sphingosine 1 phosphate phosphatase 1 (SGPP1) (NM_030791) Human 3' UTR Clone

CAT#: SC216952

3' UTR clone of sphingosine-1-phosphate phosphatase 1 (SGPP1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: sphingosine 1 phosphate phosphatase 1
• Synonyms: SPP-1; SPPase1
• ACCN: NM_030791
• Insert Size: 1919 bp
• Sequence Data:
  Insert Sequence

  >SC216952 3’UTR clone of NM_030791
  The sequence shown below is from the reference sequence of NM_030791. The complete sequence of this clone may contain minor differences, such as SNPs.
  Blue=Stop Codon Red=Cloning site
  
  GGCAAGTTGGACGCCCGCAAGATCCGCGAGATTCTCATTAAGGCCAAGAAGGGCGGAAAGATCGCCGTG
  TAACAATTGGCAGAGCTCAGAATTCAAGCGATCGCC
  TACATATTTTTCTTTATTGGTATCTCTTGATGGAGAAGTATTGTTTATGATAAGAAAGGAGGGTATCAG
  TTACTGATACCCAAAAATATATTCCAGGTAAAGCCAGGTCAGAATTAGGCTTTTGCAGGAATTTAACTT
  AAATAATTATTTAAGTAAATTCATAAGAGTCAGTGCATTTTATCATTGCACATCCAGATACTGTTTTAG
  GTGAGCTGAGCTATTTCGTTACTGAGAAAATGATAAGTATCCATTTAGAATGTTCATGTAATGTTTGGA
  GAGTTTTGTATTATGGATTCAACTTATATATAATATATATAAGGTACATAATATGCAATTATATATCTG
  ATATATATTATATATAAAATAATATACCTAAGTGTTTTTTTAAACTATGGGAGTGTATTATAAAATCAA
  TAATAATATGCAAACAGTTGTGCCTGTGTATTTGAACTACATACAGGTATGTTGTTATTAACAGATATA
  TTTAACATTTATTTACTATGGGAGCCATTTCCTAATTGAATTGCGTAATTACTAGACCAGAATGCATAT
  GCTACTATACATTGATTTACTGCCTGTTTTTACACTGCCATTACCTTTCATGTTATCCATGATGTTGAA
  CATGGGAGGCATTTTTAATGGACCAAATTTTTAGAAAAATTAGTTTTTGGATTTTTTTTTAAGTTCTGT
  ATACTGTTTTGAATGAACTTTATTTGCAGCTATTCTGGATGTCAGTTTAATAATTCAGGTACTGAATTT
  TATTGCTTGCTAATTGTGCCTTTCCGTTGCTAAATTAAGAAATGCTATGTGTACTGTGATGTAAAAATA
  AGACATTAACTTTATCTTAAGTTACATATAATCAAGCAAATATTTTAAAAAATGTATGTCAGGGTGACA
  GGCATAAAAGTAAATTACCCTTAACTCTTTAGCCTTTATTTAGAAAGAAATGTGGAATGGGCTAAACTT
  TCTCACCAATTTATTAGAAAGCTAAATGGATAAATACTTCCCTTTTTGTAGATATTTTTCTGTGCATTG
  TAAGTTATAGGAGCAGGACATATTTTTTGTACATTGTCTTGATTGTTTATACTGCAGGTAGGTATTTCC
  AGTGAATGGAAACGTTGAAATTTATAGATGGGACAATGCACATACTTCATATGTAAACAAAGCATTTTT
  CTAAATAATTTGGAAAGGATTGGATTTAGCACCGTTAACTTTTAATACCCATTTATTGGCTATTTAAGG
  TAAATTATAAAACCTAAAATTAGTATCAGTATTTTAGATCCTATTATTTTTTCAACTTTCTATTATTTT
  CACAGTGGAAAATTTGCGTATAGTATTAAACTATTCTTTTTTCTGTCTACCTTTATATTTTGAAGTGTG
  GTTGTACTTACGTTAATAGTAAGTGTATTTGTAGAAAGTTTTTCATAAAGAAGAGATTAACTTTCCATA
  TAAATAAATACCAGGTATGAAATTACTGATTTTAGTGCAATATCTGTATGATTATCCAGAACTATCACT
  TCAGATGTTTCTATTTTGGGCACCATAACTATTTGAGCTCATATTTTTATAATTCTGATTGAGAATTTA
  GGGGCATGGTAATTTTTACCAAAACTGAAGCTGGTAATTTTAAGTAAAGCAACTAAATTTACCCTTTAC
  TTATTAGTTAAAAGGAAACCAAGGCATGTAGGAAAATATTGGTACGTTGGAAAATGTGAAAAGAGCTTT
  ACTTTCAAAATGTATGTTTAATGAATTGAATTTTAGGGACCGTTTCCCATAATTTGGGCCTATGCTATC
  CTGTTGATTTTTATGTCTAGCTTAAAAGGTATTTGAAAATGTTCCATTTGTGTTTTATTATTTTCTGTC
  CTATGACAGTTCTATGCTAAATGTATTTTAAAATAAAAGCCAAATTGAAGATTAAA
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCGACCAAGCGACGCCCAACCTGCCATCA
  CGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGG
  
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_030791.4
• Summary: Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]
• Locus ID: 81537
• MW: 74.7