PDCD4 (NM_145341) Human 3' UTR Clone

SKU SC217110

3' UTR clone of programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4) transcript variant 2 for miRNA target validation

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Target Symbol: PDCD4
• Synonyms: H731
• ACCN: NM_145341
• Insert Size: 1948 bp
• Sequence Data:
  Insert Sequence

  >SC217110 3’UTR clone of NM_145341 ; The sequence shown below is from the reference sequence of NM_145341. The complete sequence of this clone may contain minor differences, such as SNPs.; Blue=Stop Codon Red=Cloning site; ; GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG; TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC; GGAGGTCGTCTTAAACCAGAGAGCTACTGAATATAAGAACTCTTGCAGTCTTAGATGTTATAAAAATAT; ATATCTGAATTGTAAGAGTTGTTAGCACAAGTTTTTTTTTTTTTTTTTTTTAAGCACTTGTTTTGGGTA; CAAGGCATTTCTGACATTTTATAAACCTACATTTAAGGGGAATTTTTAAAGGAAATGTTTTTTCTTTTT; TTTTTGTTTTTCGAGGGGGCAAGGAGGGACAGAAAAGTAACCTCTTCTTAAGTGGAATATTCTAATAAG; CTACCTTTTGTAAGTGCCATGTTTATTATCTAATCATTCCAAGTTTTGCATTGATGTCTGACTGCCACT; CCTTTCTTTCAAGGACAGTGTTTTTTGTAGTAAAATCACTGGTTTATACAAAGCTTTATTTAGGGGGTA; AAGTTAAGCTGCTAAAACCCCATGTTGGCTGCTGCTGTTGAGATACTGTGCTTTGGGAGTAAAAAAAGA; AAGTTATTTCTTTGTCTTAAAGAATTTTTAAAAAATTAGTCATGAGACTTATTCATCTTTCCAGGGAAC; ATACTGATTGGTCTTAAAAGACTAGACAGTTAAGTAAAAGGTGGCTGGAACATCTATTTTTCTACAAAA; CTGGAAAAATGAACCTGGTTCTAGAAGAATGTACACCAAAATAAAACATGTGAAGCAGTATTGATTCTT; TATTGGGAGTACATTTTTTTAGGTCTCTTAAACTTTAATTTCACACAGTAAATTTTGAATCTCATAAGG; AAGCATATTTGAACCTAGTCAATTTAATCTTAGTGTTCCCTTGAAAACTTTTTTTCCCTACAAAATTTT; AAGTGAAAAATACAATAGTAAATTAAGATTACACTGGGGAAAAAAATGCAGGTATCACTTTACTCCATT; GTTATCTGACCTAGAGCTTAATTAAGTTTTAGAAATATGTAATACCTTCCATCATTCCATCATCCTTAA; ATTCTGTTACCAAATAATGGCTAATGTTACAAAAAGTTATACTCCAGAGACCCAAAGCTTGACATTTAC; CTAATGTATGAGAAAATATTACCAATTAACAATAAAGAATGATCATATTTTTAACCTCTTTTACATAGC; CTAATAACTCAGCAAGGCCTCAACGTCTGTGCTAATTTAAACTGCCAAATATTGACTGCAGCAAACAAG; AATTATATTCAGAATTTATGAGGGTACTGTTAGGAGTATACTGCTTACAGGTTTAGATATAGTCTGTTA; GAATTAAAACCAAGTTTAGTGTTCATATTTACCTCATGGGCTTTATCAAGCCCATATTACCTCAGCTTA; TATATAGTTACCATTTTTAGGTTTTTAATTGTTTGACACTTGGATGATAAATGCAGTCATTTTATTCTC; AAGTGCTTAAAATTAATGTAATTAAAAGCTTAGCTGACTACAGAATAGGTGAGGGTTTCTTAAAAATGA; GATTTAAGGGCTGGGCACGGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGCGGAT; CACTTGAGGTTGGGAGTTCATGACCAGCTTGACCAACATGAAGAAACCCTGTCTCTATTAAAAATACAA; AAGTAGCCAGGCATGGTGGCGCATGCGTGTAATCCCAGCTACTTGGGAGGCTGAGGCAGGAGAATTGCT; TGAACCTGGGAGGCAGAGGTTGCAGTGAGTCGAGATGGTGCCATTGCTCTCGTTTGGGCAACAAGAGTG; AAACTCTTGTCTCAAAAAAAAAAAAAAATGAGGTTTAAGACAGTTTTGTCATTACTGGTGGGATCTGGT; CACACAAGATAGCATTAAACGTGACATGGCACATAAAATTGGTTAAAAAATTTTGTTTTTTAATTACGT; AATGTAAAAGCCCAACAAACACTTTATGCAAGATTGGAATGTATCTTCAAATTCAGATTTAATAAACAT; GTAAAGATCCTCTGTA; ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA; CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG;
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_145341.4
• Locus ID: 27250
• Summary: This gene is a tumor suppressor and encodes a protein that binds to the eukaryotic translation initiation factor 4A1 and inhibits its function by preventing RNA binding. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2010]