UBE3B (NM_130466) Human 3' UTR Clone

SKU SC217138

3' UTR clone of ubiquitin protein ligase E3B (UBE3B) transcript variant 1 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: UBE3B
• Synonyms: BPIDS; KOS
• ACCN: NM_130466
• Insert Size: 1950 bp
• Sequence Data:
  Insert Sequence

  >SC217138 3’UTR clone of NM_130466 ; The sequence shown below is from the reference sequence of NM_130466. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; AGCATGAACACGGGCTTTGAACTCTCCTAGCTCCTGTCCCAGCCCTGCCTCCAGGGCTCCTGGGCTGCC; AGGGACCTTCAGCTCCCAGAGGCAGTGTGGTCCTGGGAATGTGACCAACATGCCAGGTGACATTGGCCC; CTAGACCCTCTCTATAGCCATGAGACTCCTTGTGGCCTCAAGAAATTTAGACGCCCACGACAGCACTAC; ACAGCATCTCCAGGTGATGCCCAAGGCACAGGGCTGCAGAAAATAAACCTCCAGATTCCACCAACACGG; GTCCATTCTTCCTGGTGATGGCAGAGGGGCTTCTTTTAGCTAGTTTGATCTTTTGGGAGTCTGTCTTTC; CTTAGCCGTCTGAGTGAGCTGTGTATGAACAAGTCCCAGGAGTTCCAAGAGTCTAGAGTGGTTTTTGCA; GCATGGGTTGAGTGTACAAAGCCTACTGTGCGTGAGATCCTCTCCTTCCGTTTCTGAAATCTCTTACTC; AGGTAAGGCCTCGCCAAGCCTCTATGCACCCCACAAAGTTTCTGCCTCCATGCCGTCCACAGCGCCTCT; TCCCAGACAGCCAGGCCCATCTGCTGCCCAGGGAAGCGCAGGCGCCTGCTAGGGACGCTATGGACACCG; TGAGTCCAAGGCGCTGCTCCTGCCTTGAAGCCACGCGCTCCACGCCGCGGCCCTCCCATTTTCTGCGTC; CTCAGCGGGCTGAGCTGCCAGAGAGTCTTCCCGGACCTATTCCCGTCCTATGCATTCACATTGGCATCC; TGGTTTGGGGGAAGAAAAACAACGGCCCTTAGCAGCAGCCCCGTTTCCAGAATGTGCTGCCTGTTCCCC; AAAGCCTGCTTGTCCCGCGGAGGACGGCTGCCTTTGACCCTGCTTATTTGTCTCACTGGTTATCTAATG; AGGAACAAACACTAACCTAAGGTACACATCCCATCTGGGCGGTGGCTTCACTCCTGAGTCTGCAAAATC; CCAGGAAACTTGGGTCTTGCTGCCCATCCTTCCTCAGCACAGGGAACCCGGAAGCCCGTTGCACTGACA; GAGGCTCACACCCTCTGGGTTTTTTGTTTTTTTTTTAAACTTCATTTCTCTTCCAACCCATTGTGTTCC; TCTGCCCTCATTCTTTACCTTTGTTGTAAGAACTTTAGCTCCAGGGAACTGAGGCAGAGGCCCTGTTTG; TGGTCTGTGTTGACAGCCACCCACCCTCTCCCACCTCATGCTCCTGCACCTGCACCACCAAGGTTGATG; CCGGATTCGAAGCCAGGAAGGCCCAGTCCCTCCTCTGCTCTCCTCAAAGACGCAAAACATTTTCCAACA; AGAGCTGTCACAGTGGCAGCAAAGCAGGGGCCACCCGTCTCCACAGTCCTTCAGAGTTTCAGCTGCATC; CTGGTGTCCCACCCAGGTCGAGCCCCCAGGCTTCTGGAAAAGAGTGTGTGCTCTACTTTGATGGAAACA; TGGCAAGGAATTTAAAGACAGGAATGTATTAATATTATTGAAGGTGTGTTCGTAACCTCTGATTCTGTG; GACTTGCCACTTTCTCCAAACGCTCGGTTCCTTTGAAGATTTCTTCTGAACGTGTGTGCGCACGCTGGG; CGGGTTCGTGCATACATGCGGGGACCCCAGACTGTCAGCACAGGGAAGATGGATCCCATCCTAATTTTT; ATCACCTGAAGGTTGGAACCAGTGAGGGACTGGGAGAGAGTGATTTATAAGCACCAATATCAACTTCAT; GTGGATTTTTGACAAGGAGGGGTAGTTTGTAATTTCATTTAAATTCTTTTCAGCAGCTGGAAATATTGC; ACTATCTGAAACACTGAATCTCCTTTTGTAACTGGTGTTCACTGACACCTTGATGGCTCTTGATGGCTC; TAAAAAGTTGTAGGATTTTTTGTTTTTGTAGCTAACTTATGGATTGAGATGTGATCAAAGGCTTTATTA; AATTTGTACTTCAGCATA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_130466.4
• Locus ID: 89910
• Summary: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2012]