PDCD4 (NM_145341) Human 3' UTR Clone

CAT#: SC217110

3' UTR clone of programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PDCD4
• Synonyms: H731
• ACCN: NM_145341
• Insert Size: 1948 bp
• Sequence Data:
  Insert Sequence

  >SC217110 3’UTR clone of NM_145341
  The sequence shown below is from the reference sequence of NM_145341. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  GGAGGTCGTCTTAAACCAGAGAGCTACTGAATATAAGAACTCTTGCAGTCTTAGATGTTATAAAAATAT
  ATATCTGAATTGTAAGAGTTGTTAGCACAAGTTTTTTTTTTTTTTTTTTTTAAGCACTTGTTTTGGGTA
  CAAGGCATTTCTGACATTTTATAAACCTACATTTAAGGGGAATTTTTAAAGGAAATGTTTTTTCTTTTT
  TTTTTGTTTTTCGAGGGGGCAAGGAGGGACAGAAAAGTAACCTCTTCTTAAGTGGAATATTCTAATAAG
  CTACCTTTTGTAAGTGCCATGTTTATTATCTAATCATTCCAAGTTTTGCATTGATGTCTGACTGCCACT
  CCTTTCTTTCAAGGACAGTGTTTTTTGTAGTAAAATCACTGGTTTATACAAAGCTTTATTTAGGGGGTA
  AAGTTAAGCTGCTAAAACCCCATGTTGGCTGCTGCTGTTGAGATACTGTGCTTTGGGAGTAAAAAAAGA
  AAGTTATTTCTTTGTCTTAAAGAATTTTTAAAAAATTAGTCATGAGACTTATTCATCTTTCCAGGGAAC
  ATACTGATTGGTCTTAAAAGACTAGACAGTTAAGTAAAAGGTGGCTGGAACATCTATTTTTCTACAAAA
  CTGGAAAAATGAACCTGGTTCTAGAAGAATGTACACCAAAATAAAACATGTGAAGCAGTATTGATTCTT
  TATTGGGAGTACATTTTTTTAGGTCTCTTAAACTTTAATTTCACACAGTAAATTTTGAATCTCATAAGG
  AAGCATATTTGAACCTAGTCAATTTAATCTTAGTGTTCCCTTGAAAACTTTTTTTCCCTACAAAATTTT
  AAGTGAAAAATACAATAGTAAATTAAGATTACACTGGGGAAAAAAATGCAGGTATCACTTTACTCCATT
  GTTATCTGACCTAGAGCTTAATTAAGTTTTAGAAATATGTAATACCTTCCATCATTCCATCATCCTTAA
  ATTCTGTTACCAAATAATGGCTAATGTTACAAAAAGTTATACTCCAGAGACCCAAAGCTTGACATTTAC
  CTAATGTATGAGAAAATATTACCAATTAACAATAAAGAATGATCATATTTTTAACCTCTTTTACATAGC
  CTAATAACTCAGCAAGGCCTCAACGTCTGTGCTAATTTAAACTGCCAAATATTGACTGCAGCAAACAAG
  AATTATATTCAGAATTTATGAGGGTACTGTTAGGAGTATACTGCTTACAGGTTTAGATATAGTCTGTTA
  GAATTAAAACCAAGTTTAGTGTTCATATTTACCTCATGGGCTTTATCAAGCCCATATTACCTCAGCTTA
  TATATAGTTACCATTTTTAGGTTTTTAATTGTTTGACACTTGGATGATAAATGCAGTCATTTTATTCTC
  AAGTGCTTAAAATTAATGTAATTAAAAGCTTAGCTGACTACAGAATAGGTGAGGGTTTCTTAAAAATGA
  GATTTAAGGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGAT
  CACTTGAGGTTGGGAGTTCATGACCAGCTTGACCAACATGAAGAAACCCTGTCTCTATTAAAAATACAA
  AAGTAGCCAGGCATGGTGGCGCATGCGTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATTGCT
  TGAACCTGGGAGGCAGAGGTTGCAGTGAGTCGAGATGGTGCCATTGCTCTCGTTTGGGCAACAAGAGTG
  AAACTCTTGTCTCAAAAAAAAAAAAAAATGAGGTTTAAGACAGTTTTGTCATTACTGGTGGGATCTGGT
  CACACAAGATAGCATTAAACGTGACATGGCACATAAAATTGGTTAAAAAATTTTGTTTTTTAATTACGT
  AATGTAAAAGCCCAACAAACACTTTATGCAAGATTGGAATGTATCTTCAAATTCAGATTTAATAAACAT
  GTAAAGATCCTCTGTA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_145341.4
• Summary: This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]
• Locus ID: 27250
• MW: 75.7