POLD3 (NM_006591) Human 3' UTR Clone

SKU SC217193

3' UTR clone of polymerase (DNA-directed) delta 3 accessory subunit (POLD3) for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: POLD3
• Synonyms: P66; P68; PPP1R128
• ACCN: NM_006591
• Insert Size: 2000 bp
• Sequence Data:
  Insert Sequence

  >SC217193 3’UTR clone of NM_006591 ; The sequence shown below is from the reference sequence of NM_006591. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; TCCATTACTGGCTTCTTCCAGAGGAAATAAACTGCCATCTCTGGTAGATCAGAGACTTGGAGTGGTCAA; GGGAGAAGACCAAGAAATGTACTCCTCACTTACTATGTAAGTTCATCTAGATCTCCACCTCACCTGTAT; CAAAAGACTGTTCTTTCATCCTGTGAGGTTTATACTATTTCTGGTTTTTAACCAAAAGGAAATCATCTG; GAAGCAGGAGGCAAAAAGCTGTTACCTTCTAATGACATTTAAAAAGCACAGTCTTTGACCTGTCCAGGA; GAAGGATTTACTCCAAAATTATACTGGAACAGTTTTCAGAATTCTCACTGAAGCCATTTAGTGGCTAAC; CCACTGTGCTCCACTCACCCTATGCCCTGGTCCGCATATGGCACAGGAATTATTCCTTCTTGTTCCTCT; GTATTCTAAGAATTCATGTGGGTGTTTCTTATCCTTACATTCTGCTGGATACGTTTACCCCTCTTGTCT; TCCTGCAGTACCACACTTCTGTCCCCTAACCTCCTGAGGCTGTTCTCTGTAAGTCTTTTAAGTTTTGGT; TGGACTAAAGGCTGAAGTGAAAATCTCCCTGTAATCCTCTTCCTCCATTATGCAGTACACGGACACCTG; GCTACAGACCAGGACGTGGCTTGTGTTCTGTTCCTTTCACAAAAGCTCTCTGGGACCTTCACTTGCAAT; TAGTGGTTAGGGAAAAGCCTCAGGCAGAACACAAATAGAAATTTAATGATGTGTTCAACTCCACCAGAA; ATTACCTCGAGTCAGCATTGACGATATTGGAGGAGCTGCCGTGCTGCTGATACGGGGTGTGCTTTATGC; TGCTCTTTGCGGTTTGTTGATCCCCTCCTCCCCCACTCTCAATACCTAGAGAGTGAAACCCGTACAATG; AGATAAAGACTAAAAAGAGAAATCCCTTCCTATATACAGTGTGCTACATTTACAAAAAATTTCTCCTTA; AGAAAACAGAATATTCAAAAACAGCACTTTTCCAGGAAGTTAGTGAGGAAGATAAGGCACACATTTATT; TATAAATGGATGTTGCTCCTTTGTATTTTTAGCTTCCCTATTTGCTTAAAAGTACAGGCTCCCTAAGAG; AGGATGGAGAGGGAGAGACTGAATTGTTAGTAGGTCTAAACATCAAAGAAAACATTTTTATTAGTTACT; TATGGAAAATCATCTATTACAATGATAACCTTCAAGTGACTTCCATTATGGCTGGACAGGCGGTGAGCT; CAGTGGATTGCAGTGGTGTGCTGGTGATTTTGCACAGTGAGCTCTGCGGAAGGGGTCAGGCTCAACTGC; TGATGTGTCTCACACGTAGCAGACAAGGGGTGTCTGACTGGCTTCTTTTGCCTCAAGATGGTGTATGTC; GTAAGTGATGCAATCAGCTGTCTGCTTTTTAAGGTTGGGATTGTGCTGACTTTGGGATTAACATGAGCT; TCTTTAGCAACCAAGCATGAACTTGATTAAGACCAGAAGTTTGGGAGATGAGTCCTGGCATTATGTCTA; GGACTAAAGCAGTGGCTTTGTATAGCAAGCTGAGTAAAGGTTGACATATTCCAAAACCCTTCTTTTTAA; AAGGAAAAAGGATGGAGAGAAGGATGGAAAGCCTGGACTTAAACCTTTAGAAAAAACTTCTGGAGAGAA; ATCCCTTTTAAACAGTTACTTTTGTCATTGCCTCTGGTCATTTGTCTAAATAGGAATGGAAAATTAAAA; GAAAAGCAACAATCCAATCTTTTTTCTAAAAATTATGCTGGGGTCTCGACTAAAACTGAATTTGAATTG; GAAAATTCTGGTGTTGGTTGGAGTTCCATCTTGCAAGGGATAATACAAATCCTATGATCTCTATGCCCA; ATATGCTGCCTCAACTCTGAGCTGTCTGCAAGGCTTAGTAAGTATTGAGTGGTGTTTTTTTTTTCTTTT; TATACAATACCATGTTAACCACATGAGTTAAATAAATTTGAGAAGTTGTTTTAAAACAGTGCTTCAAA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006591.3
• Locus ID: 10714
• Summary: This gene encodes the 66-kDa subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein plays a role in regulating the activity of DNA polymerase delta through interactions with other subunits and the processivity cofactor proliferating cell nuclear antigen (PCNA). Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Mar 2012]