTGCCTGAATTTGTTCTCTTTTTGGGTGATTAAAAAACTGACAGGCACTATAATTT; CTGTAACACACTAACAAAAGCATGAAAAATAGGAACCACACCAATGCAACATTTGTGCAGAAATTTGAA; TGACAAGATTAGGAATATTTTCTTCTGCACCCACTTCTAAATTTAATGTTTTTCTGGAAGTAGTAATTG; CAAGAGTTCGAATAGAAAGTTATGTACCAAGTAACCATTTCTCAGCTGCCATAATAATGCCTAGTGGCT; TCCCCTCTGTCAAATCTAGTTTCCTATGGAAAAGAAGATGGCAGATACAGGAGAGACGACAGAGGGTCC; TAGGCTGGAATGTTCCTTTCGAAAGCAATGCTTCTATCAAATACTAGTATTAATTTATGTATCTGGTTA; ATGACATACTTGGAGAGCAAATTATGGAAATGTGTATTTTATATGATTTTTGAGGTCCTGTCTAAACCC; TGTGTCCCTGAGGGATCTGTCTCACTGGCATCTTGTTGAGGGCCTTGCACATAGGAAACTTTTGATAAG; TATCTGCGGAAAAACAAACATGAATCCTGTGATATTGGGCTCTTCAGGAAGCATAAAGCAATTGTGAAA; TACAGTATACCGCAGTGGCTCTAGGTGGAGGAAAGGAGGAAAAAGTGCTTATTATGTGCAACATTATGA; TTAATCTGATTATACACCATTTTTGAGCAGATCTTGGAATGAATGACATGACCTTTCCCTAGAGAATAA; GGATGAAATAATCACTCATTCTATGAACAGTGACACTACTTTCTATTCTTTAGCTGTACTGTAATTTCT; TTGAGTTGATAGTTTTACAAATTCTTAATAGGTTCAAAAGCAATCTGGTCTGAATAACACTGGATTTGT; TTCTGTGATCTCTGAGGTCTATTTTATGTTTTTGCTGCTACTTCTGTGGAAGTAGCTTTGAACTAGTTT; TACTTTGAACTTTCACGCTGAAACATGCTAGTGATATCTAGAAAGGGCTAATTAGGTCTCATCCTTTAA; TGCCCCTTAAATAAGTCTTGCTGATTTTCAGACAGGGAAGTCTCTCTATTACACTGGAGCTGTTTTATA; GATAAGTCAATATTGTATCAGGCAAGATAAACCAATGTCATAACAGGCATTGCCAACCTCACTGACACA; GGGTCATAGTGTATAATAATATACTGTACTATATAATATATCATCTTTAGAGGTATGATTTTTTCATGA; AAGATAAGCTTTTGGTAATATTCATTTTAAAGTGGACTTATTAAAATTGGATGCTAGAGAATCAAGTTT; ATTTTATGTATATATTTTTCTGATTATAAGAGTAATATATGTTCATTGTAAAAATTTTTAAAACACAGA; AACTATATGCAAAGAAAAAATAAAAATTATCTATAATCTCAGAACCCAGAAATAGCCACTATTAACATT; TCCTACGTATTTTATTTTACATAGATCATATTGTATATAGTTAGTATCTTTATTAATTTTTATTATGAA; ACTTTCCTTTGTCATTATTAGTCTTCAAAAGCATGATTTTTAATAGTTGTTGAGTATTCCACCACAGGA; ATGTATCACAACTTAACCGTTCCCGTTTGTTAGACTAGTTTCTTATTAATGTTGATGAATGTTGTTTAA; AAATAATTTTGTTGCTACATTTACTTTAATTTCCTTGACTGTAAAGAGAAGTAATTTTGCTCCTTGATA; AAGTATTATATTAATAATAAATCTGCCTGCAACTTTTTGCCTTCTTTCATAATCA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004795.4
• Locus ID: 9365
• Summary: This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss. [provided by RefSeq, Jul 2008]